Abstract: The zinc-base plated steel sheet has a steel sheet, a zinc-base plating layer on the steel sheet, and a composite coating film formed on the plating layer, which composite coating layer contains a P ingredient, an N ingredient, and at least one element selected from the group consisting of Mg, Al, Ca, Ti, Fe, Co, Ni, Cu, and Mo, and is prepared by applying an aqueous solution containing a cationic ingredient (?) and a phosphoric acid ingredient (?) onto the surface of plating layer on the zinc-base plated steel sheet, and then drying the applied aqueous solution, without giving washing with water, and which cationic ingredient (?) consists essentially of at least one metallic ion selected from the group consisting of Mg, Al, Ca, Ti, Fe, Co, Ni, Cu, Mo, and NH4+ ions.

Abstract: The present invention provides a HLA-typing technique that uses samples that are safe and convenient for storage and transportation and therefore can significantly reduce the cost associated with the analysis and ensure high accuracy of the analysis as well as high processing efficiency. A method for HLA typing, comprising the steps of: directly amplifying DNA from a biological sample; determining a base sequence of DNA in the biological sample; and determining HLA type on the basis of the base sequence. Preferably, the DNA amplification reaction is Polymerase Chain Reaction, the biological sample is a paper-spotted blood sample obtained by spotting blood on filter paper and drying the blood.

Abstract: The zinc-base plated steel sheet has a steel sheet, a zinc-base plating layer on the steel sheet, and a composite coating film formed on the plating layer, which composite coating layer contains a P ingredient, an N ingredient, and at least one element selected from the group consisting of Mg, Al, Ca, Ti, Fe, Co, Ni, Cu, and Mo, and is prepared, by applying an aqueous solution containing a cationic ingredient (&agr;) and a phosphoric acid ingredient (&bgr;) onto the surface of plating layer on the zinc-base plated steel sheet, and then drying the applied aqueous solution, without giving washing with water, and which cationic ingredient (&agr;) consists essentially of at least one metallic ion selected from the group consisting of Mg, Al, Ca, Ti, Fe, Co, Ni, Cu, Mo, and NH4+ ions.

Abstract: The invention provides a galvannealed steel sheet which has an oxide layer having 10 nm or larger thickness on the plateau of the coating layer flattened by temper rolling. With the use of the galvannealed steel sheet, no powdering occurs during press-forming, and stable and excellent sliding performance is attained. By selecting the area percentage of the plateau of the flattened coating layer to a range from 20 to 80%, making the coating layer single layer of &dgr;1 phase, and letting &zgr; phase exist in the &dgr;1 phase, further improved sliding performance and anti-powdering property are obtained.

Abstract: To provide UDP-D-glucose:limonoid glucosyltransferase, and DNA coding for the enzyme.

Abstract: A surface treating method is described, which method comprising applying, between electrodes, a potential sufficient to cause corona discharge to occur in the presence of a gas which comprises molecules containing at least one atom selected from the group consisting of halogen atom, oxygen atom and nitrogen atom. The resultant corona discharge is applied to an object to be treated for the surface treatment of the object, said object being outside said electrodes. The excellent adhesive surface can be obtained when said object is separated from said electrodes at a distance in the range of 10 mm to 5 m.