Abstract: There is provided a novel method for culturing a hepatic epithelioid tissue. The method uses a method for forming a hepatic epithelioid tissue having a structure of connections between hepatocytes and bile duct epithelial cells, comprising a step of culturing bile duct epithelial cells on a collagen gel, and a step of inoculating and culturing hepatocytes on the cultured bile duct epithelial cells.

Abstract: An image processing device can explicitly distinguish cells to be observed from cells other than those to be observed with a simple configuration. To this end, the image processing device includes a color information obtaining part obtaining at least hue from color information of each pixel of a color image, a detecting part detecting a mode value of the hue on a color space, a range setting part setting a predetermined range on the color space including the mode value of the hue detected by the detecting part as a target range, a changing part changing hue of a pixel included in the target range by virtually performing extension on the target range, and an information converting part converting color information of a pixel having hue not included in the target range into color information indicative of an achromatic color.

Abstract: An inspection apparatus includes an imaging device and an image processing device. The imaging device photographs a specimen and outputs a color image of the specimen to the image processing device. The image processing device subjects the color image of the specimen to negative-positive reversal. After detecting a mode value of the hue the color image having been subjected to negative-positive reversal, the image processing device changes the hue of the color image in accordance with a difference between a boundary value of two predefined hues and the detected mode value. In accordance with the change of the hue, a plurality of target pixels different in the saturation is extracted and the saturation and the intensity of each pixel are changed, or the gradation of each pixel is converted so that the plurality of target pixels becomes most distant from one another in a color space.

Abstract: An image processing device can explicitly distinguish cells to be observed from cells other than those to be observed with a simple configuration. To this end, the image processing device includes a color information obtaining part obtaining at least hue from color information of each pixel of a color image, a detecting part detecting a mode value of the hue on a color space, a range setting part setting a predetermined range on the color space including the mode value of the hue detected by the detecting part as a target range, a changing part changing hue of a pixel included in the target range by virtually performing extension on the target range, and an information converting part converting color information of a pixel having hue not included in the target range into color information indicative of an achromatic color.

Abstract: An inspection apparatus includes an imaging device and an image processing device. The imaging device photographs a specimen and outputs a color image of the specimen to the image processing device. The image processing device subjects the color image of the specimen to negative-positive reversal. After detecting a mode value of the hue the color image having been subjected to negative-positive reversal, the image processing device changes the hue of the color image in accordance with a difference between a boundary value of two predefined hues and the detected mode value. In accordance with the change of the hue, a plurality of target pixels different in the saturation is extracted and the saturation and the intensity of each pixel are changed, or the gradation of each pixel is converted so that the plurality of target pixels becomes most distant from one another in a color space.

Abstract: An inspection apparatus includes an imaging device and an image processing device. The imaging device photographs a specimen and outputs a color image of the specimen to the image processing device. The image processing device subjects the color image of the specimen to negative-positive reversal. After detecting a mode value of the hue of the color image having been subjected to negative-positive reversal, the image processing device changes the hue of the color image in accordance with a difference between a boundary value of two predefined hues and the detected mode value. In accordance with the change of the hue, a plurality of target pixels different in the saturation is extracted and the saturation and the intensity of each pixel are changed, or the gradation of each pixel is converted so that the plurality of target pixels becomes most distant from one another in a color space.

Abstract: Cultured cells flat-cultured on a permeable sheet is stacked on other flat-cultured cells together with the permeable sheet to construct a three-dimensional tissue. The three-dimensional tissue is transplanted into a living body. Alternatively, the three-dimensional tissue is stacked up in an artificial organ device to construct a stacked-up, three-dimensional bioartificial organ module. Colony form of the cultured cell can be controlled by using a microporous sheet as the permeable sheet and controlling positions of pores in the microporous sheet to design the form of the artificial organ.

Abstract: A method of culturing small hepatocytes is provided, which method comprises culturing the small hepatocytes in the presence of a carrier of which surface is attached by hyaluronic acid (hyaluronic acid-attached carrier) and/or in the presence of a carrier of which a major component is hyaluronic acid. Additionally, a method of culturing small hepatocytes is also provided, which method comprises culturing the small hepatocytes in the presence of a carrier of which surface is attached by hyaluronic acid and/or in the presence of a carrier of which a major component is hyaluronic acid, and separating cells which adhere to hyaluronic acid from cells which do not adhere to hyaluronic acid.

Abstract: The present invention provides a process for preparing a transplantable liver tissue, cell colonies suitable for the process, and a process for preparing them. At least about 70 % of the cells in the small hepatocyte-rich colonies of the present invention are small hepatocytes. In particular, each colony comprises about 10 to 30 cells and the number of the small hepatocytes is at least about 70 % based on the total number of the cells in the colony. A process for preparing such small hepatocyte-rich colonies is also disclosed. The present invention also discloses a process for inducing the maturation of the small hepatocyte-rich colonies into a liver tissue and a method of estimating the effect of a drug, particularly the effect thereof connected with normal liver function in vitro, using the cell colonies enriched with the small hepatocytes which have been induced to be matured.

Abstract: The present invention provides a process for preparing a transplantable liver tissue, cell colonies suitable for the process, and a process for preparing them. At least about 70% of the cells in the small hepatocyte-rich colonies of the present invention are small hepatocytes. In particular, each colony comprises about 10 to 30 cells and the number of the small hepatocytes is at least about 70% based on the total number of the cells in the colony. A process for preparing such small hepatocyte-rich colonies is also disclosed. The present invention also discloses a process for inducing the maturation of the small hepatocyte-rich colonies into a liver tissue and a method of estimating the effect of a drug, particularly the effect thereof connected with normal liver function in vitro, using the cell colonies enriched with the small hepatocytes which have been induced to be matured.

Abstract: A method of preparing small hepatocytes is provided. The method is suitable for cryopreservation wherein the hepatic function and proliferation ability of the small hepatocytes is retained. A method of cryopreserving thus prepared small hepatocytes and the cryopreserved small hepatocytes having hepatic functions and proliferation ability are also provided. According to the present invention, small hepatocytes are cultured using a medium supplemented with nicotinamide to form colonies of small hepatocytes in which the small hepatocytes are encompassed by nonparenchymal cells, and then the formed colonies are dissociated from culture dishes as small hepatocytes aggregate by non-enzymatic treatment, suspended in a cryopreservation solution and are cryopreserved.