Abstract: A method for assaying bilirubin in which a dry reagent is used, which is capable of accelerating the reaction of bilirubin oxidase, and an assay instrument to be used in the method for assaying bilirubin, are provided. The method for assaying bilirubin is characterized in that a biological sample and a bilirubin oxidase-containing dry reagent are mixed first, and then the mixture obtained and a surfactant-containing dry reagent are mixed. An assay instrument (1) to be used in bilirubin assay is characterized in that a bilirubin oxidase and a surfactant are arranged in any of a sample supply part (11), passages (12, 13, 14), and a detection part (15) in a manner such that the bilirubin oxidase is positioned closer to the sample supply part (11) than the surfactant is.

Abstract: A method for assaying bilirubin in which a dry reagent is used, which is capable of accelerating the reaction of bilirubin oxidase, and an assay instrument to be used in the method for assaying bilirubin, are provided. The method for assaying bilirubin is characterized in that a biological sample and a bilirubin oxidase-containing dry reagent are mixed first, and then the mixture obtained and a surfactant-containing dry reagent are mixed. An assay instrument (1) to be used in bilirubin assay is characterized in that a bilirubin oxidase and a surfactant are arranged in any of a sample supply part (11), passages (12, 13, 14), and a detection part (15) in a manner such that the bilirubin oxidase is positioned closer to the sample supply part (11) than the surfactant is.

Abstract: The invention relates to a method of producing an analytical tool having recesses (20) formed therein for moving a sample liquid, a base plate (2) made of polymeric material, and a cover laminated to the base plate (2) to cover the recesses (20). This producing method includes the step of applying a hydrophilic treatment to the inner surfaces of the recesses (20). The hydrophilic treatment includes a primary modifying operation for primarily modifying the properties of the inner surfaces of the recesses (20) by contacting a modifying gas with the inner surfaces of the recesses (20), and a secondary modifying operation for secondarily modifying the properties of the inner surfaces of the recesses (20).

Abstract: The invention relates to a method of producing an analytical tool having recesses (20) formed therein for moving a sample liquid, a base plate (2) made of polymeric material, and a cover laminated to the base plate (2) to cover the recesses (20). This producing method includes the step of applying a hydrophilic treatment to the inner surfaces of the recesses (20). The hydrophilic treatment includes a primary modifying operation for primarily modifying the properties of the inner surfaces of the recesses (20) by contacting a modifying gas with the inner surfaces of the recesses (20), and a secondary modifying operation for secondarily modifying the properties of the inner surfaces of the recesses (20).

Abstract: A method for measuring the presence of polynucleotide in a sample, comprising the steps of: (1) preparing a sample, an agglutinative agent and an agglutination promoter capable of binding to polynucleotide; (2) mixing said sample, said agent and said promoter; and (3) measuring the degree of agglutination of the agent.

Abstract: A method for measuring the concentration of polynucleotide in a sample comprising the steps of: (1) preparing a sample, a Raman scattering active agent capable of bonding to a polynucleotide and a surface-enhanced Raman scattering (SERS) active substrate capable of capturing the agent; (2) mixing the sample, the agent and the substrate to capture the agent which has not been bonded to polynucleotide on the surface of the substrate; (3) irradiating the mixture; and (4) measuring the SERS radiation which is generated by the agent captured on the surface of the substrate.

Abstract: A cell storing a sample prepared by adsorbing an immunocomplex which is formed by antigen-antibody reaction on a surface of a noble metal colloid such as a gold colloid as an immunity test material is irradiated with excitation light from a light source by an optical system. Raman scattering light generated from the sample is guided to and detected by a spectral detector through an optical system. A signal processing arithmetic unit corrects the Raman scattering light which is detected by the spectral detector with an output of the detector indicating intensity of the light source for obtaining a Raman spectrum, thereby identifying or determining a measuring object material.

Abstract: In order to make a Raman spectral measuring apparatus effective for measurement of a vital sample and attain miniaturization and weight reduction, a near infrared laser diode is employed as a light source, and a spectroscope for receiving Raman scattered light from the sample is formed by a polychrometer comprising a concave diffraction grating and a multichannel photodiode array. The Raman scattered light incident through an inlet slit is separated into its spectral components by the diffraction grating, and its spectra are simultaneously detected by the photodiode array over a prescribed wavelength region.

Abstract: A cell storing a sample prepared by adsorbing an immunocomplex which is formed by antigen-antibody reaction on a surface of a noble metal colloid such as a gold colloid as an immunity test material is irradiated with excitation light from a light source by an optical system. Raman scattering light generated from the sample is guided to and detected by a spectral detector through an optical system. A signal processing arithmetic unit corrects the Raman scattering light which is detected by the spectral detector with an output of the detector indicating intensity of the light source for obtaining a Raman spectrum, thereby identifying or determining a measuring object material.