Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.

Abstract: Provided is a method capable of producing a protein at a high level using a cultured animal cell, comprising culturing a cell that expresses APES (Antibody Production Enhancing Sequence) and into which a DNA encoding a desired polypeptide has been introduced, thereby producing the desired polypeptide. APES contains a nucleotide sequence related to NfkBia and has a function of decreasing the intracellular expression of NfkBia.

Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.

Abstract: The present invention provides a cell capable of high-yield production of polypeptides and a method for producing the same. The present invention relates to a method for producing a cell capable of high-yield production of a desired polypeptide, wherein a strongly bicarbonate transporter-expressing cell into which DNA encoding the desired polypeptide has been introduced is cultured in the presence of a high concentration of methotrexate and a cell capable of high-yield production of the desired polypeptide is selected from among surviving cells.

Abstract: A base frame 1 is such as to be fixedly provided on a body so that a parking brake device 2 is provided thereon which causes a brake applying portion of a parking brake to apply the brake and release the brake so applied, a base frame main body 1a is molded into an integral unit, a parking device mounting portion 10A where the parking brake device 2 is mounted is formed on one side A of the base frame man body 1a, and a gearshift device mounting portion 10B where a gearshift device of a transmission is provided is formed on the other side B thereof.

Abstract: The present invention provides a method capable of producing a protein efficiently. A method of producing a polypeptide, comprising culturing a cell which strongly expresses a bicarbonate transporter and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce said polypeptide.

Abstract: The present invention provides a cell capable of high-yield production of polypeptides and a method for producing the same. The present invention relates to a method for producing a cell capable of high-yield production of a desired polypeptide, wherein a strongly taurine transporter-expressing cell into which DNA encoding the desired polypeptide has been introduced is cultured in the presence of a high concentration of methotrexate and a cell capable of high-yield production of the desired polypeptide is selected from among surviving cells.

Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses cysteine sulfinic acid decarboxylase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster cysteine sulfinic acid decarboxylase, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.

Abstract: The present invention provides a method capable of producing a natural or recombinant protein in high yield. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses alanine aminotransferase and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide.

Abstract: The present invention provides a method capable of producing a natural or recombinant protein at low cost. The present invention relates to a method of producing a polypeptide, comprising culturing a cell which strongly expresses a taurine transporter and has a transferred DNA encoding a desired polypeptide and thereby allowing the cell to produce the polypeptide. Hamster taurine transporter, a DNA encoding the same, a recombinant vector and a transformed cell are also provided.

Abstract: A parking brake apparatus has a base, a ratchet plate fixed to the base, an operation lever includes a rotational base portion pivotally supported on the base; a pawl adapted to engage/disengage with the ratchet plate; an arc-shaped intermediate member comprises an arc-shaped relay rod; and a grip for controlling the engagement of the pawl. The arc-shaped relay rod is divided into at least two and is made to be interlocked with operations by the grip to transmit an engagement/disengagement operation with the ratchet plate to the pawl, the ratchet plate and the rotational base portion are provided inside a body side mounting portion, and the intermediate member is provided in such a manner as to appear from and disappear into a passage hole in the body side mounting portion.

Abstract: A parking brake apparatus has a base, a ratchet plate fixed to the base, an operation lever includes a rotational base portion pivotally supported on the base; a pawl adapted to engage/disengage with the ratchet plate; an arc-shaped intermediate member comprises an arc-shaped relay rod; and a grip for controlling the engagement of the pawl. The arc-shaped relay rod is divided into at least two and is made to be interlocked with operations by the grip to transmit an engagement/disengagement operation with the ratchet plate to the pawl, the ratchet plate and the rotational base portion are provided inside a body side mounting portion, and the intermediate member is provided in such a manner as to appear from and disappear into a passage hole in the body side mounting portion.

Abstract: A base frame 1 is such as to be fixedly provided on a body so that a parking brake device 2 is provided thereon which causes a brake applying portion of a parking brake to apply the brake and release the brake so applied, a base frame main body la is molded into an integral unit, a parking device mounting portion 10A where the parking brake device 2 is mounted is formed on one side A of the base frame man body 1a, and a gearshift device mounting portion 10B where a gearshift device of a transmission is provided is formed on the other side B thereof.

Abstract: A parking brake device which can not only better the safety, but also reduce the cost by simplifying the construction. A shock absorbing portion 35, 36 which resists an operating force in pulling a brake lever 30 toward the compartment and which is deformed by an impact force in the direction which is approximately opposite to the direction of pulling the brake lever 30 toward the compartment, absorbing the impact force is provided.

Abstract: A parking brake device which can not only better the safety, but also reduce the cost by simplifying the construction. A shock absorbing portion 35, 36 which resists an operating force in pulling a brake lever 30 toward the compartment and which is deformed by an impact force in the direction which is approximately opposite to the direction of pulling the brake lever 30 toward the compartment, absorbing the impact force is provided.