Abstract: The present invention provides a method for treating a solution contaminated with porcine circovirus, comprising the steps of: adjusting a pH of a solution contaminated with porcine circovirus to range from 3.0 to 7.0; and treating the pH-adjusted solution with a filtration membrane having an LRV for bacteriophage PP7 of 4 or more.

Abstract: The object of the present invention is to provide a differentiation inhibiting agent which allows culture of a stem cell or an embryonic stem cell in an undifferentiated state without use of any feeder cell, a method for culturing using the same, a cell culture liquid using the same, and a cell prepared by culturing using this differentiation inhibiting agent. The present invention provides a differentiation inhibiting agent which comprises a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient; a method for safely culturing a stem cell in large scale in undifferentiated state in the absence of feeder cell which comprises culturing a stem cell by using a tetrahydroisoquinoline derivative; a culture liquid for stem cells comprising a tetrahydroisoquinoline derivative; and a cell which is obtained by culture using a tetrahydroisoquinoline derivative as a differentiation inhibiting agent.

Abstract: A system and method for protein separation and purification includes a mixing loop, a primary pump having an inlet in communication with a supply of an acid or an alkali and an outlet in communication with the mixing loop. A concentrate pump has an inlet in communication with a supply of an acid or an alkali and an outlet in communication with the mixing loop. A liquid chromatography column is in communication with the mixing loop and is packed with an ion exchange resin and loaded with a protein solution containing a first protein having a first isoelectric point and a second protein having a second isoelectric point. A process control feedback loop in communication with the mixing loop includes an inline process pH analyzer so that a pH of a titration solution in the mixing loop may be detected and adjusted based on the detected pH.

Abstract: The object of the present invention is to provide a differentiation inhibiting agent which allows culture of a stem cell or an embryonic stem cell in an undifferentiated state without use of any feeder cell, a method for culturing using the same, a cell culture liquid using the same, and a cell prepared by culturing using this differentiation inhibiting agent. The present invention provides a differentiation inhibiting agent which comprises a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient; a method for safely culturing a stem cell in large scale in undifferentiated state in the absence of feeder cell which comprises culturing a stem cell by using a tetrahydroisoquinoline derivative; a culture liquid for stem cells comprising a tetrahydroisoquinoline derivative; and a cell which is obtained by culture using a tetrahydroisoquinoline derivative as a differentiation inhibiting agent.

Abstract: According to the present invention, capable of safely holding a large amount of undifferentiated embryonic stem cells to culture in the absence of feeder cells or feeder cell-derived components. Cultured embryonic stem cells can be applied to the fields of cell culture, tissue transplantation, drug development, and gene therapy.

Abstract: The object of the present invention is to provide a differentiation inhibiting agent which allows culture of a stem cell or an embryonic stem cell in an undifferentiated state without use of any feeder cell, a method for culturing using the same, a cell culture liquid using the same, and a cell prepared by culturing using this differentiation inhibiting agent. The present invention provides a differentiation inhibiting agent which comprises a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient; a method for safely culturing a stem cell in large scale in undifferentiated state in the absence of feeder cell which comprises culturing a stem cell by using a tetrahydroisoquinoline derivative; a culture liquid for stem cells comprising a tetrahydroisoquinoline derivative; and a cell which is obtained by culture using a tetrahydroisoquinoline derivative as a differentiation inhibiting agent.