Abstract: The present disclosure describes a method and system for monitoring the bandwidth utilization on a high speed data network port over time. According to the method described herein, measurements of the bandwidth utilization on the port may be taken over a predetermined time interval and analyzed to determine whether the utilization measurement equals or exceeds a predetermined upper threshold. If the port exhibits high bandwidth utilization within the time interval, an alert may be set for further monitoring of the port or for port augmentation.

Abstract: This invention relates to methods for variant cytosine detection, and kits and probes for variant cytosine detection. In particular the variant cytosine detection is related to detection of methylated cytosine, hydroxymethylated cytosine, carboxycytosine and/or formylcytosine in nucleic acid.

Abstract: Cache management techniques are described for a content distribution network (CDN), for example, a video on demand (VOD) system supporting user requests and delivery of video content. A preferred cache size may be calculated for one or more cache devices in the CDN, for example, based on a maximum cache memory size, a bandwidth availability associated with the CDN, and a title dispersion calculation determined by the user requests within the CDN. After establishing the cache with a set of assets (e.g., video content), an asset replacement algorithm may be executed at one or more cache devices in the CDN. When a determination is made that a new asset should be added to a full cache, a multi-factor comparative analysis may be performed on the assets currently residing in the cache, comparing the popularity and size of assets and combinations of assets, along with other factors to determine which assets should be replaced in the cache device.

Abstract: Oligonucleotide chemistry is central to the advancement of core technologies such as DNA sequencing, forensic and genetic analysis and has impacted greatly on the discipline of molecular biology. Oligonucleotides and their analogues are essential tools in these areas. They are often produced by automated solid-phase phosphoramidite synthesis but it is difficult to synthesize long DNA and RNA sequences by this method. Methods are proposed for ligating oligonucleotides together, in particular the use of an azide-alkyne coupling reaction to ligate the backbones of oligonucleotides together to form longer oligonucleotides that can be synthesized using current phosphoramidite synthesis methods.

Abstract: The present disclosure describes a method and system for monitoring the bandwidth utilization on a high speed data network port over time. According to the method described herein, measurements of the bandwidth utilization on the port may be taken over a predetermined time interval and analyzed to determine whether the utilization measurement equals or exceeds a predetermined upper threshold. If the port exhibits high bandwidth utilization within the time interval, an alert may be set for further monitoring of the port or for port augmentation.

Abstract: A device, system and method are provided to dynamically manage bandwidth for audiovisual communications and content distribution. The device, system and method may include dynamic qualification of content or channels for distribution in accordance with one or more distribution models, such as a broadcast distribution model and a narrowcast distribution model (e.g., a multicast distribution model and/or a unicast distribution model). In some embodiments, the qualification may be based on demand as reflected in requests for, or access to, content from user terminals. In some embodiments, based on changes in demand for content, a requalification of the content in terms of one or more distribution models may take place.

Abstract: A device, computer readable medium, system and method for overcoming bandwidth limitations is disclosed for determining that a bandwidth limitation is related to preventing delivery of content, identifying a version of the content capable of being transmitted over a lower bandwidth, querying a device requesting delivery of the content for an indication of acceptability of a lower bandwidth version of the content instead of a higher bandwidth version, and based on an affirmative response to the querying, causing delivery of the lower bandwidth version.

Abstract: Systems and methods are described that relate to on-demand compression for electronic data, such as media content, through a communication pathway. The communication pathway may be a configured to multicast media content to a plurality of end-users in a service group. In certain embodiments, the service group may comprise a service group node having a quadrature amplitude modulation device. In one exemplary method, it may be determined whether the amount of media content being transmitted meets a first utilization threshold. The determination may be based on, at least in part, by measurements taken at one or more electronic devices located throughout a network or system. If the first bandwidth utilization threshold is not met, media content may be transmitted in a first format. If the first bandwidth utilization threshold is met, at least a portion of the media content may be transmitted in a first optimized delivery technique.

Abstract: A device, system and method are provided to dynamically manage bandwidth for audiovisual communications and content distribution. The device, system and method may include dynamic qualification of content or channels for distribution in accordance with one or more distribution models, such as a broadcast distribution model and a narrowcast distribution model (e.g., a multicast distribution model and/or a unicast distribution model). In some embodiments, the qualification may be based on demand as reflected in requests for, or access to, content from user terminals. In some embodiments, based on changes in demand for content, a requalification of the content in terms of one or more distribution models may take place.

Abstract: A method for joining oligonucleotides. The method includes joining together one or more oligonucleotides by reacting an alkyne group lined to an oligonucleotide with an azide group linked to an oligonucleotide to form a triazole linkage. The alkyne group is a strained alkyne group. The method can include ligating together ends of one or more oligonucleotides or cross-linking strands of an oligonucleotide duplex. The methods described allow oligonucleotide strands to be ligated together without the need for a ligase enzyme.

