Patents by Inventor Tomokazu Shirai
Tomokazu Shirai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230068606Abstract: It has been found that wild-type ferulic acid decarboxylase derived from Saccharomyces has high catalytic activity for the production of unsaturated hydrocarbon compounds. Further, it has been found that in the ferulic acid decarboxylase, substitute of the amino acid at position 398 with glutamine, methionine, asparagine, phenylalanine, histidine, or threonine more improves the catalytic activity, making it possible to provide a method capable of producing an unsaturated hydrocarbon compound such as butadiene with high productivity, and an enzyme used in the method.Type: ApplicationFiled: September 18, 2020Publication date: March 2, 2023Applicants: RIKEN, ZEON CORPORATION, THE Yokohama Rubber Co., Ltd.Inventors: Tomokazu SHIRAI, Yutaro MORI, Kazuhiro TAKAHASHI, Yayoi AKAHORI
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Publication number: 20220335092Abstract: Provided is a literature information service method using a single computer or a plurality of computers connected to each other via a network. The literature information service method includes: transmitting a first character string to a plurality of first servers connected respectively to a plurality of databases each including information of enzymes, and receiving a plurality of pieces of data obtained by searching the plurality of databases with the first character string; extracting a plurality of second character strings indicating information of enzymes from the plurality of pieces of data; generating a search expression using at least one of the plurality of extracted second character strings; and searching a literature database using the search expression to acquire search result data.Type: ApplicationFiled: June 4, 2020Publication date: October 20, 2022Inventors: Yohei YAMADA, Hiroko KAWASAKI, Akira HOSOYAMA, Seiha MIYAZAWA, Tomokazu SHIRAI
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Patent number: 11142778Abstract: With the aim of providing a method allowing production of an unsaturated hydrocarbon compound such as butadiene with high productivity and an enzyme used in the method, the present inventors introduced mutations involving amino acid replacement into various positions of a ferulic acid decarboxylase, and prepared multiple modified forms of the enzyme. Then, the present inventors evaluated those modified forms in terms of the catalytic activity for the production of butadiene, and found as a result that the catalytic activity was improved in the case where, for example, the amino acid at position 395 was glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, or phenylalanine.Type: GrantFiled: July 24, 2018Date of Patent: October 12, 2021Assignee: RIKENInventors: Tomokazu Shirai, Yutaro Mori
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Publication number: 20210130854Abstract: With the aim of providing a method allowing production of an unsaturated hydrocarbon compound such as butadiene with high productivity and an enzyme used in the method, the present inventors introduced mutations involving amino acid replacement into various positions of a ferulic acid decarboxylase, and prepared multiple modified forms of the enzyme. Then, the present inventors evaluated those modified forms in terms of the catalytic activity for the production of butadiene, and found as a result that the catalytic activity was improved in the case where, for example, the amino acid at position 395 was glutamine, histidine, asparagine, leucine, isoleucine, methionine, lysine, serine, arginine, tyrosine, or phenylalanine.Type: ApplicationFiled: July 24, 2018Publication date: May 6, 2021Applicant: RIKENInventors: Tomokazu SHIRAI, Yutaro MORI
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Patent number: 10988751Abstract: A method enables olefin compound production with a high productivity, and an enzyme is used in the method. Mutations involving amino acid substitution are introduced into various sites of diphosphomevalonate decarboxylase (MVD) to prepare multiple MVD variants. Then, these variants are evaluated in terms of catalytic activity for producing an olefin compound such as isoprene, and have found as a result that the catalytic activity is improved when serine at position 153 and threonine at position 209 are each substituted with a different amino acid. In addition, the catalytic activity of the variants is further improved when glycine at position 152 is further substituted with a different amino acid.Type: GrantFiled: January 30, 2018Date of Patent: April 27, 2021Assignees: RIKEN, ZEON CORPORATION, THE YOKOHAMA RUBBER CO., LTD.Inventors: Ryoko Orishimo, Tomokazu Shirai, Kazuhiro Takahashi, Misao Hiza, Yusuke Tanabe
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Diphosphomevalonate decarboxylase variant and method for producing olefin compound by using the same
Patent number: 10961548Abstract: Mutations involving amino acid substitution were introduced into various sites of diphosphomevalonate decarboxylase (MVD), thus preparing a large number of MVD variants. Then, the variants were each evaluated in terms of a catalytic activity for production of olefin compounds such as isoprene. As a result, it was found that substitution of glycine at position with a different amino acid resulted in improvement in the catalytic activity. In addition, it was found that the MVD in which arginine at position and threonine at position in addition to the position were further substituted with different amino acids, respectively, also had the high catalytic activity.Type: GrantFiled: September 26, 2017Date of Patent: March 30, 2021Assignees: RIKEN, ZEON CORPORATION, THE YOKOHAMA RUBBER CO., LTD.Inventors: Ryoko Orishimo, Tomokazu Shirai, Kazuhiro Takahashi, Misao Hiza, Yusuke Tanabe -
