Abstract: A cloned DNA molecule is disclosed. The DNA molecule encodes a human endothelin-3 protein having the amino acid sequence: Glu-Gly-Ala-Pro-Glu-His-His-Arg-Ser-Arg-Arg-Cys-Thr-Cys-Phe-Thr-Tyr-Lys-As p-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp-Ile-Asn-Thr-Pro-Glu or any portion of said amino acid sequence containing Cys-Thr-Cys-Phe-Thr-Tyr-Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Il e-Ile-Trp. Also disclosed is a method of producing mature endothelin-3 protein. In the method, the host cell is cultured, allowing mature endothelin-3 to accumulate in the culture medium, and the mature endothelin-3 protein is separated from the culture medium.

Abstract: Disclosed are (1) a DNA sequence containing a DNA segment coding for endothelin-2 or human endothelin-3, (2) a precursor protein and a mature peptide of endothelin-2 or human endothelin-3, (3) a transformant carrying a DNA sequence containing a DNA segment coding for endothelin-2 or human endothelin-3 and (4) a method for preparing mature endothelin-2 or endothelin-3 which comprises culturing the transformant described in (3), producing and accumulating a protein in a culture medium, and collecting the same. Cells transfected or transformed with the DNA allow large amounts of endothelin-2 or endothelin-3 to be produced, which causes the advantageous production of endothelin-2 or endothelin-3. Endothelin-2 and endothelin-3 can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.

Abstract: Disclosed are (1) a DNA sequence containing a segment coding for endothelin-2 or human endothelin-3, (2) a precursor protein and a mature peptide of endothelin-2 or human endothelin-3, (3) a transformant carrying a DNA sequence containing a DNA segment coding for endothelin-2 or human endothelin-3 and (4) a method for preparing mature endothelin-2 or endothelin-3 which comprises culturing the transformant described in (3), producing and accumulating a protein in a culture medium, and collecting the same.Cells transfected or transformed with the DNA allow large amounts of endothelin-2 or endothelin-3 to be produced, which causes the advantageous production of endothelin-2 or endothelin-3.Endothelin-2 and endothelin-3 can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.

Abstract: Endothelin-2 and a big type of endothelin-2 proteins are described. The proteins contain the amino acid sequence: Cys Ser Cys Ser Ser Trp Leu Asp Lys Glu Cys Val Tyr Phe Cys His Leu Asp Ile Ile Trp.

Abstract: Disclosed is a vasoconstrictor peptide called Endothelin having a molecular weight of 2,500.+-.300. Endothelin contains 21 amino acid residues, wherein four cysteines form two sets of S--S bond.In order to obtain the vasoconstrictor factors of the present invention, it is preferable to culture the endothelial cells collected from the vascular inner walls in a serum-free medium.The vasoconstrictor peptides of the present invention can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.

Abstract: A compound is disclosed which has the formula, ##STR1## where R.sup.1 is a hydrogen atom, or a straight or branched chain alkyl group having 1 to 4 carbon atoms, and R.sup.2 is a straight or branched chain alkyl group having 1 to 4 carbon atoms, ##STR2## in which n is an integer of 0 to 3, or a pharmaceutically acceptable salt thereof. A process for producing the compound and a medicine containing the compound are also disclosed. Such medicine is effectively useful for inhibiting myocardial infarction.

Abstract: A compound is disclosed which has the formula, ##STR1## where R.sup.1 is a hydrogen atom, or a straight or branched chain alkyl group having 1 to 4 carbon atoms, and R.sup.2 is a straight or branched chain alkyl group having 1 to 4 carbon atoms, ##STR2## in which n is an integer of 0 to 3, or a pharmaceutically acceptable salt thereof. A process for producing the compound and a medicine containing the compound are also disclosed. Such medicine is effectively useful for inhibiting myocardial infarction.