Abstract: A method for producing a liquid reaction mixture containing a radioisotope, in particular, a radioactive composition, minimizes device contamination with radioactive substances and increase speed and accuracy with which droplets are mixed. The method for producing a radioactive composition includes placing at least one first droplet L1 containing a radionuclide and at least one second droplet L2 containing a labeling substance on at least two respective dimples 5 among dimples 5 on a front surface 4b of an insulating layer 4 of a liquid manipulation device 1, and obtaining a liquid mixture M by using a change in electrostatic force caused by changing voltage applied to the electrodes 3 to thereby cause a relative movement between the at least one first droplet L1 and the at least one second droplet L2 so that the at least one first droplet L1 and the at least one second droplet L2 are mixed together at any one dimple among the dimples 5.

Abstract: An open-type liquid manipulation device can divide liquid, in particular, a droplet efficiently. The open-type liquid manipulation device according to the present invention includes: a substrate 1, 11, 21; at least three electrodes 2, 12, 13, 22, 23 located on a front surface 1b, 11b, 21b of the substrate 1, 11, 21; and an insulating layer 3, 14, 24 located over the front surface 1b, 11b, 21b of the substrate 1, 11, 21 to cover the at least three electrodes 2, 12, 13, 22, 23. The device includes a groove 4, 15, 25 that is concave in a direction from a front surface 3b, 14b, 24b of the insulating layer 3, 14, 24 toward a back surface 3a, 14a, 24a of the insulating layer 3, 14, 24. The groove 4, 15, 25 extends straddling the at least three electrodes. Liquid L is controlled on the front surface 3b, 14b, 24b of the insulating layer 3, 14, 24 by using a change in electrostatic force generated by changing voltage applied to the electrodes 2, 12, 13, 22, 23.

Abstract: A liquid manipulation device has an enhanced ability to control liquid, in particular, a droplet, and offers improved fabrication efficiency. The liquid manipulation device according to the present invention includes: a substrate 1, 11 including a sheet shape or a film shape to have flexibility; a plurality of electrodes 2 located on a front surface 1b, 11b of the substrate 1, 11; and an insulating layer located over the front surface 1b, 11b of the substrate 1, 11 to cover the electrodes 2. The liquid manipulation device is configured to move liquid L on a front surface 3b of the insulating layer 3 by using an electrostatic force that is generated when voltage is applied to at least one of the electrodes 2. In the liquid manipulation device, the insulating layer 3 includes dimples 4 that are located in correspondence with the electrodes 2 and are curved concave in a concave direction directed from the front surface 3b of the insulating layer 3 toward the back surface 3a of the insulating layer 3.

Abstract: A method for producing a liquid reaction mixture containing a radioisotope, in particular, a radioactive composition, minimizes device contamination with radioactive substances and increase speed and accuracy with which droplets are mixed. The method for producing a radioactive composition includes placing at least one first droplet L1 containing a radionuclide and at least one second droplet L2 containing a labeling substance on at least two respective dimples 5 among dimples 5 on a front surface 4b of an insulating layer 4 of a liquid manipulation device 1, and obtaining a liquid mixture M by using a change in electrostatic force caused by changing voltage applied to the electrodes 3 to thereby cause a relative movement between the at least one first droplet L1 and the at least one second droplet L2 so that the at least one first droplet L1 and the at least one second droplet L2 are mixed together at any one dimple among the dimples 5.

Abstract: A protocol created in such a format that a series of operations in cell culture are executable by a robot 10 is acquired (S1). The robot 10 is controlled to implement the operations according to the protocol (S2). In order to modify the protocol after the implementation of the operations, modification information on at least one action among basic actions which serve as bases for implementing the operations and is performed on an instrument used by the robot 10 in the operations, and complementary actions which complement the basic actions is acquired (S5). The robot 10 is controlled to produce cells by using the protocol modified based on the modification information (S7).

Abstract: A protocol created in such a format that a series of operations for process targets in the fields of engineering related to living organisms are executable by a robot 10 is acquired (S1). The robot 10 is controlled to implement the operations for the process targets according to the protocol (S2). In order to modify the protocol after the implementation of the operations, modification information on at least one action among basic actions which serve as bases for implementing the operations and is performed on an instrument used by the robot 10 in the operations, and complementary actions which complement the basic actions is acquired (S5). The robot 10 is controlled to produce processed products from the process targets by using the protocol modified based on the modification information (S7).

