Patents by Inventor Toshio Konomi

Toshio Konomi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Acetylesterases
    Patent number: 4517299
    Abstract: New acetylesterases are prepared from Aureobasidium pullulans IFO 4466 which are capable of hydrolyzing cephalosporin C to deacetylcephalosporin C.
    Type: Grant
    Filed: June 3, 1983
    Date of Patent: May 14, 1985
    Assignee: Fujisawa Pharmaceutical Co., Ltd.
    Inventors: Hiroshi Imanaka, Toshio Miyoshi, Toshio Konomi, Yoshiaki Kubochi, Seiziro Hattori, Takeshi Kawakita
  • Process for the preparation of deacetylcephalosporin C
    Patent number: 4414328
    Abstract: Deacetylcephalosporin C is converted to cephalosporin C by contact with an acetylesterase produced by Aureobasidium pullanans strain 1F0 4466.
    Type: Grant
    Filed: July 16, 1981
    Date of Patent: November 8, 1983
    Assignee: Fujisawa Pharmaceutical Co., Ltd.
    Inventors: Hiroshi Imanaka, Toshio Miyoshi, Toshio Konomi, Yoshiaki Kubochi, Seiziro Hattori, Takeshi Kawakita
  • Cell-free synthesis of deacetoxycephalosporin C
    Patent number: 4307192
    Abstract: A cell-free process for converting isopenicillin N, .delta.-(L-.alpha.-aminoadipyl)-L-cysteinyl-D-valine (hereinafter "LLD") and 6-substituted derivatives thereof to deacetoxycephalosporin C (DACPC) by the following reaction sequence: ##STR1## is disclosed. In addition to the starting material, the reaction system includes ATP and a fresh extract of Cephalosporium acremonium prepared and used in a manner to preserve the racemase agent or agents necessary for conversion of the isopenicillin N to penicillin N, a necessary intermediate step in the process.
    Type: Grant
    Filed: January 7, 1981
    Date of Patent: December 22, 1981
    Assignee: Massachusetts Institute of Technology
    Inventors: Arnold L. Demain, Toshio Konomi, Jack E. Baldwin
  • Synthesis of isopenicillin derivatives in the absence of living cells
    Patent number: 4248966
    Abstract: Isopenicillin derivatives having antimicrobial properties and a general formula of: ##STR1## where R, R.sub.1, and R.sub.2 are hydrogen, methyl, ethyl, propyl, isopropyl, or halogenated methyl, ethyl, propyl or isopropyl radicals and R.sub.3 is ##STR2## are produced in a cell-free system using an extract from Cephalosporium acremonium. The starting materials for the synthesis consist of tripeptides composed of unsubstituted or .beta.-substituted D-valine, unsubstituted or substituted L cysteine, and L- .alpha.-aminoadipic acid or its analogues. Certain enzymes in the cell-free extract are inactivated so that conversion does not proceed past the isopenicillin stage.
    Type: Grant
    Filed: May 17, 1979
    Date of Patent: February 3, 1981
    Assignee: Massachusetts Institute of Technology
    Inventors: Arnold L. Demain, Toshio Konomi, Jack E. Baldwin