Abstract: The present invention provides recombinant gram-negative host cells that do not degrade protease-sensitive recombinant proteins yet grow to high cell density, methods for the use of these host cells to produce high-quality recombinant proteins, including antibodies and antibody fragments, at high yield, as well as compositions and methods relating to periplasmic expression of recombinant proteins or polypeptides of interest in host cells.

Abstract: Provided herein are methods of production of recombinant Erwinia asparaginase. Methods herein produce asparaginase having high expression levels in the periplasm or the cytoplasm of the host cell having activity comparable to commercially available asparaginase preparations.

Abstract: Provided herein are methods of production of recombinant E. coli asparaginase. Methods herein allow production of asparaginase in Pseudomonadales host cells at high expression levels and having activity comparable to commercially available asparaginase preparations.

Abstract: Provided herein are methods of production of recombinant Erwinia asparaginase. Methods herein produce asparaginase having high expression levels in the periplasm or the cytoplasm of the host cell having activity comparable to commercially available asparaginase preparations.

Abstract: Provided herein are methods of production of recombinant Erwinia asparaginase. Methods herein produce asparaginase having high expression levels in the periplasm or the cytoplasm of the host cell having activity comparable to commercially available asparaginase preparations.

Abstract: Provided herein are methods of production of recombinant E. coli asparaginase. Methods herein allow production of asparaginase in Pseudomonadales host cells at high expression levels and having activity comparable to commercially available asparaginase preparations.

Abstract: Provided herein are methods of production of recombinant E. coli asparaginase. Methods herein allow production of asparaginase in Pseudomonadales host cells at high expression levels and having activity comparable to commercially available asparaginase preparations.

Abstract: Provided herein are methods of production of recombinant E. coli asparaginase. Methods herein allow production of asparaginase in Pseudomonadales host cells at high expression levels and having activity comparable to commercially available asparaginase preparations.

Abstract: Provided herein are methods of production of recombinant Erwinia asparaginase. Methods herein produce asparaginase having high expression levels in the periplasm or the cytoplasm of the host cell having activity comparable to commercially available asparaginase preparations.

Abstract: A method for monitoring fluid media such as a dynamic biological system in a biological reactor containing developing culture fluid media. The method includes the step of directing light into the fluid media by way of one or more optical fibers to produce an illuminated fluid media and then measuring the intensity of light reflected from the illuminated fluid media by way of one or more optical fibers, the optical fibers being partitioned from the fluid media by a transparent window having inner and outer surfaces; the fibers having ends terminating adjacent to and confronting the inner surface of the window and extending in a direction away from the window, the corresponding ends of the fibers being radially and circumferentially spaced from one another, the corresponding ends of the fibers having converging and intersecting longitudinal projections therefrom, the intersecting longitudinal projections from the fibers being entirely within the window. Additionally, a fiver optic probe is discussed.