Abstract: A solution for topical application to the skin for therapeutic and regenerative applications comprises about 0.05% by weight of annexin-1, about 0.76% by weight of galectin-1, about 0.23% by weight of protein S-100, about 0.003% by weight of timp-1, about 0.01% by weight of timp-2, about 0.01% by weight of ECM1, about 0.2% by weight of adiponectin, about 0.002% by weight of nephroblastoma overexpressed protein, about 0.0003% by weight of prostacyclin synthase, about 0.001% by weight of C-X-X motif chemokine, about 2% by weight of heparan sulphate, and about 0.01% by weight of apolipoprotein D.

Abstract: A method for diagnosing Parkinson's and Alzheimer's disease without invasive techniques or complicated wet-laboratory equipment. The method utilizes the rapid detection of both the CD38 protein and alpha-synuclein protein at a specific concentration that reflects the pathological state of Parkinson's disease, and CD38 protein and the tau protein which are found in significantly elevated concentrations in blood plasma and serum in the disease states. The blood plasma or serum can be collected and utilized as the test material for the pre- and post-symptom Parkinson's disease and Alzheimer's disease.

Abstract: A method of preparation of a solution used for therapeutic and regenerative applications having a concentration of at least about 0.05 microgram of total protein extract is disclosed. The method includes collecting an umbilical cord, incubating the umbilical cord in a solution containing antibiotics for sterilization, washing the umbilical cord in a buffered salt solution, homogenizing the umbilical cord in the buffered salt solution so as to produce an aqueous solution, applying an ultrasonic processer to the aqueous solution to sonicate and disrupt umbilical cord tissue, placing the aqueous solution into a centrifuge to separate the aqueous solution into a soluble component and a non-soluble component, filtrating and discarding the non-soluble component of the aqueous solution from the centrifuge through a 0.2 micron filter, and measuring protein concentration of the soluble component to insure at least about 0.05 microgram of total protein extract.

Abstract: A method and system for diagnosing concussions and other CNS afflictions is provided. A diagnostic kit includes a tube containing a predefined amount of lyophilized tau-specific antibody conjugated to colored latex nanoparticles, a fluid for mixing with the lyophilized tau-specific antibody conjugated to colored latex nanoparticles within the tube, so as to reconstitute the lyophilized tau-specific antibody conjugated to colored latex nanoparticles, resulting in a reconstituted mixture, and a swab comprising an absorbent hydrophobic material, the swab configured for absorbing cerebrospinal fluid when swabbed on a patient, wherein when the swab that has absorbed cerebrospinal fluid is placed in the tube containing the reconstituted mixture, the swab is configured to change color.

Abstract: A method of preparation of a solution used for therapeutic and regenerative applications having a concentration of at least about .05 microgram of total protein extract is disclosed. The method comprises the steps of: collecting an umbilical cord; incubating the umbilical cord in a solution containing antibiotics for sterilization; washing the umbilical cord in a buffered salt solution; homogenizing the umbilical cord in the buffered salt solution so as to produce an aqueous solution including the buffered salt solution; applying a sonicator at ultrasonic frequencies to the aqueous solution to further disrupt the aqueous solution; placing the aqueous solution into a centrifuge to separate the aqueous solution into a soluble component and a non-soluble component; filtrating and discarding the non-soluble component of the aqueous solution from the centrifuge through a 0.2 micron filter; and measuring protein concentration of the soluble component to insure at least about 0.05 microgram of total protein extract.

Abstract: A method and system for diagnosing concussions, TBI, CTE and other central nervous system disorders is provided. A diagnostic kit includes a tube containing a predefined amount of lyophilized biomarker-specific antibody conjugated to a conjugate with a color functionality, a fluid for mixing with the lyophilized biomarker-specific antibody conjugated to the conjugate within the tube, so as to reconstitute the lyophilized biomarker-specific antibody conjugated to the conjugate, resulting in a reconstituted mixture, and a swab comprising an absorbent hydrophobic material, the swab configured for absorbing cerebrospinal fluid when swabbed on a patient, wherein when the swab that has absorbed cerebrospinal fluid is placed in the tube containing the reconstituted mixture, the swab is configured to change color.

Abstract: A method and system for diagnosing concussions and other CNS afflictions is provided. A diagnostic kit includes a tube containing a predefined amount of lyophilized tau-specific antibody conjugated to colored latex nanoparticles, a fluid for mixing with the lyophilized tau-specific antibody conjugated to colored latex nanoparticles within the tube, so as to reconstitute the lyophilized tau-specific antibody conjugated to colored latex nanoparticles, resulting in a reconstituted mixture, and a swab comprising an absorbent hydrophobic material, the swab configured for absorbing cerebrospinal fluid when swabbed on a patient, wherein when the swab that has absorbed cerebrospinal fluid is placed in the tube containing the reconstituted mixture, the swab is configured to change color.