Patent number: 10781460Abstract: A method which enables olefin compound production with a high productivity and an enzyme used in the method, a mutation involving amino acid substitution has been introduced into various sites of diphosphomevalonate decarboxylase (MVD), thus preparing a large number of MVD variants. Next, the result of evaluating the variants for the catalytic activity related to the production of olefin compounds such as isoprene has revealed that MVD whose threonine at position 209 is substituted with a different amino acid has the catalytic activity, and that MVD whose arginine at position 74 is further substituted with a different amino acid in addition to position 209 has the catalytic activity at higher levels.Type: GrantFiled: August 3, 2016Date of Patent: September 22, 2020Assignees: RIKEN, ZEON CORPORATION, THE YOKOHAMA RUBBER CO., LTD.Inventors: Ryoko Orishimo, Tomokazu Shirai, Kazuhiro Takahashi, Misao Hiza, Yusuke Tanabe
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Publication number: 20190345476Abstract: A method enables olefin compound production with a high productivity, and an enzyme is used in the method. Mutations involving amino acid substitution are introduced into various sites of diphosphomevalonate decarboxylase (MVD) to prepare multiple MVD variants. Then, these variants are evaluated in terms of catalytic activity for producing an olefin compound such as isoprene, and have found as a result that the catalytic activity is improved when serine at position 153 and threonine at position 209 are each substituted with a different amino acid. In addition, the catalytic activity of the variants is further improved when glycine at position 152 is further substituted with a different amino acid.Type: ApplicationFiled: January 30, 2018Publication date: November 14, 2019Applicants: RIKEN, ZEON CORPORATION, The Yokohama Rubber Co., Ltd.Inventors: Ryoko ORISHIMO, Tomokazu SHIRAI, Kazuhiro TAKAHASHI, Misao HIZA, Yusuke TANABE
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DIPHOSPHOMEVALONATE DECARBOXYLASE VARIANT AND METHOD FOR PRODUCING OLEFIN COMPOUND BY USING THE SAME
Publication number: 20190264237Abstract: Mutations involving amino acid substitution were introduced into various sites of diphosphomevalonate decarboxylase (MVD), thus preparing a large number of MVD variants. Then, the variants were each evaluated in terms of a catalytic activity for production of olefin compounds such as isoprene. As a result, it was found that substitution of glycine at position with a different amino acid resulted in improvement in the catalytic activity. In addition, it was found that the MVD in which arginine at position and threonine at position in addition to the position were further substituted with different amino acids, respectively, also had the high catalytic activity.Type: ApplicationFiled: September 26, 2017Publication date: August 29, 2019Applicants: RIKEN, ZEON CORPORATION, THE YOKOHAMA RUBBER CO., LTD.Inventors: Ryoko ORISHIMO, Tomokazu SHIRAI, Kazuhiro TAKAHASHI, Misao HIZA, Yusuke TANABE -
Publication number: 20180245104Abstract: A method which enables olefin compound production with a high productivity and an enzyme used in the method, a mutation involving amino acid substitution has been introduced into various sites of diphosphomevalonate decarboxylase (MVD), thus preparing a large number of MVD variants. Next, the result of evaluating the variants for the catalytic activity related to the production of olefin compounds such as isoprene has revealed that MVD whose threonine at position 209 is substituted with a different amino acid has the catalytic activity, and that MVD whose arginine at position 74 is further substituted with a different amino acid in addition to position 209 has the catalytic activity at higher levels.Type: ApplicationFiled: August 3, 2016Publication date: August 30, 2018Applicants: RIKEN, ZEON CORPORATION, The Yokohama Rubber Co., Ltd.Inventors: Ryoko ORISHIMO, Tomokazu SHIRAI, Kazuhiro TAKAHASHI, Misao HIZA, Yusuke TANABE
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Publication number: 20180237813Abstract: The present invention provides a method for producing an aromatic compound such as salicylic acid and a derivative thereof with high productivity using a microorganism. The present invention provides: a method for producing a microorganism having a sugar metabolic pathway modified, including suppressing the expression of a gene encoding a phosphotransferase system enzyme of the microorganism, suppressing the expression of a gene encoding pyruvate kinase of the microorganism, and introducing, into the microorganism, one or more genes encoding an enzyme that enables the microorganism to synthesize an aromatic compound from chorismic acid or isochorismic acid; a modified microorganism obtained by the method; and a method for producing an aromatic compound and a derivative thereof, including culturing the microorganism, and recovering an aromatic compound or the like from the culture.Type: ApplicationFiled: August 24, 2016Publication date: August 23, 2018Applicant: RIKENInventors: Shuhei Noda, Tomokazu SHIRAI
