Patents by Inventor Toshihiko Ashikari
Toshihiko Ashikari has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7670828Abstract: Provided herein are a method for selecting a gene participating in the desired brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae; and (D) analyzing the selected gene.Type: GrantFiled: August 7, 2006Date of Patent: March 2, 2010Assignee: Suntory Holdings LimitedInventors: Yoshihiro Nakao, Norihisa Nakamura, Yukiko Kodama, Tomoko Fujimura, Toshihiko Ashikari
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Publication number: 20080220503Abstract: Provided herein are a method for selecting a gene participating in the desire brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae; and (D) analyzing the selected gene.Type: ApplicationFiled: August 7, 2006Publication date: September 11, 2008Inventors: Yoshihiro Nakao, Norihisa Nakamura, Yukiko Kodama, Tomoko Fujimura, Toshihiko Ashikari
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Patent number: 7365164Abstract: Provided herein are a method for selecting a gene participating in the desired brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae; and (D) analyzing the selected gene.Type: GrantFiled: August 7, 2006Date of Patent: April 29, 2008Assignee: Suntory LimitedInventors: Yoshihiro Nakao, Norihisa Nakamura, Yukiko Kodama, Tomoko Fujimura, Toshihiko Ashikari
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Publication number: 20070042410Abstract: Provided herein are a method for selecting a gene participating in the desired brewing character and compiling a database of the whole genome sequence of industrial yeast; identifying a gene participating in a brewing characteristic from the database; functional analysis of the gene; and a DNA array of the whole genome sequences of an industrial yeast. Also provided are a method for yeast breeding; a method of producing an alcoholic beverage with improved quality; and a screening method to identify genes that increase productivity and/or improve flavor in the production of an alcohol or an alcoholic beverage by (A) analyzing a whole industrial yeast genome sequence, (B) comparing the genome sequence with the genome sequence of S. cerevisiae, (C) selecting a gene of the industrial yeast encoding having 70 to 97% identity to an amino acid sequence of S. cerevisiae; and (D) analyzing the selected gene.Type: ApplicationFiled: August 7, 2006Publication date: February 22, 2007Inventors: Yoshihiro Nakao, Norihisa Nakamura, Yukiko Kodama, Tomoko Fujimura, Toshihiko Ashikari
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Patent number: 7105719Abstract: The invention relates to isolated polynucleotides encoding a protein having aromatic acyltransferase activity, vectors comprising aromatic acyltransferase polynucleotides, and methods of transforming plants with anthocyanin acyltransferase polynucleotides to alter flower colors. The invention also relates to transformed host cells, plants, and flower cuts expressing proteins having anthocyanin acyltransferase activity.Type: GrantFiled: February 16, 1996Date of Patent: September 12, 2006Assignee: Suntory LimitedInventors: Toshihiko Ashikari, Yoshikazu Tanaka, Hiroyuki Fujiwara, Masahiro Nakao, Yuko Fukui, Keiko Sakakibara, Masako Mizutani, Takaaki Kusumi
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Publication number: 20060099612Abstract: An object of the present invention is to provide a method for analyzing gene of an industrial yeast. The method of the present invention comprises (a) analyzing the genome sequence of the industrial yeast; and (c-1) selecting a gene of the industrial yeast encoding an amino acid sequence having 70 to 97% identity to an amino acid sequence encoded by the gene of Saccharomyces cerevisiae, or (c-2) selecting a gene of the industrial yeast consisting of a nucleotide sequence having 60 to 94% identity to the nucleotide sequence of the gene of Saccharomyces cerevisiae.Type: ApplicationFiled: September 2, 2005Publication date: May 11, 2006Inventors: Yoshihiro Nakao, Norihisa Nakamura, Yukiko Kodama, Tomoko Fujimura, Toshihiko Ashikari
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Publication number: 20060046253Abstract: An object of the present invention is to provide a method for analyzing gene of an industrial yeast. The method of the present invention comprises (a) determining the whole genome sequence of the industrial yeast; and (c-1) selecting a gene of the industrial yeast encoding an amino acid sequence having 70 to 97% identity to an amino acid sequence encoded by the gene of Saccharomyces cerevisiae, or (c-2) selecting a gene of the industrial yeast consisting of a nucleotide sequence having 60 to 94% identity to the nucleotide sequence of the gene of Saccharomyces cerevisiae.Type: ApplicationFiled: September 2, 2004Publication date: March 2, 2006Applicant: SUNTORY LIMITEDInventors: Yoshihiro Nakao, Norihisa Nakamura, Yukiko Kodama, Tomoko Fujimura, Toshihiko Ashikari
