Abstract: Quantification in a straightforward manner in an mRNA expression analysis is accomplished by using biotinylated oligo d(T) and coupling to a solid support (e.g., streptavidin-derivatized magnetic beads). Labeled targets specific to certain mRNAs of interest that are to be identified and quantified are added to a biological sample containing the mRNAs and biotinylated oligo d(T). The beads are preferably added after hybridization, but may be earlier added. Following attachment to the beads, unbound and non-specifically bound targets and non-mRNA material are removed by successive stringent washings. All the mRNA material is then eluted from the beads by subjection to conditions that separate the poly A and the oligo d(T), and the original mRNAs are then degraded by basic hydrolysis to leave single-strand synthetic targets, which are then hybridized to their specific probes suitably carried on a microarray.