Abstract: An object of the present invention is to provide a method for measuring respiratory sensitization and a respiratory sensitization measuring reagent that can be used to evaluate a test substance for respiratory sensitization without using an animal. According to the present invention, there is provided a method for measuring respiratory sensitization, including reacting a N-(arylalkylcarbonyl)cysteine with a test substance; reacting an ?-N-(arylalkylcarbonyl)lysine with the test substance; detecting the amount of each of the above compounds or a product thereof after the reaction by optical measurement; and determining respiratory sensitization from the ratio of the reactivity of the ?-N-(arylalkylcarbonyl)lysine with the test substance to the reactivity of the N-(arylalkylcarbonyl)cysteine with the test substance or from the reactivity of the ?-N-(arylalkylcarbonyl)lysine with the test substance.

Abstract: A method for measuring phototoxicity or photoallergy includes reacting a test substance with an organic compound that is an N-(arylalkylcarbonyl)cysteine or an ?-N-(arylalkylcarbonyl)lysine under irradiation with ultraviolet light; reacting the test substance with the organic compound without irradiation with ultraviolet light; determining the depletion of the organic compound after each reaction by an optical measurement; and detecting phototoxicity or photoallergy from the difference between the depletion of the organic compound after the reaction under irradiation with ultraviolet light and the depletion of the organic compound after the reaction without irradiation with ultraviolet light.

Abstract: An object of the present invention is to provide a skin sensitization measuring reagent, a method for measuring skin sensitization, and a compound that allow the sensitization of a test substance to be measured using a single reagent. According to the present invention, there is provided a skin sensitization measuring reagent including, as a main measuring agent, an organic compound having at least one thiol group derived from an amino acid and at least one amino group derived from a side chain of an amino acid. The organic compound has an absorption spectrum in an ultraviolet, visible, or near-infrared region and has a molar absorption coefficient of 100 L/mol·cm or more and 500,000 L/mol·cm or less at a maximal absorption wavelength.

Abstract: An object of the present invention is to provide a method for determining skin allergenicity of a mixture containing two or more test substances, and a reagent for determining the skin allergenicity. The present invention provides a method for determining skin allergenicity, the method including causing a reaction between an organic compound that has at least one thiol group or amino group and emits fluorescence, and a mixture containing two or more test substances, and determining an amount of the organic compound after the reaction or an amount of a product of the reaction by an optical measurement using a fluorescence detector; and a reagent for determining skin allergenicity, the reagent including, as a measurement basis, an organic compound that has at least one thiol group or amino group and emits fluorescence, and being used for determining skin allergenicity of a mixture containing two or more test substances by an optical measurement using a fluorescence detector.