Patents by Inventor Toshihiko Kuroda
Toshihiko Kuroda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9944976Abstract: A method of stirring a test substance-containing solution injected into an analysis chip, wherein said analysis chip includes a recess to which said test substance-containing solution is injected; and a selective binding substance, which selectively binds to said test substance, is immobilized on the entirety or a part of the bottom surface of said recess, said method including injecting said test substance-containing solution to the space in said recess of said analysis chip such that said space is partially left unfilled; and rotating said analysis chip to which said test substance-containing solution is injected applying a centrifugal acceleration of not less than 1×g.Type: GrantFiled: February 27, 2013Date of Patent: April 17, 2018Assignee: Toray Industries, Inc.Inventors: Toshihiko Kuroda, Hitoshi Nobumasa
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Patent number: 9206472Abstract: A method of analyzing RNA extracted from a tissue or cell(s) fixed with a fixative, said method comprising a determining step of whether said RNA satisfies: B/A?1 wherein A represents the weight ratio (%) of RNA within the range of 1000 to 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis; and B represents the weight ratio (%) of RNA within the range of more than 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis.Type: GrantFiled: December 15, 2010Date of Patent: December 8, 2015Assignee: Toray Industries, Inc.Inventors: Toshihiko Kuroda, Osamu Nomura, Hitoshi Nobumasa
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Publication number: 20150045250Abstract: A method of stirring a test substance-containing solution injected into an analysis chip, wherein said analysis chip includes a recess to which said test substance-containing solution is injected; and a selective binding substance, which selectively binds to said test substance, is immobilized on the entirety or a part of the bottom surface of said recess, said method including injecting said test substance-containing solution to the space in said recess of said analysis chip such that said space is partially left unfilled; and rotating said analysis chip to which said test substance-containing solution is injected applying a centrifugal acceleration of not less than 1×g.Type: ApplicationFiled: February 27, 2013Publication date: February 12, 2015Inventors: Toshihiko Kuroda, Hitoshi Nobumasa
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Publication number: 20120253687Abstract: A method of analyzing RNA extracted from a tissue or cell(s) fixed with a fixative, said method comprising a determining step of whether said RNA satisfies: B/A?1 wherein A represents the weight ratio (%) of RNA within the range of 1000 to 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis; and B represents the weight ratio (%) of RNA within the range of more than 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis.Type: ApplicationFiled: December 15, 2010Publication date: October 4, 2012Applicant: TORAY INDUSTRIES, INC.Inventors: Toshihiko Kuroda, Osamu Nomura, Hitoshi Nobumasa
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Publication number: 20120196768Abstract: A method for preparing aRNA to be used for gene expression analysis from an RNA sample extracted from a tissue or cell(s) fixed with a fixative includes an amplification step of the RNA sample by reverse transcription and in vitro transcription, the ratio of aminoallyl uridine 5?-triphosphate (AA-UTP) in a nucleotide reagent used in the in vitro transcription is not less than 5 mol % and less than 25 mol % with respect to the total of uridine 5?-triphosphate (UTP) and AA-UTP.Type: ApplicationFiled: April 21, 2011Publication date: August 2, 2012Applicant: TORAY INDUSTRIES, INC.Inventors: Toshihiko Kuroda, Osamu Nomura, Hitoshi Nobumasa
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Patent number: 7795010Abstract: The following is disclosed: (1) a membrane fractionator including a filtration section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; (2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system that in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human alpha1-microglobulin and human albumin of 1.Type: GrantFiled: January 20, 2005Date of Patent: September 14, 2010Assignee: Toray Industries, Inc.Inventors: Kazuhiro Tanahashi, Ichiro Kumo, Nobuyuki Kuroki, Hiroyuki Sugaya, Satoko Yamada, Shigehisa Wada, Giman Jung, Toshihiko Kuroda, Shuji Sekiguchi
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Publication number: 20100029503Abstract: This invention is directed to an analysis chip comprising a substrate having a surface on which a selective binding substance is immobilized; a cover member adhered to the substrate; and particles movably contained or injected in a void between the substrate and the cover member; wherein the surfaces of the particles are coated with a surfactant. By this invention, generation of bubbles which inhibit the selective reaction between the test substance and the immobilized selective binding substance is suppressed, thereby reducing the deviation of data, suppressing the lowering of sensitivity, and promoting the reproducibility of the measurement.Type: ApplicationFiled: January 23, 2008Publication date: February 4, 2010Applicant: TORAY INDUSTRIES, INC.Inventors: Osamu Nomura, Toshihiko Kuroda, Hitoshi Nobumasa, Yasuo Murao
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Publication number: 20070244306Abstract: The following is disclosed: (1) a membrane fractionator including a filtration section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; (2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system that in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human alpha1-microglobulin and human albumin of 1.Type: ApplicationFiled: January 20, 2005Publication date: October 18, 2007Inventors: Kazuhiro Tanahashi, Ichiro Kumo, Nobuyuki Kuroki, Hiroyuki Sugaya, Satoko Yamada, Shigehisa Wada, Giman Jung, Toshihiko Kuroda, Shuji Sekiguchi
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Patent number: 4363005Abstract: A power source circuit for a discharge type sensing circuit, comprising a blocking oscillator (1 ) and a feedback circuit (2) for changing the period of oscillation of the blocking oscillator (1). The sensing circuit as a load is connected to a secondary winding (L.sub.2) of a transformer (T) of the blocking oscillator (1). The base bias of a transistor (Tr.sub.1) of the blocking oscillator is controlled by the feedback circuit (2) to which is applied a negative voltage produced in the primary winding (L.sub.1) in an off state of the transistor (Tr.sub.1). As a result, when the sensing circuit is in the sensing mode of operation, the period of oscillation becomes shorter for rapid supply of electric power and, when in the waiting mode of operation, the period of oscillation becomes longer for reducing supply of power, so that the power consumption is reduced as a whole.Type: GrantFiled: February 27, 1981Date of Patent: December 7, 1982Assignee: Dentan Kabushiki KaishaInventors: Toshihiko Kuroda, Kazuo Sugiyama