Abstract: A simulated moving-bed type chromatographic separation method separating a weakly adsorptive component, a strongly adsorptive component, and an intermediately adsorptive component, with eluents by using a circulation system in which a plurality of unit packed columns packed with an adsorbent are connected in series and in an endless form via pipes in which a feed solution supply port F, two or more eluent supply ports D corresponding to the eluents, an extraction port A of a fraction containing the weakly adsorptive component, an extraction port B of a fraction containing the intermediately adsorptive component, and an extraction port C of a fraction containing the strongly adsorptive component are provided in the pipes of the circulation system, and positions of the ports F, A, B, and C are set to have a specified relationship.

Abstract: A simulated moving-bed type chromatographic separation method including separating a weakly adsorptive component, a strongly adsorptive component, and an intermediately adsorptive component that has adsorptive property intermediate between the two components, with respect to an adsorbent, with two or more kinds of eluents by using a circulation system in which a plurality of unit packed columns each packed with an adsorbent are connected in series and in an endless form via pipes, the weakly adsorptive component, the strongly adsorptive component, and the intermediately adsorptive component being contained in a feed solution, wherein a feed solution supply port, eluent suply ports and a plurality of extraction ports are provided on the pipes of the circulation system, and positions of the feed solution supply port and the plurality of extraction ports are set to have a specified relationship; and a chromatographic separation system suitable for implementing the chromatographic separation method.

Abstract: The purpose of the present invention is to provide a method for identifying antibody CDR3 clusters using high speed in vitro screening a library selected from the group consisting of cDNA library and nucleic acid aptamer library comprising: (i) preparing a positive spherical shaped structure by binding a target molecule to a spherical shaped molecule, wherein the target molecule is immobilized on the positive spherical shaped structure, wherein the positive spherical shaped structure may contain a fluorescent label; (ii) preparing a negative spherical shaped structure, wherein the target molecule is not immobilized on the negative spherical shaped structure, wherein the negative spherical shaped structure may contain a fluorescent label; (iii) forming a positive spherical shaped conjugate or a negative spherical shaped conjugate by binding a target detecting molecule capable of binding to the target molecule to the positive spherical shaped structure or to the negative spherical structure, wherein the target

Abstract: A simulated moving-bed type chromatographic separation method separating a weakly adsorptive component, a strongly adsorptive component, and an intermediately adsorptive component, with eluents by using a circulation system in which a plurality of unit packed columns packed with an adsorbent are connected in series and in an endless form via pipes in which a feed solution supply port F, two or more eluent supply ports D corresponding to the eluents, an extraction port A of a fraction containing the weakly adsorptive component, an extraction port B of a fraction containing the intermediately adsorptive component, and an extraction port C of a fraction containing the strongly adsorptive component are provided in the pipes of the circulation system, and positions of the ports F, A, B, and C are set to have a specified relationship.

Abstract: The purpose of the present invention is to provide a method for identifying antibody CDR3 clusters using high speed in vitro screening a library selected from the group consisting of cDNA library and nucleic acid aptamer library comprising: (i) preparing a positive spherical shaped structure by binding a target molecule to a spherical shaped molecule, wherein the target molecule is immobilized on the positive spherical shaped structure, wherein the positive spherical shaped structure may contain a fluorescent label; (ii) preparing a negative spherical shaped structure, wherein the target molecule is not immobilized on the negative spherical shaped structure, wherein the negative spherical shaped structure may contain a fluorescent label; (iii) forming a positive spherical shaped conjugate or a negative spherical shaped conjugate by binding a target detecting molecule capable of binding to the target molecule to the positive spherical shaped structure or to the negative spherical structure, wherein the target

Abstract: The purpose of the present invention is to provide a high-speed in vitro screening method for any library selected from the group consisting of a cDNA display library and a nucleic acid aptamer library. This high-speed in vitro screening method involves: (i) a step for preparing positive spherical structures formed by immobilizing a target molecule on a spherical structure and negative spherical structures having no target molecules immobilized thereon; (ii) a step in which a target detection molecule, selected from the aforementioned library having a library size of greater than or equal to 1010, is bonded on each spherical structure to obtain spherical conjugates; (iii) a step in which the spherical conjugates are sorted into positive spherical conjugates and negative spherical conjugates with a cell sorter; (v) a step for supplying the nucleic acid on the surface of the sorted spherical conjugates for PCR; (vi) and a repetition step for repeating steps (i) to (v) above using DNA obtained by PCR.

