Abstract: A connected ecosystem for a laboratory environment comprises an electronic lab notebook, and instrumented biosafety cabinet, and one or more sensing vessels containing cell cultures. The electronic lab notebook interfaces with the instrumented biosafety cabinet to provide instructions, guidance, and monitoring of a user during the set up of the experimental protocol and to receive commands from the user via one of several input modalities. After the experimental protocol has been set up in the instrumented biosafety cabinet, cell cultures may be moved to an incubator where the connected ecosystem may provide automatic monitoring of the cultures. The automatic monitoring is provided by sensors integrated into cell culture vessels and supplemented by images of the cell cultures captured by a camera. The user may be informed of deviations from expected results detected based on the automatic monitoring.

Abstract: A cell culture bioreactor is disclosed that has a cell culture vessel with an interior reservoir to contain a cell substrate, an inlet fluidly connected to the reservoir, and an outlet fluidly connect to the reservoir. The bioreactor also has a sensor system for measuring impedance within the reservoir. The sensor system includes a pair of electrodes that are spaced apart from each other with at least a portion of the cell substrate disposed between the pair of electrodes. Each electrode of the pair of electrodes includes a plurality of pores to allow at least one of cells and culture media to flow through the electrode.

Abstract: A cell monitoring plate comprises a flat surface on which multiple cell culturing vessels may be stacked. The flats surface has multiple optical imaging systems embedded therein to fully image a cell culture vessels stacked on the plate. Each one of the multiple optical imaging systems provides both illumination and imaging through a single aperture in the surface of the monitoring plate.

Abstract: Methods, systems, and devices for non-invasive measurement of cell cultures are described that provide for remote monitoring of cell status. The system includes a cell culture vessel, at least one monitoring layer, at least one measurement device, and a communication component. The cell culture vessel may include at least one cell culture chamber configured for cell growth and for closed-system operation. The monitoring layer is external to the at least one cell culture chamber. In some cases, the communication component is configured to transmit data from the monitoring layer to a remote location.

Abstract: A connected ecosystem for a laboratory environment comprises an electronic lab notebook, and instrumented biosafety cabinet, and one or more sensing vessels containing cell cultures. The electronic lab notebook interfaces with the instrumented biosafety cabinet to provide instructions, guidance, and monitoring of a user during the set up of the experimental protocol and to receive commands from the user via one of several input modalities. After the experimental protocol has been set up in the instrumented biosafety cabinet, cell cultures may be moved to an incubator where the connected ecosystem may provide automatic monitoring of the cultures. The automatic monitoring is provided by sensors integrated into cell culture vessels and supplemented by images of the cell cultures captured by a camera. The user may be informed of deviations from expected results detected based on the automatic monitoring.

Abstract: A cell monitoring plate comprises a flat surface on which multiple cell culturing vessels may be stacked. The flats surface has multiple optical imaging systems embedded therein to fully image a cell culture vessels stacked on the plate. Each one of the multiple optical imaging systems provides both illumination and imaging through a single aperture in the surface of the monitoring plate.

Abstract: A cell monitoring plate comprises a flat surface on which multiple cell culturing vessels may be stacked. The flats surface has multiple optical imaging systems embedded therein to fully image a cell culture vessels stacked on the plate. Each one of the multiple optical imaging systems provides both illumination and imaging through a single aperture in the surface of the monitoring plate.

Abstract: Methods, systems, and devices for non-invasive measurement of cell cultures are described that provide for remote monitoring of cell status. The system includes a cell culture vessel, at least one monitoring layer, at least one measurement device, and a communication component. The cell culture vessel may include at least one cell culture chamber configured for cell growth and for closed-system operation. The monitoring layer is external to the at least one cell culture chamber. In some cases, the communication component is configured to transmit data from the monitoring layer to a remote location.

Abstract: A flask for the culturing of cells is disclosed. The cell culture chamber is defined by top and bottom walls connected by side and end walls, one end wall shaped such that media can drain to a bottommost spot thereby allowing for the complete removal of media via a vertically oriented pipette inserted into the interior of the flask body through an open neck.

Abstract: Thermoplastic polymeric sheets are rendered microporous and remain substantially flat by contacting the sheet with a first fluid composition that contains or more solvents for the polymeric sheet to render the sheet microporous and then contacting the microporous sheet with a second fluid composition that is substantially free of solvents for the polymer and that contains a non-solvent that is miscible with the one or more solvents of the first composition. Contacting the microporous sheet with the second fluid composition preferably occurs prior to substantial evaporation of the first fluid compositions, or solvents thereof, from the microporous sheet.