Patents by Inventor Torben Vedel Borchert
Torben Vedel Borchert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20040048247Abstract: The present invention relates to a method for producing a variant of a parent pullulanase, the variant having at least one altered property as compared to the parent pullulanase. The invention also relates to pullulanase variants and to the use of pullulanase variants of the invention for use in particular starch conversion processes.Type: ApplicationFiled: February 19, 2002Publication date: March 11, 2004Inventors: Allan Svendsen, Carsten Andersen, Torben Vedel Borchert
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Publication number: 20040048351Abstract: The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: ApplicationFiled: August 20, 2003Publication date: March 11, 2004Applicant: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen
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Publication number: 20040038368Abstract: The invention relates to a variant of a parent Termamyl-like &agr;-amylase, which exhibits an alteration in at least one of the following properties relative to said parent &agr;-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.Type: ApplicationFiled: September 19, 2003Publication date: February 26, 2004Applicant: Novozymes A/SInventors: Torben Vedel Borchert, Allan Svendsen, Carsten Andersen, Bjarne Nielsen, Torben Lauesgaard Nissen, Soren Kjaerulff
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Patent number: 6673589Abstract: The invention relates to a variant of a parent Termamyl-like &agr;-amylase, which exhibits an alteration in at least one of the following properties relative to said parent &agr;-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.Type: GrantFiled: January 25, 2001Date of Patent: January 6, 2004Assignee: Novozymes A/SInventors: Torben Vedel Borchert, Allan Svendsen, Carsten Andersen, Bjarne Nielsen, Torben Lauesgaard Nissen, Søren Kjærulff
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Publication number: 20030211958Abstract: The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).Type: ApplicationFiled: December 23, 2002Publication date: November 13, 2003Applicant: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard
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Patent number: 6642044Abstract: The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: GrantFiled: June 28, 2002Date of Patent: November 4, 2003Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisg{dot over (a)}rd-Frantzen
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Patent number: 6623948Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having alpha-amylase activity [E.C. 3.2.1.1], which may be derived from Bacillus. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.Type: GrantFiled: March 31, 2000Date of Patent: September 23, 2003Assignee: Novozymes A/SInventors: Helle Outtrup, Lisbeth Hedegaard Hoeck, Bjarne Ronfeldt Nielsen, Torben Vedel Borchert, Vibeke Skovgaard Nielsen, Henrik Bisgård-Frantzen, Allan Svendsen, Carsten Andersen
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Publication number: 20030170769Abstract: The present invention relates to a method of constructing a variant of a parent Termamyl-like &agr;-amylase, which variant has &agr;-amylase activity and at least one altered property as compared to the parent &agr;-amylase, comprisesType: ApplicationFiled: June 28, 2002Publication date: September 11, 2003Applicant: Novozymes A/SInventors: Allan Svendsen, Henrik Bisgard-Frantzen, Torben Vedel Borchert
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Publication number: 20030171236Abstract: The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: ApplicationFiled: June 28, 2002Publication date: September 11, 2003Applicant: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen
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Publication number: 20030129718Abstract: The present invention relates to variants (mutants) of polypeptides, in particular Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency, specific activity, and solubility, in particular under production conditions.Type: ApplicationFiled: August 9, 2001Publication date: July 10, 2003Applicant: Novozymes A/SInventors: Carsten Andersen, Torben Vedel Borchert, Bjarne Ronfeldt Nielsen
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Publication number: 20030064908Abstract: The present invention relates to variants of a parent &agr;-amylase, which parent &agr;-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No.Type: ApplicationFiled: December 19, 2001Publication date: April 3, 2003Applicant: Novozymes A/SInventors: Henrik Bisgard-Frantzen, Allan Svendsen, Torben Vedel Borchert
