Abstract: The present invention provides efficient methods based on alteration of the protein A-binding ability, for producing or purifying multispecific antibodies having the activity of binding to two or more types of antigens to high purity through a protein A-based purification step alone. The methods of the present invention for producing or purifying multispecific antibodies which feature altering amino acid residues of antibody heavy chain constant region and/or variable region. Multispecific antibodies with an altered protein A-binding ability, which exhibit plasma retention comparable or longer than that of human IgG1, can be efficiently prepared in high purity by introducing amino acid alterations of the present invention into antibodies.

Abstract: A two-wheeled vehicle tire achieves a further improvement particularly in the traction performance during turning on soft ground. A two-wheeled vehicle tire includes a tread portion formed in an annular shape. The tread portion includes: center land portions arranged on a tire equator CL; middle land portions arranged on the tire width-direction outer side of the center land portions; and shoulder land portions arranged on the tire width-direction outer side of the middle land portions, and T-shaped or Y-shaped land portions each constituted by three partial land portions connected at one end are arranged between the center land portions and the shoulder land portions.

Abstract: A two-wheeled vehicle tire achieves a further improvement particularly in the traction performance during turning on soft ground. A two-wheeled vehicle tire includes a tread portion formed in an annular shape. The tread portion includes: center land portions arranged on a tire equator CL; middle land portions arranged on the tire width-direction outer side of the center land portions; and shoulder land portions arranged on the tire width-direction outer side of the middle land portions, and T-shaped or Y-shaped land portions each constituted by three partial land portions connected at one end are arranged between the center land portions and the shoulder land portions.