Patents by Inventor Toyoaki Murohara

Toyoaki Murohara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12139699
    Abstract: A magnetic-field generator (10) is provided to be used in construction of a cell sheet (12), and include s a magnetic circuit assembly (24) configured to generate a magnetic field for the construction of the cell sheet (12) using magnetic force caused by the magnetic field wherein the magnetic force is substantially transverse to a plane defined by the cell sheet (12), and a power control system (44) configured to generate and control electric power to magnetically charge the magnetic circuit assembly (24) using the electric power.
    Type: Grant
    Filed: April 13, 2021
    Date of Patent: November 12, 2024
    Assignees: DHF AMERICA, LLC, NATIONAL UNIVERSITY CORPORATION TOKAI NATIONAL HIGHER EDUCATION AND RESEARCH SYSTEM
    Inventors: Kotaro Hirayama, Hotaka Kayano, Shota Tanoue, Yuki Watanabe, Toshio Kokuryo, Rei Shibata, Akira Ito, Toyoaki Murohara
  • Patent number: 11651859
    Abstract: Disclosed is a method comprising acquiring a value relating to VEGF-A of a subject, wherein the value is a measured value of total VEGF-A in a blood sample, or a value obtained by dividing a measured value of VEGF-A165b in the blood sample by a measured value of total VEGF-A in the blood sample (VEGF-A165b/total VEGF-A), and the value suggests prognosis of myocardial infarction of the subject or severity of coronary artery disease of the subject.
    Type: Grant
    Filed: December 20, 2018
    Date of Patent: May 16, 2023
    Assignees: NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY, SYSMEX CORPORATION
    Inventors: Ryosuke Kikuchi, Kazuhiro Harada, Yohei Shibata, Hideki Ishii, Toyoaki Murohara
  • Publication number: 20230126629
    Abstract: A magnetic-field generator (10) is provided to be used in construction of a cell sheet (12), and include s a magnetic circuit assembly (24) configured to generate a magnetic field for the construction of the cell sheet (12) using magnetic force caused by the magnetic field wherein the magnetic force is substantially transverse to a plane defined by the cell sheet (12), and a power control system (44) configured to generate and control electric power to magnetically charge the magnetic circuit assembly (24) using the electric power.
    Type: Application
    Filed: April 13, 2021
    Publication date: April 27, 2023
    Inventors: Kotaro HIRAYAMA, Hotaka KAYANO, Shota TANOUE, Yuki WATANABE, Toshio KOKURYO, Rei SHIBATA, Akira ITO, Toyoaki MUROHARA
  • Publication number: 20200326351
    Abstract: Provided is a method whereby therapeutic effect in a critical limb ischemia patient after angiogenic therapy can be objectively evaluated with minimal patient burden. The problem addressed by the present invention is solved by a method for evaluating a therapeutic effect in a critical limb ischemia patient who has undergone angiogenic therapy. The evaluation method includes, a measurement step for measuring a VEGF-A165b concentration in a blood of the critical limb ischemia patient and an evaluation step for evaluating the therapeutic effect taking changes over time in the measured VEGF-A165b concentration as an indicator.
    Type: Application
    Filed: May 24, 2017
    Publication date: October 15, 2020
    Inventors: Ryosuke KIKUCHI, Kazuhisa KONDO, Toyoaki MUROHARA
  • Publication number: 20190198170
    Abstract: Disclosed is a method comprising acquiring a value relating to VEGF-A of a subject, wherein the value is a measured value of total VEGF-A in a blood sample, or a value obtained by dividing a measured value of VEGF-A165b in the blood sample by a measured value of total VEGF-A in the blood sample (VEGF-A165b/total VEGF-A), and the value suggests prognosis of myocardial infarction of the subject or severity of coronary artery disease of the subject.
    Type: Application
    Filed: December 20, 2018
    Publication date: June 27, 2019
    Applicants: NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY, SYSMEX CORPORATION
    Inventors: Ryosuke KIKUCHI, Kazuhiro Harada, Yohei Shibata, Hideki Ishii, Toyoaki Murohara
  • Publication number: 20180164327
    Abstract: An object of the present embodiment is to provide a testing method by which it is possible to test for reduced renal function even in an early stage. The problem can be solved by a method for testing renal function, wherein the VEGF-A165b content of urine is measured and the measured content is used as an indicator.