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Patent number: 6541207Abstract: A method for in vitro construction of a library of recombined homologous polynucleotides from a number of different starting DNA templates and primers by induced template shifts during an polynucleotide synthesis is described, whereby A. extended primers are synthesized by a) denaturing the DNA templates b) annealing primers to the templates, c) extending the said primers by use of a polymerase, d) stop the synthesis, and e) separate the extended primers from the templates, B. a template shift is induced by a) isolating the extended primers from the templates and repeating steps A.b) to A.e) using the extended primers as both primers and templates, or b) repeating steps A.b) to A.e), C. this process is terminated after an appropriate number of cycles of process steps A. and B.a), A. and B.b), or combinations thereof. Optionally the polynucleotides are amplified in a standard PCR reaction with specific primers to selectively amplify homologous polynucleotides of interest.Type: GrantFiled: October 13, 2000Date of Patent: April 1, 2003Assignee: Novozymes A/SInventors: Jesper Vind, Torben Vedel Borchert
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Patent number: 6528298Abstract: The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).Type: GrantFiled: April 7, 2000Date of Patent: March 4, 2003Assignee: Novozymes, A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard
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Patent number: 6518042Abstract: A process for making DNA libraries in filamentous fungal cells using a novel cloned gene involved in the mismatch repair system of filamentous fungal cells.Type: GrantFiled: February 24, 2000Date of Patent: February 11, 2003Assignee: Novozymes A/SInventors: Torben Vedel Borchert, Lars Christiansen, Jesper Vind
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Patent number: 6500644Abstract: A method for in vivo production of a library in cells comprising a multitude of mutated genetic elements, wherein an error-prone polymerase is used in each ancestral cell to replicate all or a part of a genetic element independently of the host chromosomal replication machinery. The genetic element comprises i) an origin of replication from which replication is initiated, ii) optionally a genetic marker, e.g. a gene conferring resistance towards an antibiotic, iii) a gene encoding the polypeptide of interest. Also methods for the generation of a DNA sequence encoding a desired variant of a polypeptide of interest, and for the determination of such a DNA sequence are described.Type: GrantFiled: September 26, 2000Date of Patent: December 31, 2002Assignee: Novozymes A/SInventors: Torben Vedel Borchert, Stanislas Dusko Ehrlich
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Patent number: 6440716Abstract: The present invention relates to a method of constructing a variant of a parent Termamyl-like &agr;-amylase, which variant has &agr;-amylase activity and at least one altered property as compared to the parent &agr;-amylase, comprises i) analysing the structure of the parent Termamyl-like &agr;-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like &agr;-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like &agr;-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like &agr;-amylase variant, which as compared to the parent Termamyl-like &agr;-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like &agr;-amylase variant with respect to the property in question.Type: GrantFiled: August 10, 2000Date of Patent: August 27, 2002Assignee: Novozymes A/SInventors: Allan Svendsen, Henrik Bisgård-Frantzen, Torben Vedel Borchert
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Patent number: 6436888Abstract: The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca2+ dependency and specific activity.Type: GrantFiled: September 28, 2000Date of Patent: August 20, 2002Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgård-Frantzen
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Publication number: 20020098996Abstract: The present invention relates to variants of a parent &agr;-amylase, which parent &agr;-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No.Type: ApplicationFiled: July 10, 2001Publication date: July 25, 2002Applicant: Novozymes A/SInventors: Henrik Bisgard-Frantzen, Allan Svendsen, Torben Vedel Borchert
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Patent number: 6368805Abstract: A method for the construction of a library of recombined polynucleotides from a number of different starting single or double stranded parental DNA templates is disclosed, wherein the starting single or double stranded parental DNA templates represent discrete points in a population of genes encoding evolutionary or synthetic homologues of a peptide having homologies ranging over a broad spectrum from less than 15% to more than 80%, said population exhibiting at least one identification sequence, and whereby said genes are subjected to a gene shuffling procedure to generate shuffled mutants of said population of genes representing additional discrete points between those of said starting templates.Type: GrantFiled: August 23, 2000Date of Patent: April 9, 2002Assignee: Novozymes A/SInventors: Torben Vedel Borchert, Titus Kretzschmar, Joel R. Cherry, Vind Jesper
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Patent number: 6361989Abstract: The invention relates to a novel Termamyl-like &agr;-amylase, and Termamyl-like &agr;-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca2+ concentrations (relative to the parent).Type: GrantFiled: April 13, 1999Date of Patent: March 26, 2002Assignee: Novozymes A/SInventors: Allan Svendsen, Torben Vedel Borchert, Henrik Bisgard-Frantzen, Helle Outtrup, Bjarne Ronfeldt Nielsen, Vibeke Skovgaard Nielsen, Lisbeth Hedegaard