Patents by Inventor Toshihiro Shikakura

Toshihiro Shikakura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • PEPTIDE COMPLEX AND PRODUCTION METHOD THEREFOR, AND USE OF SAID PEPTIDE COMPLEX
    Publication number: 20220162259
    Abstract: A peptide complex including: a peptide; and a cell-membrane-permeability-imparting group introduced into the peptide, wherein the cell-membrane-permeability-imparting group includes a dendritic structure including four or more branches, and at least four branches of the four or more branches include basic functional groups at ends thereof.
    Type: Application
    Filed: February 14, 2020
    Publication date: May 26, 2022
    Applicant: KANEKA CORPORATION
    Inventors: Keishi TAKATSU, Toshihiro SHIKAKURA, Yuka MATSUDA, Tatsuya MOUTAI, Hirofumi MAEDA, Hiroshi KITA, Mitsuaki KITANO
  • Ribosome display complex and production method therefor
    Patent number: 11248226
    Abstract: A method for producing a ribosome display complex includes obtaining a ribosome complex including an unmodified polypeptide chain, an mRNA molecule and a ribosome by initiating translation of the mRNA molecule in a cell-free peptide synthesis system including the ribosome, and modifying the unmodified polypeptide chain by reacting a side chain reactive functional group in the unmodified polypeptide chain with a modifying reagent to produce a ribosome display complex including a modified polypeptide chain, the mRNA molecule and the ribosome. The unmodified polypeptide chain includes at least one reactive amino acid residue selected from the group consisting of a cysteine residue, a lysine residue, a histidine residue and a tryptophan residue. The at least one reactive amino acid residue includes the side chain reactive functional group, and the mRNA molecule includes a base sequence encoding an amino acid sequence of the polypeptide chain.
    Type: Grant
    Filed: December 7, 2018
    Date of Patent: February 15, 2022
    Assignee: KANEKA CORPORATION
    Inventors: Hiroshi Kita, Toshihiro Shikakura, Hirofumi Maeda, Keishi Takatsu
  • RIBOSOME DISPLAY COMPLEX AND PRODUCTION METHOD THEREFOR
    Publication number: 20190169600
    Abstract: A method for producing a ribosome display complex includes obtaining a ribosome complex including an unmodified polypeptide chain, an mRNA molecule and a ribosome by initiating translation of the mRNA molecule in a cell-free peptide synthesis system including the ribosome, and modifying the unmodified polypeptide chain by reacting a side chain reactive functional group in the unmodified polypeptide chain with a modifying reagent to produce a ribosome display complex including a modified polypeptide chain, the mRNA molecule and the ribosome. The unmodified polypeptide chain includes at least one reactive amino acid residue selected from the group consisting of a cysteine residue, a lysine residue, a histidine residue and a tryptophan residue. The at least one reactive amino acid residue includes the side chain reactive functional group, and the mRNA molecule includes a base sequence encoding an amino acid sequence of the polypeptide chain.
    Type: Application
    Filed: December 7, 2018
    Publication date: June 6, 2019
    Applicant: KANEKA CORPORATION
    Inventors: Hiroshi Kita, Toshihiro Shikakura, Hirofumi Maeda, Keishi Takatsu