Patents by Inventor Toshiki Mine

Toshiki Mine has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • NOVEL ENZYME PROTEIN, PROCESS FOR PRODUCTION OF THE ENZYME PROTEIN THE SAME, AND GENE ENCODING THE ENZYME PROTEIN THE SAME
    Publication number: 20130122566
    Abstract: The present invention relates to a protein having a ?-galactoside-?2,3-sialyltransferase activity but substantially not having any neuraminidase activity, a nucleic acid encoding the enzyme protein, and a method of producing the enzyme using a microorganism transformed with a gene encoding the protein. The protein of the present invention is a modified-type enzyme protein produced by mutating a specific amino acid residue in a ?-galactoside-?2,3-sialyltransferase protein derived from a microorganism belonging to the genus Photobacterium of the family Vibrionaceae and thereby substantially inactivating the neuraminidase activity of the protein.
    Type: Application
    Filed: July 28, 2011
    Publication date: May 16, 2013
    Applicant: JAPAN TOBACCO INC.
    Inventors: Toshiki Mine, Takeshi Yamamoto, Sakurako Katayama
  • ?-galactoside-?2,6-sialyltransferase, a gene encoding thereof, and a method for enhancing enzyme activity
    Patent number: 8372617
    Abstract: The present invention provides an extremely useful and novel ?-galactoside-?2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase.
    Type: Grant
    Filed: April 25, 2012
    Date of Patent: February 12, 2013
    Assignee: Japan Tobacco Inc.
    Inventors: Takeshi Yamamoto, Yoshimitsu Takakura, Toshiki Mine, Yoko Hamada, Hitomi Kajiwara, Masako Ichikawa, Hiroshi Tsukamoto
  • NOVEL BETA-GALACTOSIDE-ALPHA2,6-SIALYLTRANSFERASE, A GENE ENCODING THEREOF, AND A METHOD FOR ENHANCING ENZYME ACTIVITY
    Publication number: 20120282659
    Abstract: The present invention provides an extremely useful and novel ?-galactoside-?2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase.
    Type: Application
    Filed: April 25, 2012
    Publication date: November 8, 2012
    Inventors: Takeshi Yamamoto, Yoshimitsu Takakura, Toshiki Mine, Yoko Hamada, Hitomi Kajiwara, Masako Ichikawa, Hiroshi Tsukamoto
  • Cosmid vector for plant transformation and use thereof
    Patent number: 8298819
    Abstract: The present invention aims to provide novel vectors for plant transformation. The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E.
    Type: Grant
    Filed: June 25, 2007
    Date of Patent: October 30, 2012
    Assignee: Japan Tobacco Inc.
    Inventors: Yoshimitsu Takakura, Toshihiko Komari, Yuji Ishida, Toshiyuki Komori, Yukoh Hiei, Toshiki Mine, Teruyuki Imayama
  • NOVEL PROTEIN AND GENE THAT CODES THEREFOR
    Publication number: 20120184016
    Abstract: The present invention provides a novel protein having neuraminidase activity and/or ?-galactoside-?2,6-sialyltransferase activity and a nucleic acid encoding the protein. The present invention further provides a vector containing a nucleic acid encoding the protein, a host cell transformed with the vector, together with a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase. The present invention also provides an antibody specifically recognizing the protein.
    Type: Application
    Filed: June 11, 2010
    Publication date: July 19, 2012
    Inventors: Toshiki Mine, Takeshi Yamamoto, Hitomi Kajiwara, Hiroshi Tsukamoto
  • ?-galactoside-?2,6-sialyltransferase, a gene encoding thereof, and a method for enhancing enzyme activity
    Patent number: 8187853
    Abstract: The present invention provides an extremely useful and novel ?-galactoside-?2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase.
    Type: Grant
    Filed: March 3, 2008
    Date of Patent: May 29, 2012
    Assignee: Japan Tobacco Inc.
    Inventors: Takeshi Yamamoto, Yoshimitsu Takakura, Toshiki Mine, Yoko Hamada, Hitomi Kajiwara, Masako Ichikawa, Hiroshi Tsukamoto
  • ?-Galactoside-?2, 6-sialyltransferase, a gene encoding thereof, and a method for producing thereof
    Patent number: 8187838
    Abstract: The present invention provides a novel ?-galactoside-?2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase.
    Type: Grant
    Filed: May 27, 2010
    Date of Patent: May 29, 2012
    Assignee: Japan Tobacco Inc.
    Inventors: Hiroshi Tsukamoto, Toshiki Mine, Yoshimitsu Takakura, Takeshi Yamamoto
  • ?-galactoside-?2,6-sialyltransferase, a gene encoding thereof, and a method for producing thereof
    Patent number: 7993875
    Abstract: The present invention provides a novel ?-galactoside-?2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase.
