Abstract: An apparatus and method for separating a biomolecule from one or more contaminants in a sample. The apparatus can include a unitary device comprising a plurality of fractionation devices, each reservoir comprising a filter, each reservoir adapted to contain a solid phase, the solid phase adapted to separate the biomolecule and the contaminant by fractionation. The filter can have an average pore size that allows the sample to pass therethrough while substantially preventing the solid phase from passing therethrough. The method can include moving the sample past the solid phase in each reservoir to separate the biomolecule from the contaminant by fractionation to obtain an isolated biomolecule.

Abstract: An apparatus and method for separating a biomolecule from one or more contaminants in a sample. The apparatus can include a unitary device comprising a plurality of fractionation devices, each reservoir comprising a filter, each reservoir adapted to contain a solid phase, the solid phase adapted to separate the biomolecule and the contaminant by fractionation. The filter can have an average pore size that allows the sample to pass therethrough while substantially preventing the solid phase from passing therethrough. The method can include moving the sample past the solid phase in each reservoir to separate the biomolecule from the contaminant by fractionation to obtain an isolated biomolecule.

Abstract: The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.

Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Abstract: Disclosed are methods of separating a target molecule from a non-target molecule using zinc- or cobalt-charged solid supports.

Abstract: Disclosed are methods of separating a target molecule from a non-target molecule using zinc- or cobalt-charged solid supports.

Abstract: Disclosed are methods of separating a target molecule from a non-target molecule using zinc- or cobalt-charged solid supports.

Abstract: The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.

Abstract: The present invention provides compositions for the separation of metals or biomolecules such as polypeptides, nucleic acids, or endotoxins using modified solid supports.