Abstract: The present invention provides a dehydrogenase mutant L283V/L286V, and a preparation method and use thereof, and relates to the field of biomedicine technologies. An amino acid sequence of the mutant L283V/L286V is as shown in SEQ ID NO: 1; and the mutant is prepared by simultaneously mutating 283rd and 286th leucine of a dehydrogenase with an amino acid sequence as shown in SEQ ID NO: 3 into valine. The dehydrogenase mutant L283V/L286V shows high selectivity in catalyzing myosmine reduction reaction in a whole cell system to produce S-nornicotine, and has relatively high dehydrogenase and imine reductase activities, a short enzyme reduction time, and a high transformation rate. The product S-nornicotine obtained through the reaction has extremely high optical purity, which reduces the operation difficulty of subsequent purification.

Abstract: A system is described for authenticating a user on a client device using the user's mobile device and utilizing the audio channel. An authentication server receives a request from the client to initiate a session for the user, creates the session, and sends a session token back to the client along with a request for authentication. The client broadcasts an audio transmission containing the token to the mobile device over an audio channel using data-over-sound transmission. The mobile device receives the transmission via a microphone, obtains the token and the server identity from the transmission, and sends user credentials that are stored on the mobile device along with the token identifying the session directly to the authentication server. The server verifies the received credentials, confirms the token, and logs the user into the session.

Abstract: The present invention provides a dehydrogenase mutant L283V/L286V, and a preparation method and use thereof, and relates to the field of biomedicine technologies. An amino acid sequence of the mutant L283V/L286V is as shown in SEQ ID NO: 1; and the mutant is prepared by simultaneously mutating 283rd and 286th leucine of a dehydrogenase with an amino acid sequence as shown in SEQ ID NO: 3 into valine. The dehydrogenase mutant L283V/L286V shows high selectivity in catalyzing myosmine reduction reaction in a whole cell system to produce S-nornicotine, and has relatively high dehydrogenase and imine reductase activities, a short enzyme reduction time, and a high transformation rate. The product S-nornicotine obtained through the reaction has extremely high optical purity, which reduces the operation difficulty of subsequent purification.

Abstract: The present teaching relates to method, system, medium, and implementations for managing a virtual studio. A plurality of single data streams from a plurality content contributors are received. When a request is received, via public network connections, for creating a composite data stream associated with a virtual room in the virtual studio, signaling information is generated for constructing the composite data stream by stitching together some of the plurality of single data streams selected to be incorporated in the composite data stream in accordance with a layout. When an access request is received from an end user to access the composite data stream, the composite data stream is delivered to the end user in response to the access request.

Abstract: Provided is a system and a method for estimating capacity. The system includes one or more sensors, a compressor coupled to one or more sensors, and a controller. The controller is configured to receive one or more parameters of a cooling system, receive system state information and one or more measurements from the cooling system (306, 308), and compute a cooling capacity (304) based at least in part on the one or more parameters, one or more measurements and system state information. The system is also configured to estimate cooling capacity based on one or more computed capacity over a period of time (310), and provide the estimated capacity of the cooling system to a device in real-time.

Abstract: Heat pump systems and methods of operating including a first unit having a first unit heat exchanger, a compressor, an accumulator, and a first unit expansion valve, a second unit fluidly connected to the first unit by piping, the second unit having a second unit heat exchanger, and a system controller, the system controller having (I) a PID control element receiving as inputs (i) gain scheduling, (ii) an error signal, and (iii) feedback relating to an opening command of the first unit expansion valve, and (II) a feedforward control element generating a feedforward term that is combined with an output of the PID control element to generate the opening command of the first unit expansion valve. The system controller controls an opening of the first unit expansion valve using the opening command of the first unit expansion valve.

Abstract: The present teaching relates to method, system, medium, and implementations for managing a virtual studio. A plurality of single data streams from a plurality content contributors are received. When a request is received, via public network connections, for creating a composite data stream associated with a virtual room in the virtual studio, signaling information is generated for constructing the composite data stream by stitching together some of the plurality of single data streams selected to be incorporated in the composite data stream in accordance with a layout. When an access request is received from an end user to access the composite data stream, the composite data stream is delivered to the end user in response to the access request.

Abstract: Heat pump systems and methods of operating including a first unit having a first unit heat exchanger, a compressor, an accumulator, and a first unit expansion valve, a second unit fluidly connected to the first unit by piping, the second unit having a second unit heat exchanger, and a system controller, the system controller having (I) a PID control element receiving as inputs (i) gain scheduling, (ii) an error signal, and (iii) feedback relating to an opening command of the first unit expansion valve, and (II) a feedforward control element generating a feedforward term that is combined with an output of the PID control element to generate the opening command of the first unit expansion valve. The system controller controls an opening of the first unit expansion valve using the opening command of the first unit expansion valve.

Abstract: A system is described for authenticating a user on a client device using the user's mobile device and utilizing the audio channel. An authentication server receives a request from the client to initiate a session for the user, creates the session, and sends a session token back to the client along with a request for authentication. The client broadcasts an audio transmission containing the token to the mobile device over an audio channel using data-over-sound transmission. The mobile device receives the transmission via a microphone, obtains the token and the server identity from the transmission, and sends user credentials that are stored on the mobile device along with the token identifying the session directly to the authentication server. The server verifies the received credentials, confirms the token, and logs the user into the session.

Abstract: A method and apparatus for locally etching a substrate area the method including providing a substrate comprising a process surface; depositing a material layer over the process surface; and, applying a wet etchant to cover a targeted etching portion of the process surface while excluding an adjacent surrounding area to selectively etch the material layer overlying the targeted etching portion.

Abstract: A method and apparatus for locally etching a substrate area the method including providing a substrate comprising a process surface; depositing a material layer over the process surface; and, applying a wet etchant to cover a targeted etching portion of the process surface while excluding an adjacent surrounding area to selectively etch the material layer overlying the targeted etching portion.

Abstract: The invention involves a system and a method of inspecting a selected part design from an inventory of computer-aided part designs for die compliance as to the part design's geometric characteristics of die lock, draft, and sharp edge. The invention provides for an improvement of inspecting a computer-aided part design in order to lessen insufficient inspections which lead to manufacturing infeasibilities. The invention includes selecting a part design, defining a die open direction, evaluating die lock and draft characteristics of the selected part design, and evaluating sharp edge characteristics of the selected part design.