Patents by Inventor Toshio Oda

Toshio Oda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11959109
    Abstract: A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
    Type: Grant
    Filed: July 3, 2023
    Date of Patent: April 16, 2024
    Assignee: SEIKAGAKU CORPORATION
    Inventors: Hikaru Mizumura, Toshio Oda, Shun-ichiro Kawabata
  • Publication number: 20230357742
    Abstract: A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
    Type: Application
    Filed: July 3, 2023
    Publication date: November 9, 2023
    Applicant: Seikagaku Corporation
    Inventors: Hikaru MIZUMURA, Toshio Oda, Shun-Ichiro Kawabata
  • Publication number: 20230193349
    Abstract: Provided is a technology related to a horseshoe crab factor B variant, and also provided is means for performing endotoxin measurement with high sensitivity. A polypeptide having an amino acid sequence in which the amino acid residue at the 193-position in an amino acid sequence of a polypeptide of horseshoe crab factor B is substituted with a cysteine (Cys) residue, is produced. Endotoxin measurement can be carried out with high sensitivity by configuring a Limulus reagent by combining this polypeptide with horseshoe crab factor C.
    Type: Application
    Filed: October 11, 2022
    Publication date: June 22, 2023
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Yuki KOBAYASHI, Hikaru MIZUMURA, Toshio ODA, Shun-ichiro KAWABATA
  • Patent number: 11499177
    Abstract: Provided is a technology related to a horseshoe crab factor B variant, and also provided is means for performing endotoxin measurement with high sensitivity. A polypeptide having an amino acid sequence in which the amino acid residue at the 193-position in an amino acid sequence of a polypeptide of horseshoe crab factor B is substituted with a cysteine (Cys) residue, is produced. Endotoxin measurement can be carried out with high sensitivity by configuring a Limulus reagent by combining this polypeptide with horseshoe crab factor C.
    Type: Grant
    Filed: March 1, 2018
    Date of Patent: November 15, 2022
    Assignee: SEIKAGAKU CORPORATION
    Inventors: Yuki Kobayashi, Hikaru Mizumura, Toshio Oda, Shun-Ichiro Kawabata
  • Publication number: 20220145280
    Abstract: A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
    Type: Application
    Filed: December 17, 2021
    Publication date: May 12, 2022
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Hikaru MIZUMURA, Toshio ODA, Shun-ichiro KAWABATA
  • Patent number: 11236318
    Abstract: A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
    Type: Grant
    Filed: April 30, 2019
    Date of Patent: February 1, 2022
    Assignee: SEIKAGAKU CORPORATION
    Inventors: Hikaru Mizumura, Toshio Oda, Shun-ichiro Kawabata
  • Publication number: 20220018841
    Abstract: A method for rapidly and highly sensitively measuring endotoxin relies on an endotoxin-measuring agent, which includes a factor C derived from Tachypleus tridentatus that does not have His-tag sequence at the C-terminus, a factor B of a horseshoe crab, and a proclotting enzyme of a horseshoe crab. Each of these proteins can be a recombinant protein obtainable by being expressed using a stably expressing cell line of an insect cell as a host.
    Type: Application
    Filed: October 1, 2021
    Publication date: January 20, 2022
    Inventors: Hikaru Mizumura, Maki Aizawa, Toshio Oda
  • Publication number: 20210363564
    Abstract: Provided is a technology related to a horseshoe crab factor B variant, and also provided is means for performing endotoxin measurement with high sensitivity. A polypeptide having an amino acid sequence in which the amino acid residue at the 193-position in an amino acid sequence of a polypeptide of horseshoe crab factor B is substituted with a cysteine (Cys) residue, is produced. Endotoxin measurement can be carried out with high sensitivity by configuring a Limulus reagent by combining this polypeptide with horseshoe crab factor C.
    Type: Application
    Filed: March 1, 2018
    Publication date: November 25, 2021
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Yuki KOBAYASHI, Hikaru MIZUMURA, Toshio ODA, Shun-ichiro KAWABATA
  • Patent number: 11162949
    Abstract: A method for rapidly and highly sensitively measuring endotoxin relies on an endotoxin-measuring agent, which includes a factor C derived from Tachypleus tridentatus that does not have His-tag sequence at the C-terminus, a factor B of a horseshoe crab, and a proclotting enzyme of a horseshoe crab. Each of these proteins can be a recombinant protein obtainable by being expressed using a stably expressing cell line of an insect cell as a host.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: November 2, 2021
    Assignee: Seikagaku Corporation
    Inventors: Hikaru Mizumura, Maki Aizawa, Toshio Oda
  • Publication number: 20210215695
    Abstract: An object of the present invention is to provide a method for enhancing a sensitivity of a current endotoxin measuring reagent employing a recombinant protein to the endotoxin of Helicobacter pylori. The present invention provides a method for enhancing the sensitivity of an endotoxin measuring reagent to the endotoxin of Helicobacter pylori, the endotoxin measuring reagent containing a recombinant protein of horseshoe crab factor C, the method including increasing a content of the recombinant protein of factor C at the time of endotoxin measurement to an amount that is sufficient for enhancing the sensitivity.
