Abstract: A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell. The method further includes isolating the glycoprotein from the culture medium and contacting the isolated glycoprotein with a sialic acid transferase to add a sialic acid residue attached to the galactose residue in the N-glycan.

Abstract: A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell.

Abstract: An automatic culture apparatus for culturing cells or a tissue with a biological origin in a culture container which is placed in a box-type culture apparatus having a closed and aseptic inner spade, characterized in that a plural number of divided spaces, a gas incubator provided with a practicable window, a unit for supplying a liquid culture medium and a unit for discharging the same, a unit for monitoring the culture conditions, and a transferring unit for continuously or intermittently transferring the culture container toward these units are provided in the box of the culture apparatus, and at least one of the above-described units is equipped with an instruction controlling unit by which the culture apparatus is controlled under an instruction in the form of an electrical signal depending on a data signal generated by the culture condition-monitoring unit so as to prevent cross-contamination.

Abstract: A medium for human cells growing characterized by containing human serum and growth factors, and a method of growing human cells characterized by inoculating the medium with human cells and then growing the cells therein.

Abstract: A method for the secretory production of a glycoprotein having a human-type sugar chain, comprising a step of introducing a gene of an enzyme capable of performing a transfer reaction of a galactose residue to a non-reducing terminal acetylglucosamine residue, and a gene of heterologous glycoprotein, to obtain a transformed plant cell, a step of culturing the plant cell, and a step of recovering the culture medium of the plant cell.