Patents by Inventor Toshihiro Yonekawa
Toshihiro Yonekawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200032241Abstract: A method for extracting nucleic acid from a biological sample is disclosed. The method comprises steps of: (i) mixing a biological sample, an anionic surfactant and a chaotropic compound to obtain a lysate; (ii) mixing the lysate, an alkanol having a boiling point higher than 75° C., a chaotropic compound and silica particles to adsorb the nucleic acid on the silica particles; (iii) washing the silica particles having the nucleic acid adsorbed thereon with a washing liquid; and (iv) eluting the nucleic acid adsorbed on the silica particles with an eluent, wherein at least steps (i) and (ii) are performed at a temperature higher than 75° C.Type: ApplicationFiled: September 19, 2017Publication date: January 30, 2020Inventors: Masaki SATO, Toshihiro YONEKAWA
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Publication number: 20190302137Abstract: The present invention aims to provide a compact, fully automatic genetic testing device with which a step of extracting and purifying a nucleic acid from a sample, a step of injecting a solution containing the nucleic acid, and a step of amplifying and detecting the nucleic acid, can be fully automatically carried out, and which enables a physician to carry out genetic testing at a position close to a patient. The fully automatic genetic testing device comprises: a first unit for extracting and purifying a nucleic acid from a sample; a second unit for injecting a solution containing the nucleic acid to a multi-well chip having a reaction site for amplification of the nucleic acid; and a third unit for optically detecting the nucleic acid amplified in the reaction site(s).Type: ApplicationFiled: March 29, 2018Publication date: October 3, 2019Inventors: Yuji SEGAWA, Hidetoshi WATANABE, Toshihiro YONEKAWA, Fumihiko KAZUMI, Rod PETERSON, Anatoly MOSKALEV, Goran FORSHAGER, James FOSTER
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Publication number: 20140308663Abstract: The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.Type: ApplicationFiled: June 27, 2013Publication date: October 16, 2014Inventors: Toshihiro YONEKAWA, Tsugunori NOTOMI, Hidetoshi KANDA, Norimitsu HOSAKA
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Patent number: 8557523Abstract: The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.Type: GrantFiled: October 17, 2008Date of Patent: October 15, 2013Assignee: Eiken Kagaku Kabushiki KaishaInventors: Toshihiro Yonekawa, Tsugunori Notomi, Hidetoshi Kanda, Norimitsu Hosaka
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Patent number: 8389221Abstract: The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis.Type: GrantFiled: September 14, 2011Date of Patent: March 5, 2013Assignees: Japan as represented by Director-General of National Institute of Infectious Diseases, Eiken Kagaku Kabushiki KaishaInventors: Harumi Minekawa, Toshihiro Yonekawa, Takato Odagiri
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Publication number: 20120100527Abstract: The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis.Type: ApplicationFiled: September 14, 2011Publication date: April 26, 2012Applicants: JAPAN AS REP. BY NAT'L INST. OF INFECT. DISEASES, EIKEN KAGAKU KABUSHIKI KAISHAInventors: Harumi Minekawa, Tsugunori Notomi, Toshihiro Yonekawa, Norihiro Tomita, Yoko Kuzuhara, Takato Odagiri
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Patent number: 8043813Abstract: The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis.Type: GrantFiled: October 26, 2005Date of Patent: October 25, 2011Assignees: Japan as represented by Director-General of National Institute of Infectious Diseases, Eiken Kagaku Kabushiki KaishaInventors: Harumi Minekawa, Tsugunori Notomi, Toshihiro Yonekawa, Norihiro Tomita, Yoko Kuzuhara, Takato Odagiri
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Publication number: 20100233715Abstract: The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.Type: ApplicationFiled: October 17, 2008Publication date: September 16, 2010Applicant: Eiken Kagaku Kabushiki KaishaInventors: Toshihiro Yonekawa, Tsugunori Notomi, Hidetoshi Kanda, Norimitsu Hosaka
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Patent number: 7638280Abstract: The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3?-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.Type: GrantFiled: December 22, 2006Date of Patent: December 29, 2009Assignee: Eiken Kagaku Kabushiki KaishaInventors: Hidetoshi Kanda, Tsugunori Notomi, Kentaro Nagamine, Toshihiro Yonekawa
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Publication number: 20090317795Abstract: The present invention provides oligonucleotide primers specifically hybridizing to an arbitrary nucleotide sequence designed from the nucleotide sequence of hemagglutinin of an H5 or H7 avian influenza virus, a nucleic acid amplification method using the primers, a method for diagnosis of infection with an H5 or H7 avian influenza virus by detection of nucleic acid amplification, and a kit for influenza diagnosis.Type: ApplicationFiled: October 26, 2005Publication date: December 24, 2009Inventors: Harumi Minekawa, Tsugunori Notomi, Toshihiro Yonekawa, Norihiro Tomita, Yoko Kuzuhara, Takato Odagiri
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Publication number: 20070238113Abstract: The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3?-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.Type: ApplicationFiled: December 22, 2006Publication date: October 11, 2007Applicant: EIKEN KAGAKU KABUSHIKI KAISHAInventors: Hidetoshi Kanda, Tsugunori Notomi, Kentaro Nagamine, Toshihiro Yonekawa
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Patent number: 7175985Abstract: The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3?-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.Type: GrantFiled: August 2, 2002Date of Patent: February 13, 2007Assignee: Eiken Kagaku Kabushiki KaishaInventors: Hidetoshi Kanda, Tsugunori Notomi, Kentaro Nagamine, Toshihiro Yonekawa
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Patent number: 5922544Abstract: A method and a reagent for detecting cells of a living organism contained in various samples of the organism by detecting genes specific for each animal species. This method is useful for detecting bleeding into feces. It is possible for confirming the bleeding of the human body to use an Alu sequence as the sequence specific for the animal species. As genes are used as the indicator in this method, fecal occult blood can be detected more specifically at a higher sensitivity than by the conventional technique using hemoglobin as the indicator.Type: GrantFiled: February 9, 1998Date of Patent: July 13, 1999Assignee: Eiken Kagaku Kabushiki KaishInventors: Kiyoshi Miyai, Tsutomu Naitoh, Toshihiro Yonekawa