Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

Abstract: A hydraulic system is disclosed for assisting starting of a machine having an engine. The hydraulic system may include a work tool, a pump driven by the engine to pressurize fluid, and an actuator configured to receive pressurized fluid from the pump and move the work tool. The hydraulic system may also include an accumulator configured to selectively receive pressurized fluid from the pump and from the actuator, an electric starter configured to start the engine, and a motor selectively supplied with fluid from the accumulator to assist the electric starter in starting the engine.

Abstract: A method for making a coating composition by mixing one or more latex polymers and an aliphatic epoxy resin system having an epoxy equivalent weight less than 1000 and being distinct from the one or more latex polymers to provide an aqueous first component, and providing a second component having reactive groups that can react with the one or more epoxy resins. A mixture of the first and second components provides a film-forming curable coating composition that may be used on substrates including cement, cement fiberboard, wood, metal, plastic, ceramic, glass and composites.

Abstract: A hydraulic system is disclosed for use with a machine. The hydraulic system may have a closed-loop, meterless hydraulic circuit with a first pump fluidly connected to a first actuator. The hydraulic system may also have a closed-loop, metered hydraulic circuit with a second pump fluidly connected to a second actuator. The hydraulic system may further have a combiner valve configured to selectively direct fluid from the closed-loop metered hydraulic circuit to the closed-loop meterless hydraulic circuit.

Abstract: An implement system is disclosed for use with a machine having an engine. The implement system may have a pump driven by the engine, a first actuator configured to receive pressurized fluid from the pump during operation of the engine, and at least a second actuator configure to receive pressurized fluid from the pump during operation of the engine. The implement system may also have an accumulator configured to receive pressurized fluid from the first actuator during operation of the engine, and to discharge fluid to the first actuator and to the at least a second actuator when the engine is shut down.

Abstract: A method for making a coating composition by mixing one or more latex polymers and an aliphatic epoxy resin system having an epoxy equivalent weight less than 1000 and being distinct from the one or more latex polymers to provide an aqueous first component, and providing a second component having reactive groups that can react with the one or more epoxy resins. A mixture of the first and second components provides a film-forming curable coating composition that may be used on substrates including cement, cement fiberboard, wood, metal, plastic, ceramic, glass and composites.

Abstract: Methods and systems are provided for performing cathode calibration in a detector assembly. In one embodiment, a method comprises adjusting a cathode bias of a detector based on a total number of events occurring in the detector during a time period while maintaining an anode bias at a desired value, the events corresponding to photon energy detected by the detector. In this way, an automated cathode calibration procedure may be applied to calibrate the detector assembly.

Abstract: A hydraulic system is disclosed. The hydraulic system may include a fluid source and an actuator having a first passage and a second passage. The hydraulic system may further include a pump having a first port connected to the first passage, a second port connected to the second passage, and a third port connected to the fluid source. The first and second passages may be connected to each other via the first and second ports, and the first passage and the low-pressure fluid source may be connected to each other via the first and third ports. They hydraulic system may further include a charge circuit fluidly connected to the first and second passages, and at least one damping control valve configured to selectively allow fluid from the pump to pass into the charge circuit to dampen pressure oscillations between the actuator and the pump.

Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.

Abstract: A cylinder-piston assembly includes a cylinder barrel having a first end and a second end. An end cap is coupled to the cylinder barrel at the first end having a cylindrical opening. A piston slidably disposed in the cylinder barrel has a piston rod connected to the piston. The piston rod passes through the cylindrical opening of the end cap. A spring is disposed circumferentially around the piston rod. A load cell disposed in the end cap is adapted to generate a signal indicative of the cylinder displacement based on a spring force exerted on the load cell during a movement of the piston in the cylinder barrel, wherein the load cell is pressure balanced to minimize effect of forces other than the spring force exerted on the load cell.

Abstract: Disclosed are rapid and reproducible methods for separating and purifying nucleic acids from paraffin-containing tissue samples. The disclosed methods involve a melting step, where paraffin-containing tissue samples are heated in the presence of a detergent, causing the paraffin to melt and tissue sample cells to lyse. From the resulting two-phase mixture (i.e., a paraffin phase and an aqueous phase), the aqueous phase is collected for purification of nucleic acid. Protease(s) can be used at one or more points in the separation process to facilitate tissue cell lysis and/or degrade proteins that could degrade nucleic acid or interfere with subsequent genetic manipulation or analysis.

Abstract: A system for auditing authorized key files associated with secure shell (SSH) servers is disclosed. In an example, the system may include a purpose-built SSH audit server. The SSH audit server may be configured to receive an authorized key file and a list of users. The SSH audit sever may generate and provide unique registration codes for each of the users in the list. The SSH audit server may associate particular users with particular public keys as each of the users accesses the SSH audit server using a public key and inputs a registration code.

Abstract: Methods and systems for isolating a high purity starch stream are provided. The methods provide an initial treatment step in advance of traditional grinding or fractionation operations (such as dry milling or wet milling operations) that facilitate separation of starch from other components of starch-based grains, for example facilitate separation of soft endosperm from other components of corn kernels including hard endosperm. The systems include a first treatment system for separating a high purity starch stream from other components of the grain stream, wherein the system can be configured as a bolt on for traditional milling operations such as wet milling and dry milling operations.

Abstract: Methods, systems, and apparatuses for managing sensor information are disclosed. An example apparatus can comprise a power unit configured to collect energy, a sensor unit configured to receive sensor data from a sensor, a memory unit configured to store the sensor data, a communication unit configured to wirelessly broadcast the sensor data to one or more sensor nodes in a network of sensor nodes, and a processing unit. The processing unit can be configured to operate at least one of the sensor unit, the memory unit, and the communication unit after the energy collected in the power unit reaches a threshold value.

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

Abstract: A method is provided for controlling a hydraulic system having at least an implement pump, a fan-steering-braking (FSB) pump, a valve assembly, a fan drive system and one or more accumulators. The method may determine an operational state of a power source associated with the hydraulic system, actuate one or more valves of the valve assembly such that the FSB pump charges the accumulators and operates at least the fan drive system if the power source is operating at less than maximum power, and actuate one or more valves of the valve assembly such that the accumulators operate at least the fan drive system and such that the FSB pump supplements the implement pump as needed if the power source is operating at maximum power.

Abstract: A pultrusion process comprises steps of: pulling a matrix of continuous reinforcement fibers through a wet-out chamber and forming die while injecting thermoplastic resin (e.g. thermoplastic polyurethane) into the wet-out chamber, and providing secondary stations that remove, reform, and/or add material downstream of the forming die using heat and pressure to form a final beam shape with integrally-formed features thereon. The reinforcement matrix is optimally glass fiber, but can include different types of reinforcements, including strands, bundles, mats, weavings, rods, and other reinforcements and combinations of reinforcements, each of which can be strategically located in the beam profile. The final beam includes intermittent or continuous specialized features integrated into the beam along its length, allowing reduction of material waste, reduced/minimized later assembly, and reduced total number of parts.

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.

Abstract: A hydraulic system is disclosed for assisting starting of a machine having an engine. The hydraulic system may include a work tool, a pump driven by the engine to pressurize fluid, and an actuator configured to receive pressurized fluid from the pump and move the work tool. The hydraulic system may also include an accumulator configured to selectively receive pressurized fluid from the pump and from the actuator, an electric starter configured to start the engine, and a motor selectively supplied with fluid from the accumulator to assist the electric starter in starting the engine.