Abstract: Herein is reported a method for determining antibody-FcRn-interaction comprising the steps of immobilizing FcRn on a solid surface, which is suitable for surface plasmon resonance measurement, individually applying to the solid surface obtained in step a) solutions comprising the antibody at different concentrations and determining the association rate constant and the dissociation rate constant for each concentration, and determining with the rates obtained in step b) the KD-value of the antibody-FcRn-interaction, wherein the immobilized FcRn is monomeric FcRn, the monomeric FcRn is immobilized using functional (capture) groups that are directly attached to said solid surface, the solid surface is free of branched glucan, and the immobilization is at a pH value of from pH 7 to pH 8.

Abstract: The present invention generally relates to antibodies that bind to GPRC5D, including bispecific antigen binding molecules e.g. for activating T cells. In addition, the present invention relates to polynucleotides encoding such antibodies, and vectors and host cells comprising such polynucleotides. The invention further relates to methods for producing the antibodies, and to methods of using them in the treatment of disease.