    Type: Grant
    Filed: August 10, 2006
    Date of Patent: August 9, 2011
    Assignee: Japan Tobacco Inc.
    Inventors: Hiroshi Tsukamoto, Toshiki Mine, Yoshimitsu Takakura, Takeshi Yamamoto
  • NOVEL BETA-GALACTOSIDE-ALPHA2, 6-SIALYLTRANSFERASE, A GENE ENCODING THEREOF, AND A METHOD FOR PRODUCING THEREOF
    Publication number: 20100304460
    Abstract: The present invention provides a novel ?-galactoside-?2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase.
    Type: Application
    Filed: May 27, 2010
    Publication date: December 2, 2010
    Inventors: Hiroshi TSUKAMOTO, Toshiki Mine, Yoshimitsu Takakura, Takeshi Yamamoto
  • Cosmid Vector For Plant Transformation And Use Thereof
    Publication number: 20100132068
    Abstract: The present invention aims to provide novel vectors for plant transformation. The vectors of the present invention are cosmid vectors having a full length of 15 kb or less characterized in that: 1) they contain an origin of replication of an IncP plasmid, but do not contain any origin of replication of other plasmid groups; 2) they contain the trfA1 gene of an IncP plasmid; 3) they contain an oriT of an IncP plasmid; 4) they contain the incC1 gene of an IncP plasmid; 5) they contain a cos site of lambda phage and the cos site is located outside the T-DNA; 6) they contain a drug resistance gene expressed in E.
    Type: Application
    Filed: June 25, 2007
    Publication date: May 27, 2010
    Applicant: JAPAN TOBACCO INC.
    Inventors: Yoshimitsu Takakura, Toshihiko Komari, Yuji Ishida, Toshiyuki Komori, Yukoh Hiei, Toshiki Mine, Teruyuki Imayama
  • NOVEL BETA-GALACTOSIDE-a2,6-SIALYLTRANSFERASE, A GENE ENCODING THEREOF, AND A METHOD FOR ENHANCING ENZYME ACTIVITY
    Publication number: 20100112616
    Abstract: The present invention provides an extremely useful and novel ?-galactoside-?2,6-sialyltransferase having an optimum reaction pH in a neutral to alkaline range, and a nucleic acid encoding the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase.
    Type: Application
    Filed: March 3, 2008
    Publication date: May 6, 2010
    Applicant: JAPAN TOBACCO INC.
    Inventors: Takeshi Yamamoto, Yoshimitsu Takakura, Toshiki Mine, Yoko Hamada, Hitomi Kajiwara, Masako Ichikawa, Hiroshi Tsukamoto
  • Novel Beta-Galactoside Alpha 2,6-Sialyltransferase, Gene Coding For The Transferase And Process For Producing The Same
    Publication number: 20090291471
    Abstract: The present invention provides a novel ?-galactoside-?2,6-sialyltransferase having high productivity and/or high activity, and a nucleic acid encoding the sialyltransferase. The present invention also provides a microorganism producing the sialyltransferase. The present invention further provides a vector carrying a nucleic acid encoding the sialyltransferase, and a host cell transformed with the vector, as well as a method for producing a recombinant ?-galactoside-?2,6-sialyltransferase.
    Type: Application
    Filed: August 10, 2006
    Publication date: November 26, 2009
    Applicant: Japan Tobacco Inc.
    Inventors: Hiroshi Tsukamoto, Toshiki Mine, Yoshimitsu Takakura, Takeshi Yamamoto
  • Cold-inducible promoter sequences
    Patent number: 6084089
    Abstract: This invention discloses a novel cold-inducible promoter which induces gene expression at low temperatures in potato tubers but which is scarcely induced in organs other than tuber or at normal temperature, which induces gene expression for a long time not less than five months.
    Type: Grant
    Filed: October 27, 1997
    Date of Patent: July 4, 2000
    Assignee: Japan Tobacco, Inc.
    Inventors: Toshiki Mine, Akio Ohyama, Toru Hiyoshi, Keisuke Kasaoka
  • DNA encoding ATP-dependent fructose 6-phosphate 1-phosphotransferase originating from plant, recombinant vector containing the same and method for changing sugar content in plant cells under low temperature
    Patent number: 5824862
    Abstract: A DNA encoding cold stable PFK, a recombinant vector which can express a cold stable PFK in a host cell, and a method for changing sugar content in plant cells under low temperature using the recombinant vector are disclosed. The present invention provides a DNA encoding ATP dependent fructose 6-phosphate 1-phosphotransferase originating from a plant, as well as a recombinant vector comprising the DNA and a plant transformed with the DNA.
    Type: Grant
    Filed: June 9, 1995
    Date of Patent: October 20, 1998
    Assignee: Japan Tobacco Inc.
    Inventors: Toru Hiyoshi, Toshiki Mine, Keisuke Kasaoka, Robert Huw Tyson, Anthony Miles John Page