Abstract: The present invention provides a resist substrate treating solution and a method employing the solution for treating a resist substrate. This treating solution enables to remove efficiently resist residues remaining on a surface of the resist substrate after development, and further to miniaturize a resist pattern. The solution is used for treating a resist substrate having a developed photoresist pattern, and comprises a solvent incapable of dissolving the photoresist pattern and a polymer soluble in the solvent. The developed resist substrate is brought into contact with the treating solution, and then washed with a rinse solution such as water to remove efficiently resist residues remaining on the resist substrate surface. The solvent and the polymer are preferably water and a water-soluble polymer, respectively.

Abstract: It is intended to provide a method for easily detecting a phosphorylated peptide (protein) in a test sample by using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) which has been conventionally employed in analyzing a protein; a polyacrylamide gel for electrophoresis to be used in the method; a method of producing the gel; and a synthesizing intermediate in producing the gel. The polyacrylamide gel for electrophoresis to be used in the method of the invention is characterized in that at least a part of the structure thereof has a structure represented by the following formula (I); wherein M2+represents a transition metal ion; and X represents a linker group.

Abstract: The present invention provides a method for measuring a surface plasmon resonance, the method enabling easy detection of the existence of a phosphorylated peptide (protein) and determination whether a peptide is phosphorylated or not in biological materials. The present invention also provides a noble metal compound having high capability of coordination to a phosphorylated peptide, and being conveniently usable in the method. A first method for measuring surface plasmon resonance of the present invention comprises: placing a noble metal compound on a bottom face of a prism, irradiating a light to the prism to detect a reflected light, wherein, the noble metal compound has substituents of following formula (I) on a side opposite to a side contacting the prism, and a subject sample is added to a side having the substituent groups (I) in the noble metal compound.

Abstract: It is intended to provide a method for easily detecting a phosphopeptide (protein) in a test sample by using SDS-poly-acrylamide gel electrophoresis (SDS-PAGE) which has been employed in analyzing a protein, a polyacrylamide gel for electrophoresis to be used in the above method, a method of producing the gel and an intermediate in synthesizing the gel. The polyacrylamide gel for electrophoresis to be used in the above method has a structure represented by the following general formula (I) in at least a part of the structure thereof: (I) wherein M2+ represents a transition metal ion; and X represents a linker.

Abstract: It is intended to provide a method by which a substance having a phosphate group can be easily detected from a biological sample or the like, a method by which a phosphorylated amino acid residue is easily identified and a compound which can be used in the methods because of having a high coordinate bond forming capacity with a substance having a phosphate group.

Abstract: The present invention provides a method for measuring a surface plasmon resonance, the method enabling easy detection of the existence of a phosphorylated peptide (protein) and determination whether a peptide is phosphorylated or not in biological materials. The present invention also provides a noble metal compound having high capability of coordination to a phosphorylated peptide, and being conveniently usable in the method. A first method for measuring surface plasmon resonance of the present invention comprises: placing a noble metal compound on a bottom face of a prism, irradiating a light to the prism to detect a reflected light, wherein, the noble metal compound has substituents of following formula (I) on a side opposite to a side contacting the prism, and a subject sample is added to a side having the substituent groups (I) in the noble metal compound.

Abstract: A method for capturing a substance having an anionic substituent by contacting a substance having an anionic substituent with a zinc complex such that the substance binds to the zinc complex, the zinc complex being represented by a formula (I): wherein each R is the same or different from each other and each R represents H; an alkyl group having 1 to 16 carbon atoms; an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group, wherein an alkyl portion of the acyl group, the carboxyalkyl group, the acylalkyl group, the carbamoylalkyl group, the cyanoalkyl group, the hydroxyalkyl group, the aminoalkyl group and the haloalkyl group has 1 to 16 carbon atoms; a carboxyl group; a carbamoyl group; a hydroxyl group; a cyano group; an amino group; or a halogeno group.

Abstract: The present invention provides a method for confirming existence of a phosphoric acid monoester compound (peptide, saccharine and the like) and easily identifying the molecular weight thereof even among biological samples including a plurality of compounds, and an additive for a mass spectrometry used for the method. In the method according to the present invention, a complex compound exhibiting extremely high coordination ability to a phosphoric acid monoester group and configured of single kind of zinc isotopes is used to obtain a plurality of mass spectrum data, and then the data are compared.

