Abstract: A procoagulant designed to effect hemostasis at a puncture site in a patient's vasculature without the aid of manual compression is comprised of lyoplilized thrombin that is reconstituted with a buffered diluent and collagen that is mixed with the reconstituted thrombin to produce a viscous mixture. The buffered diluent is preferably of sodium phosphate and water. The procoagulant is essentially isotonic and has a pH in the range of 5-10. The method of making the procoagulant is comprised of the steps of reconstituting the lyophilized thrombin with the buffered diluent and then, mixing the reconstituted thrombin with collagen to both reduce the size of the particles of the collagen and form a fluid mixture.

Abstract: A method of assaying a sample of blood or blood components for the concentration of an immunophilin ligand comprising incubating the sample with a nonionic detergent and a protease, then inactivating the protease, and subsequently determining the concentration of the immunophilin ligand in the sample.