Patents by Inventor Toshihiro Kuroita
Toshihiro Kuroita has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7601504Abstract: It is intended to provide a method of conveniently screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; and a protein achieving an improved blocking efficiency that can be expressed on a large scale in Escherichia coli. A method of screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; a protein characterized by achieving an improved blocking efficiency owing to an amino acid sequence modification; and a method of utilizing the protein.Type: GrantFiled: July 2, 2004Date of Patent: October 13, 2009Assignee: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Atsushi Sogabe, Yutaka Takarada, Naoki Tanaka
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Patent number: 7422882Abstract: An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3?-5? exonuclease activity, histidine(H) has been replaced by another amino acid.Type: GrantFiled: May 10, 2001Date of Patent: September 9, 2008Assignee: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Masao Kitabayashi, Yoshikazu Ishida, Hideyuki Komatsubara, Yoshiaki Nishiya, Bunsei Kawakami, Yoshihisa Kawamura, Tadayuki Imanaka
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Patent number: 7384739Abstract: The invention provides methods, kits, and compositions for enhancing synthesis of DNA involving a carboxylate ion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions. The invention further provides a thermostable DNA polymerase-related factor derived from Thermococcus species, which has an activity to promote the DNA synthesis activity of DNA polymerase or which binds to DNA polymerase.Type: GrantFiled: November 14, 2002Date of Patent: June 10, 2008Assignee: Toyo Boseki Kabushiki KaishaInventors: Masao Kitabayashi, Toshihiro Kuroita, Yoshikazu Ishida, Hideyuki Komatsubara, Yoshiaki Nishiya, Masanori Oka, Yoshihisa Kawamura, Tadayuki Imanaka
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Publication number: 20060183173Abstract: It is intended to provide a method of conveniently screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; and a protein achieving an improved blocking efficiency that can be expressed on a large scale in Escherichia coli. A method of screening a novel protein or a novel partial sequence protein having a blocking ability based on amino acid sequence data; a protein characterized by achieving an improved blocking efficiency owing to an amino acid sequence modification; and a method of utilizing the protein.Type: ApplicationFiled: July 2, 2004Publication date: August 17, 2006Applicant: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Atsushi Sogabe, Yutaka Takarada, Naoki Tanaka
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Patent number: 7048915Abstract: The present invention provides a composition for cell-free protein synthesis, which is superior in storage stability in a freeze-dried state, more particularly a freeze-dryable or freeze-dried composition for cell-free protein synthesis, which contains a cell extract for cell-free protein synthesis and inositol, and a freeze-dryable or freeze-dried composition for cell-free protein synthesis containing a cell extract for cell-free protein synthesis, and a deliquescent material in a proportion of not more than 0.01 part by weight per part by weight of a protein in the composition; and a composition for cell-free protein synthesis superior in storage stability in a frozen state, more particularly a freezable or frozen composition for cell-free protein synthesis, containing a cell extract for cell-free protein synthesis and polyhydric alcohol.Type: GrantFiled: April 18, 2002Date of Patent: May 23, 2006Assignee: CellFree Sciences Co., Ltd.Inventors: Toshihiro Kuroita, Bunsei Kawakami, Yoshihisa Kawamura, Shigemichi Nishikawa, Yaeta Endo
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Publication number: 20050069887Abstract: A composition for enhancing synthesis of DNA comprising an anion-supplying substance that is effective in promoting DNA synthesis in enzymatic DNA synthesis reactions, in particular a salt of a dicarboxylic acid. Further enhanced DNA synthesis promoting effects can be achieved by using, together with the anion-supplying substance, at least one compound selected from the group consisting of dimethylsulfoxide and compounds represented by the following formula (1): R2—CH2—NR1xHy??(1) wherein R1 is an alkyl group having 1 to 3 carbon atoms, R2 is a substituent selected from the group consisting of the following (a) and (b): (a) oxygen and (b) radicals represented by the formula wherein R4 is methyl, hydrogen or forms a pyrrolidine ring when combined with R1, R5 is —CO2H or —SO3H, and n is an integer from 0 to 2, x is an integer from 1 to 3 and y is an integer from 0 to 2, provided that x plus y equals 3.Type: ApplicationFiled: November 14, 2002Publication date: March 31, 2005Inventors: Masao Kitabayashi, Toshihiro Kuroita, Yoshikazu Ishida, Hideyuki Komatsubara, Yoshiaki Nishiya, Masanori Oka, Yoshihisa Kawamura, Tadayuki Imanaka
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Publication number: 20030199076Abstract: The present invention provides a composition for cell-free protein synthesis, which is superior in storage stability in a freeze-dried state, more particularly a freeze-dryable or freeze-dried composition for cell-free protein synthesis, which contains a cell extract for cell-free protein synthesis and inositol, and a freeze-dryable or freeze-dried composition for cell-free protein synthesis containing a cell extract for cell-free protein synthesis, and a deliquescent material in a proportion of not more than 0.01 part by weight per part by weight of a protein in the composition; and a composition for cell-free protein synthesis superior in storage stability in a frozen state, more particularly a freezable or frozen composition for cell-free protein synthesis, containing a cell extract for cell-free protein synthesis and polyhydric alcohol.Type: ApplicationFiled: April 18, 2002Publication date: October 23, 2003Applicants: Toyo Boseki Kabushiki Kaisha, WAKENYAKU CO., LTD., Yaeta EndoInventors: Toshihiro Kuroita, Bunsei Kawakami, Yoshihisa Kawamura, Shigemichi Nishikawa, Yaeta Endo
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Publication number: 20020076768Abstract: An object of the present invention is to provide a thermostable DNA polymerase with enhanced amplification efficiency and/or improved fidelity in polymerase chain reaction (PCR), and provide a process for production thereof. More specifically, the present invention provides thermostable DNA polymerase wherein in the DX1EX2X3X4H sequence (D: aspartic acid, E: glutamic acid, H: histidine, X1, X2, X3 and X4: any amino acid) consisting of DX1E sequence within the EXO I region and a four amino acid length peptide adjacent to said glutamic acid(E) of thermostable DNA polymerase having 3′-5′ exonuclease activity, histidine(H) has been replaced by another amino acid.Type: ApplicationFiled: May 10, 2001Publication date: June 20, 2002Applicant: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Masao Kitabayashi, Yoshikazu Ishida, Hideyuki Komatsubara, Yoshiaki Nishiya, Bunsei Kawakami, Yoshihisa Kawamura, Tadayuki Imanaka
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Patent number: 5990302Abstract: A method for isolating a ribonucleic acid, which comprises dissolution of a sample containing the ribonucleic acid, such as cells, in an acidic solution containing a lithium salt and a chaotropic agent, bringing the ribonucleic acid into contact with a nucleic acid-binding carrier such as silica particles, thereby to allow selective adsorption of the ribonucleic acid alone onto said carrier, and eluting the ribonucleic acid from the nucleic acid-bound carrier; a reagent therefor; and a method for producing a cDNA from the ribonucleic acid isolated by this method. According to the present invention, a high purity ribonucleic acid can be isolated quickly and safely from a sample containing the ribonucleic acid.Type: GrantFiled: July 11, 1997Date of Patent: November 23, 1999Assignee: Toyo Boseki Kabushiki KaishaInventors: Toshihiro Kuroita, Hideki Kamimura, Bunsei Kawakami, Yoshihisa Kawamura