Abstract: A method for making a coating composition by mixing one or more latex polymers and an aliphatic epoxy resin system having an epoxy equivalent weight less than 1000 and being distinct from the one or more latex polymers to provide an aqueous first component, and providing a second component having reactive groups that can react with the one or more epoxy resins. A mixture of the first and second components provides a film-forming curable coating composition that may be used on substrates including cement, cement fiberboard, wood, metal, plastic, ceramic, glass and composites.

Abstract: A hydraulic circuit is disclosed. The hydraulic circuit may comprise an actuation valve configured to actuate a flow of hydraulic fluid to and from the hydraulic consumer, a first control valve in fluid communication with the actuation valve through a first pilot line and configured to displace the actuation valve to a first position when actuated, and a second control valve in fluid communication with the actuation valve through a second pilot line and configured to displace the actuation valve to a second position when actuated. The first pilot line and the second line may each have a fixed minimum back pressure sufficient to maintain dissolved air in the hydraulic fluid.

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

Abstract: A telodendrimer-nanolipoprotein particle (t-NLP), comprising one or more membrane forming lipids, one or more telodendrimers, and a scaffold protein and a Chlamydia major outer membrane protein (MOMP) comprising a MOMP hydrophobic region, and related compositions methods and systems.

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

Abstract: A hydraulic circuit is disclosed. The hydraulic circuit may comprise an actuation valve configured to actuate a flow of hydraulic fluid to and from the hydraulic consumer, a first control valve in fluid communication with the actuation valve through a first pilot line and configured to displace the actuation valve to a first position when actuated, and a second control valve in fluid communication with the actuation valve through a second pilot line and configured to displace the actuation valve to a second position when actuated. The first pilot line and the second line may each have a fixed minimum back pressure sufficient to maintain dissolved air in the hydraulic fluid.

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing.

Abstract: A hydraulic system is disclosed. The hydraulic system may include a fluid source and an actuator having a first passage and a second passage. The hydraulic system may further include a pump having a first port connected to the first passage, a second port connected to the second passage, and a third port connected to the fluid source. The first and second passages may be connected to each other via the first and second ports, and the first passage and the low-pressure fluid source may be connected to each other via the first and third ports. They hydraulic system may further include a charge circuit fluidly connected to the first and second passages, and at least one damping control valve configured to selectively allow fluid from the pump to pass into the charge circuit to dampen pressure oscillations between the actuator and the pump.

Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.

Abstract: A hydraulic system is disclosed for use with a machine. The hydraulic system may have a closed-loop, meterless hydraulic circuit with a first pump fluidly connected to a first actuator. The hydraulic system may also have a closed-loop, metered hydraulic circuit with a second pump fluidly connected to a second actuator. The hydraulic system may further have a combiner valve configured to selectively direct fluid from the closed-loop metered hydraulic circuit to the closed-loop meterless hydraulic circuit.

Abstract: A machine including a first film dispenser which dispenses a first film, a second film dispenser which dispenses a second film, a first form system configured to form a first shape in the first film, a second form system configured to form a second shape in the second film, and a seal system configured to join the first film to the second film after the first shape is formed in the first film and the second shape is formed in the second film, wherein the first form system includes a bucket configured to hold a forming plate and a form head configured to form a cavity between the form head and the bucket that receives the first film.

Abstract: Intramedullary systems, expandable intramedullary nails, expandable anchors, and methods of using the same. The intramedullary system may include an expandable intramedullary nail configured to extend into an intramedullary canal of a long bone and/or one or more expandable anchors configured to extend at an angle transverse to the intramedullary nail. The intramedullary nails and/or anchors may include one or more integrated expansion mechanisms that allow for insertion in a contracted configuration and expansion into a deployed configuration to lock the relative position and prevent axial rotation and translation of the system.

Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.

Abstract: A system for auditing authorized key files associated with secure shell (SSH) servers is disclosed. In an example, the system may include a purpose-built SSH audit server. The SSH audit server may be configured to receive an authorized key file and a list of users. The SSH audit sever may generate and provide unique registration codes for each of the users in the list. The SSH audit server may associate particular users with particular public keys as each of the users accesses the SSH audit server using a public key and inputs a registration code.

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing.

Abstract: A system for auditing authorized key files associated with secure shell (SSH) servers is disclosed. In an example, the system may include a purpose-built SSH audit server. The SSH audit server may be configured to receive an authorized key file and a list of users. The SSH audit sever may generate and provide unique registration codes for each of the users in the list. The SSH audit server may associate particular users with particular public keys as each of the users accesses the SSH audit server using a public key and inputs a registration code.

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing.

Abstract: Methods and systems are provided for performing cathode calibration in a detector assembly. In one embodiment, a method comprises adjusting a cathode bias of a detector based on a total number of events occurring in the detector during a time period while maintaining an anode bias at a desired value, the events corresponding to photon energy detected by the detector. In this way, an automated cathode calibration procedure may be applied to calibrate the detector assembly.

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

Abstract: A hydraulic system is disclosed for assisting starting of a machine having an engine. The hydraulic system may include a work tool, a pump driven by the engine to pressurize fluid, and an actuator configured to receive pressurized fluid from the pump and move the work tool. The hydraulic system may also include an accumulator configured to selectively receive pressurized fluid from the pump and from the actuator, an electric starter configured to start the engine, and a motor selectively supplied with fluid from the accumulator to assist the electric starter in starting the engine.