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Publication number: 20040265862Abstract: The present invention provides (A) a method for the selection of genes participating in the desired brewing character, which comprises preparing a database compiling the data of the whole genome sequence of industrial yeast, particularly a brewing yeast used for alcoholic beverages; selecting gene participating in a desired brewing character that the brewing yeast specifically possesses; and carrying out functional analysis of the gene by disruption or overexpression; (B) a DNA array in which oligonucleotide(s) selected based on the data base compiling the data of the whole genome sequences of an industrial yeast, (C) a breeding method for constructing improved cultures achieving the desired brewing character, (D) a method for producing an alcohol or an alcoholic beverage in which productivity and quality are improved using the yeast, (E) a gene which is specific to the improved brewing yeast, and (F) a peptide encoded by the gene.Type: ApplicationFiled: March 4, 2004Publication date: December 30, 2004Applicant: SUNTORY LIMITEDInventors: Yoshihiro Nakao, Norihisa Nakamura, Yukiko Kodama, Tomoko Fujimura, Toshihiko Ashikari
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Patent number: 6723562Abstract: A DNA construct comprising: (1) a selective marker gene, (2) a galactose-inducible growth inhibition sequence, (3) a pair of FRT sequences in the same orientation flanking (1) and (2), and (4) a DNA fragment capable of recombining with a yeast chromosomal DNA located at each end of (3), wherein said FRT sequences contain the following sequence: 5′-GAAGTTCCTATAC TTTCTAGA GAATAGGAACTTC-3′ (SEQ ID NO: 1) inverted spacer inverted repeat (1) sequence repeat (2) or a sequence substantially identical to said sequence.Type: GrantFiled: June 25, 2001Date of Patent: April 20, 2004Assignee: Suntory LimitedInventors: Toshihiko Ashikari, Misa Ochiai
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Patent number: 6066484Abstract: Novel purine nucleosidase derived from Ochrobactrum anthropi microorganisms, a gene system coding therefor, uses therefor and particularly its use in the production of beer.Type: GrantFiled: August 18, 1997Date of Patent: May 23, 2000Assignee: Suntory LimitedInventors: Haruyo Hatanaka, Toshihiko Ashikari, Jun Ogawa, Sakayu Shimizu
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Patent number: 5965444Abstract: A DNA construct wherein a DNA fragment which is recombinable in yeast chromosomal DNA is directly or indirectly linked at both ends of a DNA fragment which comprises a pair of R sensitive sequences oriented in the same direction and flaking both an R gene placed under the control of an inducible promoter and an expressible selective marker gene, which is a DNA construct designed with the R sensitive sequences non-symmetrically shortened, so that no functionable R sensitive sequence remains after the R sensitive sequence recombination has occurred by expression of the R gene and the selective marker has been removed. Since no functionable R sensitive sequence remains after removal of the selective marker, recombination does not occur again, and thus the same selective marker may be used for multiple insertions of foreign genes.Type: GrantFiled: June 23, 1997Date of Patent: October 12, 1999Assignee: Suntory LimitedInventors: Toshihiko Ashikari, Hiroto Kondo, Keiko Sakakibara, Hiroyuki Araki, Yasuji Oshima
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Patent number: 5286638Abstract: A peroxidase gene derived from Arthromyse ramosus and its cloning and expression in E. coli and yeast is disclosed. The peroxidase encoded by this gene is not-specific and does not contain isozymes and hence is suitable as a diagnostic reagent or as a marker enzyme in enzyme innunoassays.Type: GrantFiled: November 15, 1991Date of Patent: February 15, 1994Assignee: Suntory Limited of 1-40, DojimahamaInventors: Yoshikazu Tanaka, Toshihiko Ashikari, Haruyo Hatanaka, Yuji Shibano, Teruo Amachi, Toru Nakayama, Motoo Sumida
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Patent number: 5084385Abstract: This invention provides a process for producing ethanol from a starchy material, wherein the starchy material is simultaneously subjected to saccharification and subsequent fermentation by use of a yeast host which has been transformed by an expressible recombinant vector comprising a glucoamylase gene derived from a fungus of the genus Rhizopus.Type: GrantFiled: April 14, 1989Date of Patent: January 28, 1992Assignee: Suntory LimitedInventors: Toshihiko Ashikari, Norihisa Nakamura, Yoshikazu Tanaka, Yuji Shibano, Hajime Yoshizumi
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Patent number: 4863864Abstract: The present invention relates to the Rhizopus derived glucoamylase gene, a novel recombinant vector comprising said gene, and a microorganism transformed by said vector, as well as a process for reproducing Rhizopus glucoamylase by cultivating the transformed microorganism, especially yeast, in a liquid medium.Type: GrantFiled: December 13, 1985Date of Patent: September 5, 1989Assignee: Suntory LimitedInventors: Toshihiko Ashikari, Norihisa Nakamura, Yoshikazu Tanaka, Yuji Shibano, Hajime Yoshizumi