Abstract: A continuously variable transmission 1 includes: a primary pulley 20 including a nipping groove 22 receiving a V belt 12 wound across pulleys, the primary pulley 20 including a fixed half pulley 21 having a back side, opposite to the nipping groove 22, provided with a hollow part S; and a reinforcing member 41 having a center hole receiving a driving shaft 11 and tapering from an outer circumference side toward an inner circumference side to have an annular conical shape. The reinforcing member 41 has an outer circumference side end portion 41a brought into contact with and attached to an outer circumference side end surface Sa of the hollow part S and has an inner circumference side end portion 41b attached to an inner circumference side end surface Sb of the hollow part S via a holding member 42 having a wedge-shaped cross section.

Abstract: A water electrolysis system includes a water electrolysis unit; a housing; a ventilation device configured to cause air to flow inside a space of the housing; and a heating device provided on an upstream side of a water electrolysis device in a flow path of the air. A control device controls operation of the ventilation device based on ventilation temperature information detected by a ventilation temperature sensor provided above the water electrolysis device. Furthermore, the control device controls operation of the heating device based on heating temperature information of a heating temperature sensor provided between the heating device and the water electrolysis device in the flow path.

Abstract: The purpose of the present invention is to provide a high-speed in vitro screening method for any library selected from the group consisting of a cDNA display library and a nucleic acid aptamer library. This high-speed in vitro screening method involves: (i) a step for preparing positive spherical structures formed by immobilizing a target molecule on a spherical structure and negative spherical structures having no target molecules immobilized thereon; (ii) a step in which a target detection molecule, selected from the aforementioned library having a library size of greater than or equal to 1010, is bonded on each spherical structure to obtain spherical conjugates; (iii) a step in which the spherical conjugates are sorted into positive spherical conjugates and negative spherical conjugates with a cell sorter; (v) a step for supplying the nucleic acid on the surface of the sorted spherical conjugates for PCR; (vi) and a repetition step for repeating steps (ii) to (v) above using DNA obtained by PCR.

Abstract: A continuously variable transmission 1 includes: a primary pulley 20 including a nipping groove 22 receiving a V belt 12 wound across pulleys, the primary pulley 20 including a fixed half pulley 21 having a back side, opposite to the nipping groove 22, provided with a hollow part S; and a reinforcing member 41 having a center hole receiving a driving shaft 11 and tapering from an outer circumference side toward an inner circumference side to have an annular conical shape. The reinforcing member 41 has an outer circumference side end portion 41a brought into contact with and attached to an outer circumference side end surface Sa of the hollow part S and has an inner circumference side end portion 41b attached to an inner circumference side end surface Sb of the hollow part S via a holding member 42 having a wedge-shaped cross section.

Abstract: A centrifugal pendulum vibration control device for an engine includes an input member, a first link member, a first inertia mass body, a second link member, and a second inertia mass body. The input member is rotated by an engine. The first link member is rotatably connected to the input member. The first inertia mass body is supported on the input member via the first link member so as to perform pendulum motion. The second link member is rotatably connected to the first inertia mass body. The second inertia mass body is supported on the first inertia mass body via the second link member so as to perform pendulum motion. The input member and the second inertia mass body are coaxially disposed so as to be relatively rotatable.

Abstract: A centrifugal pendulum vibration control device for an engine includes an input member, a first link member, a first inertia mass body, a second link member, and a second inertia mass body. The input member is rotated by an engine. The first link member is rotatably connected to the input member. The first inertia mass body is supported on the input member via the first link member so as to perform pendulum motion. The second link member is rotatably connected to the first inertia mass body. The second inertia mass body is supported on the first inertia mass body via the second link member so as to perform pendulum motion. The input member and the second inertia mass body are coaxially disposed so as to be relatively rotatable.