Abstract: The present invention addresses the problem of providing: a culture medium that is for adherent culturing of pluripotent stem cells exhibiting high growth ability and that does not contain serum or albumin; and a highly versatile method for adherent culturing of pluripotent stem cells using the culture medium. According to the present invention, by adding, to a culture medium, a hydrophilic polymer that is conventionally known to inhibit adhesion of cells to the surface of a cell culture base material, so that the adhesion of pluripotent stem cell to the surface of the cell culture base material is enhanced, high growth ability is imparted in adherent culturing of pluripotent stem cells without adding serum or albumin thereto. In addition, by adding an antioxidant substance along with such a polymer to a culture medium, very high growth ability is imparted in adherent culturing of pluripotent stem cells.

Abstract: A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.

Abstract: A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.

Abstract: A single cell-culture chamber is provided that includes a dried vitrigel membrane covering and secured to one open end surface of a tubular frame. Also provided is a double cell-culture chamber that includes two tubular frames of substantially the same planar cross-sectional shape adhesively secured to each other with a dried vitrigel membrane interposed between the opposing open end surfaces of the tubular frames so as to form a first chamber and a second chamber via the dried vitrigel membrane.

Abstract: A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.