    Type: Application
    Filed: September 26, 2019
    Publication date: July 15, 2021
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Norihiko OGURA, Yuki KOBAYASHI, Hikaru MIZUMURA, Toshio ODA
  • Patent number: 10982202
    Abstract: To provide a method for producing a horseshoe crab recombinant Factor C. The horseshoe crab recombinant Factor C is produced through expression thereof by use of mammalian cells such as CHO DG44 and HEK293 as host cells.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: April 20, 2021
    Assignee: SEIKAGAKU CORPORATION
    Inventors: Hikaru Mizumura, Toshio Oda, Shun-ichiro Kawabata
  • Patent number: 10883963
    Abstract: The present invention addresses the problem of providing a means for making highly sensitive and stable measurements possible using electrochemical measurement methods. This problem is resolved by providing: a labeling substance represented by general formula (1) and used to label a substrate; a measurement substrate that is formed by being labeled using the labeling substance; a method of measuring using electrochemical measurement methods that use the measurement substrate; and a reagent kit that includes as components the labeling substance and/or the measurement substrate. (In general formula (1), R1 is either H or an alkyl group (CmH2m+1), m is an integer of 1-4, R2 is an alkyl group (CnH2n+1), and n is an integer of 1-4.
    Type: Grant
    Filed: February 15, 2017
    Date of Patent: January 5, 2021
    Assignees: SEIKAGAKU CORPORATION, TOHOKU UNIVERSITY
    Inventors: Hikaru Mizumura, Toshio Oda, Tomokazu Matsue, Kumi Inoue
  • Publication number: 20200281814
    Abstract: A main object of the present disclosure is to provide a drug storage container enabling simple and reliable preparation of a mixed solution containing a drug and an auxiliary agent, a closing member that is for use in the drug storage container and configured to detachably retain a solid preparation containing a drug or an auxiliary agent, a method for manufacture of the drug storage container, a microbial contaminant test method using the drug storage container, and a solid preparation for preparing a buffer solution.
    Type: Application
    Filed: October 1, 2018
    Publication date: September 10, 2020
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Masanao WATANABE, Makoto ISHII, Toshio ODA, Hikaru MIZUMURA
  • Publication number: 20190270977
    Abstract: A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
    Type: Application
    Filed: April 30, 2019
    Publication date: September 5, 2019
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Hikaru MIZUMURA, Toshio ODA, Shun-ichiro KAWABATA
  • Publication number: 20190256835
    Abstract: A method for measuring endotoxin in a test specimen, the method, comprising mixing an endotoxin assay agent and the test specimen, and measuring progress of a cascade reaction, wherein the endotoxin assay agent comprises a horseshoe crab Factor C recombinant protein having activity of Factor C and a detection substrate, under the conditions specified.
    Type: Application
    Filed: April 30, 2019
    Publication date: August 22, 2019
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Hikaru MIZUMURA, Toshio ODA, Shun-ichiro KAWABATA
  • Publication number: 20190241629
    Abstract: Provided are all full-length recombinant proteins involved in the clotting mechanism of Limulus polyphemus, cDNAs encoding the same, and applications thereof. A recombinant protein containing the amino acid sequence represented by SEQ ID NO: 2 or 4, a recombinant protein containing the amino acid sequence represented by SEQ ID NO: 6, 8, 10, or 12, a recombinant protein containing the amino acid sequence represented by SEQ ID NO: 14, 16, 18, 20, or 22, variants thereof, cDNAs encoding the same, and utilization thereof.
    Type: Application
    Filed: October 18, 2017
    Publication date: August 8, 2019
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Hikaru MIZUMURA, Yuki KOBAYASHI, Toshio ODA
  • Publication number: 20190049408
    Abstract: The present invention addresses the problem of providing a means for making highly sensitive and stable measurements possible using electrochemical measurement methods. This problem is resolved by providing: a labeling substance represented by general formula (1) and used to label a substrate; a measurement substrate that is formed by being labeled using the labeling substance; a method of measuring using electrochemical measurement methods that use the measurement substrate; and a reagent kit that includes as components the labeling substance and/or the measurement substrate. (In general formula (1), R1 is either H or an alkyl group (CmH2m+1), m is an integer of 1-4, R2 is an alkyl group (CnH2n+1), and n is an integer of 1-4.
    Type: Application
    Filed: February 15, 2017
    Publication date: February 14, 2019
    Applicants: SEIKAGAKU CORPORATION, TOHOKU UNIVERSITY
    Inventors: Hikaru MIZUMURA, Toshio ODA, Tomokazu MATSUE, Kumi INOUE
  • Publication number: 20190025309
    Abstract: To provide means for reducing reaction interference observed when AT III is subjected to limulus test, whereby the limulus test of antithrombin III can be carried out at high accuracy. Reaction interference observed when AT III is subjected to limulus test can be reduced by subjecting AT III to a protein inactivation treatment in the co-presence of a divalent metal salt.
    Type: Application
    Filed: October 3, 2018
    Publication date: January 24, 2019
    Applicant: SEIKAGAKU CORPORATION
    Inventors: Maki AIZAWA, Toshio ODA, Jun AKETAGAWA
  • Patent number: 10144923
    Abstract: To provide a method for producing a horseshoe crab recombinant Factor C. The horseshoe crab recombinant Factor C is produced through expression thereof by use of mammalian cells such as CHO DG44 and HEK293 as host cells.
    Type: Grant
    Filed: December 10, 2013
    Date of Patent: December 4, 2018
    Assignee: SEIKAGAKU CORPORATION
    Inventors: Hikaru Mizumura, Toshio Oda, Shun-ichiro Kawabata
  • Patent number: 10132812
    Abstract: To provide means for reducing reaction interference observed when AT III is subjected to limulus test, whereby the limulus test of antithrombin III can be carried out at high accuracy. Reaction interference observed when AT III is subjected to limulus test can be reduced by subjecting AT III to a protein inactivation treatment in the co-presence of a divalent metal salt.
    Type: Grant
    Filed: December 27, 2013
    Date of Patent: November 20, 2018
    Assignee: SEIKAGAKU CORPORATION
    Inventors: Maki Aizawa, Toshio Oda, Jun Aketagawa