Patents by Inventor Tonino Alonzi

Tonino Alonzi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Conditioned cell culture medium, method to obtain the same and use of it for maintenance, proliferation and differentiation of mammalian cells
    Patent number: 7723105
    Abstract: The invention relates to a conditioned cell culture medium and a corresponding method to obtain it. The invention also refers to methods of using this cellconditioned medium for the maintenance, proliferation and differentiation of mammalian cells. The culture medium produced in accordance with the present invention is conditioned by the cell secretion activity of murine cells, in particular, those differentiated and immortalized transgenic hepatocytes, named MMH (Met Murine Hepatocyte). These media are employed in in vitro cell culture systems to induce maintenance, proliferation and differentiation of mammalian cells. The cells named MMH are differentiated non transformed murine hepatocytes that produce important biological molecules (e.g cytokines and growth factors) and, in accordance with the present invention, they are used in in vitro cell culture systems for the maintenance, proliferation and differentiation of mammalian cells.
    Type: Grant
    Filed: August 10, 2004
    Date of Patent: May 25, 2010
    Assignee: Instituto Nazionale per le Malattie
    Inventors: Veronica Bordoni, Tonino Alonzi, Marco Tripodi
  • Conditioned cell culture medium, method to obtain the same and use of it for maintenance, proliferation and differentiation of mammalian cells
    Publication number: 20060286081
    Abstract: The invention relates to a conditioned cell culture medium and a corresponding method to obtain it. The invention also refers to methods of using this cellconditioned medium for the maintenance, proliferation and differentiation of mammalian cells. The culture medium produced in accordance with the present invention is conditioned by the cell secretion activity of murine cells, in particular, those differentiated and immortalized transgenic hepatocytes, named MMH (Met Murine Hepatocyte). These media are employed in in vitro cell culture systems to induce maintenance, proliferation and differentiation of mammalian cells. The cells named MMH are differentiated non transformed murine hepatocytes that produce important biological molecules (e.g cytokines and growth factors) and, in accordance with the present invention, they are used in in vitro cell culture systems for the maintenance, proliferation and differentiation of mammalian cells.
    Type: Application
    Filed: August 10, 2004
    Publication date: December 21, 2006
    Inventors: Veronica Bordoni, Tonino Alonzi