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Patent number: 9822387Abstract: An acetyl-CoA-producing microorganism, which is capable of efficiently synthesizing acetyl-CoA using carbon dioxide, and a substance production method using the same are provided. An acetyl-CoA-producing microorganism including an acetyl-CoA production cycle obtained by imparting at least one type of enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism.Type: GrantFiled: July 27, 2012Date of Patent: November 21, 2017Assignee: MITSUI CHEMICALS, INC.Inventors: Ryota Fujii, Tomokazu Shirai, Tadashi Araki, Koh Amano, Yoshiko Matsumoto, Toshihiro Tateno, Nozomi Takebayashi, Takashi Morishige, Hitoshi Takahashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa, Tomonori Hidesaki, Ayako Endo, Dominik Lukas Jürgen-Lohmann, Anjali Madhavan, Su Sun Chong
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Patent number: 9267156Abstract: An isopropyl alcohol-producing Escherichia coli includes an isopropyl alcohol production system, wherein an activity of transcriptional repressor GntR is inactivated, and the isopropyl alcohol-producing Escherichia coli preferably further includes a group of auxiliary enzymes having an enzyme activity expression pattern with which isopropyl alcohol production capacity achieved by the inactivation of the GntR activity is maintained or enhanced. A method of producing isopropyl alcohol includes producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing Escherichia coli. A method of producing acetone includes contacting the isopropyl alcohol obtained by the isopropyl alcohol production method with a complex oxide that includes zinc oxide and at least one oxide containing a Group 4 element, and that is prepared by coprecipitation.Type: GrantFiled: August 11, 2011Date of Patent: February 23, 2016Assignee: MITSUI CHEMICALS, INC.Inventors: Koh Amano, Tomokazu Shirai, Hitoshi Takahashi, Junichiro Hirano, Yoshiko Matsumoto, Nozomi Takebayashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa
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Patent number: 8932845Abstract: An isopropyl alcohol-producing Escherichia coli equipped with an isopropyl alcohol production system, having at least one enhanced enzyme activity selected from the group consisting of an enhanced malate dehydrogenase activity, an enhanced NAD(P)+ transhydrogenase (AB-specific) activity, and an enhanced thiolase activity, and an isopropyl alcohol producing method including producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing Escherichia coli.Type: GrantFiled: March 4, 2011Date of Patent: January 13, 2015Assignee: Mitsui Chemicals, Inc.Inventors: Yoshiko Matsumoto, Junichiro Hirano, Takashi Morishige, Tomokazu Shirai, Hitoshi Takahashi, Koh Amano, Nozomi Takebayashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa
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Publication number: 20140363847Abstract: An acetyl-CoA-producing microorganism, which is capable of efficiently synthesizing acetyl-CoA using carbon dioxide, and a substance production method using the same are provided. An acetyl-CoA-producing microorganism including an acetyl-CoA production cycle obtained by imparting at least one type of enzymatic activity selected from the group consisting of malate thiokinase, malyl-CoA lyase, glyoxylate carboligase, 2-hydroxy-3-oxopropionate reductase, and hydroxypyruvate reductase, to a microorganism.Type: ApplicationFiled: July 27, 2012Publication date: December 11, 2014Inventors: Ryota Fujii, Tomokazu Shirai, Tadashi Araki, Koh Amano, Yoshiko Matsumoto, Toshihiro Tateno, Nozomi Takebayashi, Takashi Morishige, Hitoshi Takahashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa, Tomonori Hidesaki, Ayako Endo, Dominik Lukas Jürgen-Lohmann, Anjali Madhavan
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Publication number: 20130211170Abstract: An isopropyl alcohol-producing Escherichia coli includes an isopropyl alcohol production system, wherein an activity of transcriptional repressor GntR is inactivated, and the isopropyl alcohol-producing Escherichia coli preferably further includes a group of auxiliary enzymes having an enzyme activity expression pattern with which isopropyl alcohol production capacity achieved by the inactivation of the GntR activity is maintained or enhanced. A method of producing isopropyl alcohol includes producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing Escherichia coli. A method of producing acetone includes contacting the isopropyl alcohol obtained by the isopropyl alcohol production method with a complex oxide that includes zinc oxide and at least one oxide containing a Group 4 element, and that is prepared by coprecipitation.Type: ApplicationFiled: August 11, 2011Publication date: August 15, 2013Inventors: Koh Amano, Tomokazu Shirai, Hitoshi Takahashi, Junichiro Hirano, Yoshiko Matsumoto, Nozomi Takebayashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa
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Publication number: 20130005008Abstract: An isopropyl alcohol-producing Escherichia coli equipped with an isopropyl alcohol production system, having at least one enhanced enzyme activity selected from the group consisting of an enhanced malate dehydrogenase activity, an enhanced NAD(P)+ transhydrogenase (AB-specific) activity, and an enhanced thiolase activity, and an isopropyl alcohol producing method including producing isopropyl alcohol from a plant-derived raw material using the isopropyl alcohol-producing Escherichia coli.Type: ApplicationFiled: March 4, 2011Publication date: January 3, 2013Inventors: Yoshiko Matsumoto, Junichiro Hirano, Takashi Morishige, Tomokazu Shirai, Hitoshi Takahashi, Koh Amano, Nozomi Takebayashi, Mitsufumi Wada, Hiroshi Shimizu, Chikara Furusawa, Takashi Hirasawa