Abstract: It is an object of the present invention to provide an antitumor agent, which is further excellent as a treatment agent used in the prevention and/or therapy of tumor associated with a bromodomain, and also to provide a bromodomain inhibitor, which is useful as a treatment agent for diseases or states associated with a bromodomain. An antitumor agent and a bromodomain inhibitor, comprising a compound represented by the following formula, have an excellent bromodomain inhibitory activity and are useful as treatment agents in the prevention and/or therapy of tumor associated with a bromodomain, and the like: wherein R1 represents a C1-6 alkyl group, etc.; R2 represents a hydrogen atom, etc.; R3 represents a halogen atom, etc.; Z1, Z2 and Z3 each represent CH, etc.; X1 represents CONH, etc.; Ring A represents a phenyl group, etc.; R4 represents a halogen atom, etc.; and m represents an integer from 0 to 5.

Abstract: It is an object of the present invention to provide an antitumor agent, which is further excellent as a treatment agent used in the prevention and/or therapy of tumor associated with a bromodomain, and also to provide a bromodomain inhibitor, which is useful as a treatment agent for diseases or states associated with a bromodomain. An antitumor agent and a bromodomain inhibitor, comprising a compound represented by the following formula, have an excellent bromodomain inhibitory activity and are useful as treatment agents in the prevention and/or therapy of tumor associated with a bromodomain, and the like: wherein R1 represents a C1-6 alkyl group, etc.; R2 represents a hydrogen atom, etc.; R3 represents a halogen atom, etc.; Z1, Z2 and Z3 each represent CH, etc.; X1 represents CONH, etc.; Ring A represents a phenyl group, etc.; R4 represents a halogen atom, etc.; and m represents an integer from 0 to 5.

Abstract: Provided is a pipette device that can discharge or withdraw a liquid easily by liquid processing operation by a robot device. Provided is a pipette device held by a robot device, comprising a driving part for withdrawing and/or discharging a liquid, a signal receiving part for receiving a signal from the robot device and a control part for controlling the driving part according to the signal.

Abstract: The present invention provides a water-cooled heat sink having an integrated structure, and having inside a precise, freely formed flow path, the water-cooled heat sink being produced without using a technique for pasting a plurality of metal sheets. In a water-cooled heat sink 1, a formed body 2 is provided outwardly, a flow path 3 for passage of a liquid is formed inwardly of the formed body 2, and an inlet 5 and an outlet 4 for the liquid, which communicate with the flow path 3, are formed in the main body of the heat sink 1. The entire formed body 2 of the heat sink 1 is integrally formed by thick coating layers applied by electroplating, and has no joints.

Abstract: The present invention provides a water-cooled heat sink having an integrated structure, and having inside a precise, freely formed flow path, the water-cooled heat sink being produced without using a technique for pasting a plurality of metal sheets. In a water-cooled heat sink 1, a formed body 2 is provided outwardly, a flow path 3 for passage of a liquid is formed inwardly of the formed body 2, and an inlet 5 and an outlet 4 for the liquid, which communicate with the flow path 3, are formed in the main body of the heat sink 1. The entire formed body 2 of the heat sink 1 is integrally formed by thick coating layers applied by electroplating, and has no joints.

Abstract: Disclosed is a labeling substance capable of reversibly immobilizing (reversible detachment) to a solid surface and to reliably and accurately identify a target biological substance interacting with a probe substance bound to the labeling substance. A chimeric compound is provided, which includes a labeling substance L including (1) an organic compound S with a chemical structure capable of binding to a probe substance P, which can interact with a biological substance B and (2) a peptide bound to the organic compound S and specifically recognized by an antibody A, peptide and a probe substance P at least except a peptide or protein. In addition, a method for preparing the labeling substance L and a method for capturing, structurally analyzing and/or identifying the biological substance using the labeling substance L are provided.

Abstract: A method for screening therapeutic agents for disuse muscular atrophy is provided that includes the steps of interacting a selected protein with a polyubiquitin chain in the presence of a candidate therapeutic agent, and determining the effect of the candidate on the binding strength between the protein and the polyubiquitin chain. The effect may be observed by color development on a substrate when the steps are carried out by way of an enzyme-linked immunosorbent assay, or by direct observation via NMR spectroscopy, X-ray crystal analysis, electron microscopy or surface plasmon resonance.