Abstract: Methods and systems for providing content and content recommendations are described. Content recommendations for users in a service area may be based on content requests from users within the same service area. Content recommendations over a period of time may dynamically change based on many factors, including: the number of requests for particular content within one or more time frames, the storage location of requested content relative to requesting users, and whether a request occurs within a time period of rapid shift in use and types of content requested. A content recommendation including one or more content recommendations may be provided to users.

Abstract: Oligonucleotide chemistry is central to the advancement of core technologies such as DNA sequencing, forensic and genetic analysis and has impacted greatly on the discipline of molecular biology. Oligonucleotides and their analogues are essential tools in these areas. They are often produced by automated solid-phase phosphoramidite synthesis but it is difficult to synthesize long DNA and RNA sequences by this method. Methods are proposed for ligating oligonucleotides together, in particular the use of an azide-alkyne coupling reaction to ligate the backbones of oligonucleotides together to form longer oligonucleotides that can be synthesized using current phosphoramidite synthesis methods.

Abstract: Oligonucleotide chemistry is central to the advancement of core technologies such as DNA sequencing, forensic and genetic analysis and has impacted greatly on the discipline of molecular biology. Oligonucleotides and their analogues are essential tools in these areas. They are often produced by automated solid-phase phosphoramidite synthesis but it is difficult to synthesize long DNA and RNA sequences by this method. Methods are proposed for ligating oligonucleotides together, in particular the use of an azide-alkyne coupling reaction to ligate the backbones of oligonucleotides together to form longer oligonucleotides than can be synthesized using current phosphoramidite synthesis methods.

Abstract: A device, system and method are provided to dynamically manage bandwidth for audiovisual communications and content distribution. The device, system and method may include dynamic qualification of content or channels for distribution in accordance with one or more distribution models, such as a broadcast distribution model and a narrowcast distribution model (e.g., a multicast distribution model and/or a unicast distribution model). In some embodiments, the qualification may be based on demand as reflected in requests for, or access to, content from user terminals. In some embodiments, based on changes in demand for content, a requalification of the content in terms of one or more distribution models may take place.

Abstract: Cache management techniques are described for a content distribution network (CDN), for example, a video on demand (VOD) system supporting user requests and delivery of video content. A preferred cache size may be calculated for one or more cache devices in the CDN, for example, based on a maximum cache memory size, a bandwidth availability associated with the CDN, and a title dispersion calculation determined by the user requests within the CDN. After establishing the cache with a set of assets (e.g., video content), an asset replacement algorithm may be executed at one or more cache devices in the CDN. When a determination is made that a new asset should be added to a full cache, a multi-factor comparative analysis may be performed on the assets currently residing in the cache, comparing the popularity and size of assets and combinations of assets, along with other factors to determine which assets should be replaced in the cache device.

Abstract: An apparatus, system and method are disclosed for resolving bandwidth constriction using multiple bandwidths and/or multiple content delivery paths. The apparatus, system and method may include recording requested video content and/or delivering the recorded video content to resolve bandwidth constriction using alternate bandwidths or delivery paths.

Abstract: The present disclosure describes a method and system for monitoring the bandwidth utilization on a high speed data network port over time. According to the method described herein, measurements of the bandwidth utilization on the port may be taken over a predetermined time interval and analyzed to determine whether the utilization measurement equals or exceeds a predetermined upper threshold. If the port exhibits high bandwidth utilization within the time interval, an alert may be set for further monitoring of the port or for port augmentation.

Abstract: A method for detecting specific DNA sequences and discriminating single nucleotide polymorphisms (SNPs) using fluorescently labelled oligonucleotide probes is disclosed. Oligonucleotide probes are labelled with reporter molecules preferentially attached to an internal nucleotide residue. The fluorescence emission of oligonucleotide probes varies significantly when in single-stranded and double-stranded states despite the absence of quencher moieties, allowing reliable detection of complementary DNA targets. The melting temperature of probe/target duplexes permits discrimination of targets that differ by as little as a single nucleotide residue, such that polymorphic targets may be discriminated by fluorescence quantitation and Tm.

Abstract: Systems and methods for generating and receiving a sequenced listing of pieces of content are described. A request to search for assets may be received. A first listing of assets associated with the request may be received. Data representative of assets stored within an edge server associated with the user may be received. A first group of assets of the first listing of assets that are stored within the edge server based upon the received data representative of assets stored within the edge server and a second group of assets of the first listing of assets that are not stored within the edge server based upon the received data representative of assets stored within the edge server may be determined. A second listing of assets may be generated where at least one asset of the first group is sequenced separate from at least one asset of the second group.