Abstract: A polymer carrier to which a prescribed zinc complex group is binding directly or through a spacer, having property to bond, under a certain condition, to an anionic substituent (a phosphate group, for example), showing low solubility to a solvent (preferably insolubility to a solvent) as a whole, and being capable of capturing, separating and purifying easily a substance having an anionic substituent (a phosphate group, for example).

Abstract: The present invention is directed to a phosphate ion adsorbent comprising a polymer or a salt thereof with a metal complex group represented by the following general formula (II): bound thereto directly or through a spacer. The phosphate ion adsorbent of the present invention is advantageous not only in that the adsorbent specifically adsorbs phosphate ions in a living body, but also in that the adsorbent has high adsorbability to phosphate ions, thus useful in removing the phosphate ions from the living body.

Abstract: A method for capturing a substance having an anionic substituent by contacting a substance having an anionic substituent with a zinc complex such that the substance binds to the zinc complex, the zinc complex being represented by a formula (I): wherein each R is the same or different from each other and each R represents H; an alkyl group having 1 to 16 carbon atoms; an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group, wherein an alkyl portion of the acyl group, the carboxyalkyl group, the acylalkyl group, the carbamoylalkyl group, the cyanoalkyl group, the hydroxyalkyl group, the aminoalkyl group and the haloalkyl group has 1 to 16 carbon atoms; a carboxyl group; a carbamoyl group; a hydroxyl group; a cyano group; an amino group; or a halogeno group.

Abstract: A zinc complex represented by formula (I-0): {wherein R may be the same or different from each other and represents H; an alkyl group having 1 to 16 carbon atoms; an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group (here, a carbon number at an alkyl portion of these groups is 1 to 16); a carboxyl group; a carbamoyl group; a hydroxyl group; a cyano group; an amino group or; a halogeno group (provided that all are Hs are excluded)} which is capable of capturing a substance having an anionic substituent.

Abstract: Provided are a method for easily detecting phosphorylated peptides, namely, proteins, in samples derived from living organisms or the like, a method for selectively adsorbing the phosphorylated peptides, and compounds that are highly coordinated to the phosphorylated peptides and usable in the methods. The complex compound is represented by the formula: wherein X is a linker moiety, and Y is a labeling group. The compound (I) is highly coordinated to a phosphorylated peptide, and has a labeling group. Accordingly, with use of the compound (I), the phosphorylated peptide can be easily identified.

Abstract: The present invention provides a method for confirming existence of a phosphoric acid monoester compound (peptide, saccharine and the like) and easily identifying the molecular weight thereof even among biological samples including a plurality of compounds, and an additive for a mass spectrometry used for the method. In the method according to the present invention, a complex compound exhibiting extremely high coordination ability to a phosphoric acid monoester group and configured of single kind of zinc isotopes is used to obtain a plurality of mass spectrum data, and then the data are compared.

Abstract: A polymer carrier to which a prescribed zinc complex group is binding directly or through a spacer, having property to bond, under a certain condition, to an anionic substituent (a phosphate group, for example), showing low solubility to a solvent (preferably insolubility to a solvent) as a whole, and being capable of capturing, separating and purifying easily a substance having an anionic substituent (a phosphate group, for example).

Abstract: A zinc complex represented by formula (I-0): {wherein R may be the same or different from each other and represents H; an alkyl group having 1 to 16 carbon atoms; an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group (here, a carbon number at an alkyl portion of these groups is 1 to 16); a carboxyl group; a carbamoyl group; a hydroxyl group; a cyano group; an amino group or; a halogeno group (provided that all are Hs are excluded)} which is capable of capturing a substance having an anionic substituent.

Abstract: Provided are a method for easily detecting phosphorylated peptides, namely, proteins, in samples derived from living organisms or the like, a method for selectively adsorbing the phosphorylated peptides, and compounds that are highly coordinated to the phosphorylated peptides and usable in the methods.

Abstract: Disclosed is a scan path circuit for testing a logic circuit, which comprises a plurality of scan cells each having a scan in SI, a cell output and a clock input for receiving a clock signal, connected in series with respect to the scan ins and cell outputs. Each scan cell includes a scan flip-flop 21, and a selection circuit 31 which selects either a signal of a scan in SI or a signal of a scan out SO of the scan flip-flop 21 according on a selection controlling signal to provide the selected signal to the cell output. Determining the values of the selector controlling signal with a bypass controlling shift resister 45 permits forming a bypass between the scan data input terminal SDI and the scan in SI of any scan flip-flop except the first stage flip-flop, and/or a bypass between the scan data output terminal SDO and the scan out SO of any scan flip-flop except the final stage scan flip-flop.