Abstract: The producing method includes causing a cell suspension to flow into a tubular flow path; injecting a gel precursor into the flow path to cover the circumference of the cell suspension with the gel precursor; injecting a gas into the flow path to form, in the flow path, an alternating flow obtained from the gas and the cell suspension covered with the gel precursor; and injecting a gelating agent into the flow path and causing the gelating agent to join the alternating flow to the gel precursor.

Abstract: There is provided a method for easily determining an undifferentiated state of pluripotent stem cells without relying on the judgment of a skilled technician. The method includes: a step of evaluating an undifferentiated state of pluripotent stem cells based on a time-dependent change in a variation value of an extracellular metabolite contained in a culture medium in which the pluripotent stem cells are cultured, wherein the extracellular metabolite is at least one selected from a group consisting of L-glutamic acid, L-alanine and ammonia.

Abstract: A cell-holding container comprises: an elastic body for holding cells including at least any one of adherent cells at least one selected from the group consisting of stem cells, progenitor cells, somatic cells and germ cells, and suspended cells at least one selected from the group consisting of blood cells, T cells and B cells; and the elastic body is formed of a rubber material containing a rubber component including an additional crosslinking silicone rubber and is able to hold the cells.

Abstract: A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.

Abstract: A cell-holding container comprises: an elastic body for holding cells including at least any one of adherent cells at least one selected from the group consisting of stem cells, progenitor cells, somatic cells and germ cells, and suspended cells at least one selected from the group consisting of blood cells, T cells and B cells; and the elastic body is formed of a rubber material containing a rubber component including an additional crosslinking silicone rubber and is able to hold the cells.

Abstract: There is provided a method for easily determining an undifferentiated state of pluripotent stem cells without relying on the judgment of a skilled technician. The method includes: a step of evaluating an undifferentiated state of pluripotent stem cells based on a time-dependent change in a variation value of an extracellular metabolite contained in a culture medium in which the pluripotent stem cells are cultured, wherein the extracellular metabolite is at least one selected from a group consisting of L-glutamic acid, L-alanine and ammonia.

Abstract: A cell separation device imparts vibration to a cell culture film having a front surface to which cells adhere, from a rear surface side of the film, and consequently separates the cells from the film. The device includes an ultrasonic probe having an ultrasonically-vibrating vibrator, a frame disposed to surround the vibrator and a first detection part configured to detect a force applied to the frame in a specified direction. The vibrator selectively imparts ultrasonic vibration to specified cells adhering to the front surface of the film. Based on a detection result obtained by the first detection part, the frame is pushed until the force applied from the rear surface of the film to the frame reaches a specified magnitude. Thereafter, the vibrator ultrasonically vibrates in a state in which the frame makes contact with the rear surface of the film.

Abstract: A cell culture container characterized by being equipped with a container main body and a flat plate attached onto one surface of the container main body, wherein the container main body is equipped with an inflow port through which a liquid can flow into the container main body, a passage through which the liquid flowing into the container main body from the inflow port can pass, and an outflow port through which the liquid passing through the passage can flow out from the container main body, and wherein, on the bottom surface of the passage, multiple cell-seeding areas in which cells passing through the passage can be seeded are arranged side by side along the passage.

Abstract: The present invention provides a method of simply and uniformly subculturing pluripotent stem cells. The method includes the steps of dispersing cell masses obtained from passaging of the pluripotent stem cells into a single cell level, and subsequently, rapidly forming cell aggregates.

Abstract: A method for detaching cells from a cell culture surface includes irradiating visible light in an irradiation amount of 4 J or greater per 1 mm2 to a cell adhesion surface to which cells are adhering on a substrate such that the cells are detached from the cell adhesion surface on the substrate.