Abstract: A method to efficiently extract and purify a target foreign protein expressed with the use of an E. coli strain from the periplasm is disclosed. The method for producing a protein comprising the steps (a) to (c) below: (a) a step of introducing a gene encoding a target foreign protein into E. coli having modification in a gene associated with maintenance of the outer membrane structure; (b) a step of culturing the E. coli of (a); and (c) a step of recovering a target foreign protein from the periplasmic fraction of the microbial cell after culture.

Abstract: A signal generation device includes a first timer to output an interrupt signal at a predetermined cycle, a second timer to output a first pulse signal and a second pulse signal, a third timer to count a number of edges of the first pulse signal and the second pulse signal, and a processor configured or programmed to perform interrupt processing to control an output mode of the second timer every time the interrupt signal occurs. When the interrupt frequency is equal to an initial value at the start of the interrupt processing, the processor is configured or programmed to perform predetermined short-period processing after performing predetermined long-period processing. When the interrupt frequency is not equal to the initial value, the processor is configured or programmed to perform the short-period processing without performing the long-period processing. The processor execute is configured or programmed to angle acquisition processing and function calculation processing as the long-period processing.

Abstract: A rotation angle detector is smaller and less expensive and has higher detection accuracy. A rotation angle detector for detecting a rotation angle of a rotator including at least two pole pairs arranged in a ring includes an electrical angle calculator that calculates an electrical angle of the rotator, a pole pair number setter that sets a pole pair number for each of the at least two pole pairs in the rotator, an origin pole pair detector that detects, based on magnitudes of magnetic fluxes of the at least two pole pairs, an origin pole pair as an origin and sets an origin pole pair number to the origin pole pair, and an absolute mechanical-angle calculator that calculates an absolute mechanical angle of the rotator based on the electrical angle, the pole pair number, and the origin pole pair number.

Abstract: A position estimation device acquires detection values of magnetic field strength at three or more locations of a rotor in a range where a rotor angle is less than one rotation. A section is selected based on a detection value of the magnetic field strength from predetermined sections for a pole pair number of the rotor. A feature amount calculator is provided to calculate feature amounts of a waveform of the magnetic field strength based on a combination of the detection values of the magnetic field strength according to the section selected. An estimator is provided to determine, for each segment associated with the section selected, whether or not a magnitude relationship of the feature amounts learned in advance coincides with a magnitude relationship of the feature amounts calculated, and estimating, as a rotation position of the rotor, the pole pair number associated with the segment having the same magnitude relationship.

Abstract: A signal generation device includes a first timer to output an interrupt signal at a predetermined cycle, a second timer to output a first pulse signal and a second pulse signal, a third timer to count a number of edges of the first pulse signal and the second pulse signal, and a processor configured or programmed to perform interrupt processing to control an output mode of the second timer every time the interrupt signal occurs. When the interrupt frequency is equal to an initial value at the start of the interrupt processing, the processor is configured or programmed to perform predetermined short-period processing after performing predetermined long-period processing. When the interrupt frequency is not equal to the initial value, the processor is configured or programmed to perform the short-period processing without performing the long-period processing. The processor execute is configured or programmed to angle acquisition processing and function calculation processing as the long-period processing.

Abstract: A position estimation device acquires detection values of magnetic field strength at three or more locations of a rotor in a range where a rotor angle is less than one rotation. A section is selected based on a detection value of the magnetic field strength from predetermined sections for a pole pair number of the rotor. A feature amount calculator is provided to calculate feature amounts of a waveform of the magnetic field strength based on a combination of the detection values of the magnetic field strength according to the section selected. An estimator is provided to determine, for each segment associated with the section selected, whether or not a magnitude relationship of the feature amounts learned in advance coincides with a magnitude relationship of the feature amounts calculated, and estimating, as a rotation position of the rotor, the pole pair number associated with the segment having the same magnitude relationship.

Abstract: A position estimation device acquires detection values of a magnetic field strength at three or more locations of a rotor in accordance with a rotor angle in a range where the rotor angle is less than one rotation. A feature amount calculator is provided to calculate feature amounts of a waveform of the magnetic field strength based on the detection values of the magnetic field strength. An estimator is provided to determine, for each pole pair number of the rotor, whether or not a pattern of a magnitude relationship of the feature amounts learned in advance coincides with a pattern of a magnitude relationship of the feature amounts calculated, and to estimate, as a rotation position of the rotor, the pole pair number with which a pattern of the magnitude relationship coincides.