Abstract: The invention aims to provide a highly accurate automatic biopolymer determination technique utilizing mass spectrometry whereby calibration prior to measurement or the addition of an internal standard to a sample can be eliminated. The biopolymer automatic identifying method of the invention comprises: retrieving a candidate molecule by matching an observed mass value X obtained by mass spectrometry with a predetermined database; selecting an arbitrary number of candidate molecules with high similarity scores; calibrating the observed mass value X using the candidate molecule as an internal standard; calculating relative error Ec between a calibrated mass value Xc and a theoretical mass value M of the candidate molecule; determining the standard deviation SEc of the relative error; determining a tolerance Tc of database search from the standard deviation SEc; and repeating a database search based on the tolerance Tc.

Abstract: The present invention provides a water-cooled heat sink having an integrated structure, and having inside a precise, freely formed flow path, the water-cooled heat sink being produced without using a technique for pasting a plurality of metal sheets. In a water-cooled heat sink 1, a formed body 2 is provided outwardly, a flow path 3 for passage of a liquid is formed inwardly of the formed body 2, and an inlet 5 and an outlet 4 for the liquid, which communicate with the flow path 3, are formed in the main body of the heat sink 1. The entire formed body 2 of the heat sink 1 is integrally formed by thick coating layers applied by electroplating, and has no joints.

Abstract: [Problem] Providing a novel interaction between proteins. [Solution Means] It has been found that there is an interaction between proteins between proteasome and ZNF216 (or AWP1). It has also been found that there is an interaction between proteins between a polyubiquitin chain and ZNF216 (or AWP1). By utilizing this novel interaction between the proteins, a therapeutic agent for disuse muscular atrophy, and a method for screening the therapeutic agents for disuse muscular atrophy, a marker for disease diagnosis, a method for evaluating the risk of onset of the disuse muscular atrophy, etc., are provided.

Abstract: [Problem] To provide a labeling substance capable of reversibly immobilizing (reversible detachment) to a solid surface and to reliably and accurately identify a target biological substance interacting with a probe substance bound to the labeling substance. [Solving Means] A chimeric compound is provided, which includes a labeling substance L including (1) an organic compounds with a chemical structure capable of binding to a probe substance P, which can interact with a biological substance B and (2) a peptide bound to the organic compound S and specifically recognized by an antibody A, peptide and a probe substance P at least except a peptide or protein. In addition, a method for preparing the labeling substance L and a method for capturing, structurally analyzing and/or identifying the biological substance using the labeling substance L are provided.

Abstract: The present invention aims to provide a liquid chromatograph, an analysis system, and a gradient elution method, which are designed to permit a gradient change of eluent with higher precision and also permit a high sensitive detection or analysis.

Abstract: The invention aims to provide a highly accurate automatic biopolymer determination technique utilizing mass spectrometry whereby calibration prior to measurement or the addition of an internal standard to a sample can be eliminated. The biopolymer automatic identifying method of the invention comprises: retrieving a candidate molecule by matching an observed mass value X obtained by mass spectrometry with a predetermined database; selecting an arbitrary number of candidate molecules with high similarity scores; calibrating the observed mass value X using the candidate molecule as an internal standard; calculating relative error Ec between a calibrated mass value Xc and a theoretical mass value M of the candidate molecule; determining the standard deviation SEc of the relative error; determining a tolerance Tc of database search from the standard deviation SEc; and repeating a database search based on the tolerance Tc.

Abstract: A problem is to provide a microspray column capable of increasing the ionization efficiency, and a high sensitive mass spectrometer and a mass spectrometric method using such a microspray column. For solving this problem, we provide a column for introducing a sample in an ionization source of a mass spectrometer (ESI/MS) being designed to ionize a sample molecule with electrospray and introduce it into an analyzer, has structural features of: (1) 0.5 ?m or less in an inner diameter of a tip opening of the column; (2) 0.5 ?m or more and 5 ?m or less in a particle size of a column filler; and (3) fritless, a microspray column having the above (1) to (3), a mass spectrometer having such a microspray column in an ionization source, and a mass spectrometric method capable of performing a nonoflow electrospray with the microspray column.