    Type: Application
    Filed: May 24, 2016
    Publication date: June 14, 2018
    Inventors: Ryosuke KIKUCHI, Tadashi MATSUSHITA, Toyoaki MUROHARA
  • Publication number: 20160041176
    Abstract: Antibodies are provided that specifically bind to Acting Binding Protein Girdin (Akt Phosphorylation Enhancer), a new substrate of serine/threonine kinase. Additionally, methods of making hybridomas for producing said antibodies and methods of detecting girdin using said antibodies are provided herein. The antibodies and methods are useful for detecting girdin in tissue samples, such as tissue samples from subjects having or suspected of having a disorder in which any of cell motility, cell movement, and angiogenesis is involved.
    Type: Application
    Filed: October 21, 2015
    Publication date: February 11, 2016
    Inventors: Masahide Takahashi, Atsushi Enomoto, Toyoaki Murohara
  • Patent number: 8883732
    Abstract: A therapeutic method for cardiac diseases such as angina, myocardial infarction, arrhythmia (ventricular tachycardia, ventricular fibrillation and atrial fibrillation) is provided. The method is characterized by intracoronary administration of adiponection to mammals.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: November 11, 2014
    Assignee: National University Corporation Nagoya University
    Inventors: Rei Shibata, Toyoaki Murohara
  • Publication number: 20140301988
    Abstract: The present invention addresses the problem of providing a vascular progenitor cell sheet derived from induced pluripotent stem cells, which has the strength to tolerate practical applications and exhibits a high treatment effect. This vascular progenitor cell sheet derived from induced pluripotent stem cells is prepared by performing: (1) a step for preparing magnetically labeled Flk-1 positive cells derived from induced pluripotent stem cells; (2) a step for preparing a mixture of the Flk-1 positive cells and a gel material including type I collagen, laminin, type IV collagen and entactin as active ingredients, and then disseminating the mixture in a culture vessel; (3) a step for drawing the Flk-1 positive cells in the mixture to the culture surface of the culture vessel by application of a magnetic force to form a multi-layered cell layer; and (4) a step for gelling the gel material.
    Type: Application
    Filed: November 7, 2012
    Publication date: October 9, 2014
    Inventors: Toyoaki Murohara, Hiroyuki Honda, Rei Shibata, Masakazu Ishii, Tetsutaro Kito, Hirohiko Suzuki
  • Publication number: 20130217627
    Abstract: A therapeutic method for cardiac diseases such as angina, myocardial infarction, arrhythmia (ventricular tachycardia, ventricular fibrillation and atrial fibrillation) is provided. The method is characterized by intracoronary administration of adiponection to mammals.
    Type: Application
    Filed: March 11, 2013
    Publication date: August 22, 2013
    Applicant: National University Corporation Nagoya University
    Inventors: Rei SHIBATA, Toyoaki MUROHARA
  • Patent number: 7799349
    Abstract: This invention provides methods of forming new blood vessels in diseased or damaged tissue in a subject, methods of increasing blood flow to diseased or damaged tissue in a subject, and methods of increasing angiogenesis in diseased tissue in a subject, which methods comprise: a) isolating autologous bone marrow-mononuclear cells from the subject; and b) transplanting locally into the diseased or damaged tissue an effective amount of the autologous bone-marrow mononuclear cells, thereby forming new blood vessels in the diseased or damaged tissue. The new blood vessels may be capillaries or collateral vessels in ischemic tissue or any site of angiogenesis. Also provided are methods of treating tissue in disease or injury by local transplantation with an effective amount of the autologous bone marrow-mononuclear cells so as to induce vascularization in such diseased tissue.
    Type: Grant
    Filed: February 28, 2005
    Date of Patent: September 21, 2010
    Assignee: Boston Scientific Scimed, Inc.
    Inventors: Takafumi Ueno, Toyoaki Murohara, Keith Allen Robinson, Nicolas A. F. Chronos, Sam Baldwin, Maria Palasis
  • Publication number: 20100050278
    Abstract: It is intended to identity a protein involved in a molecular mechanism between Akt and cell motility as well as to elucidate its function and find its application. In the present teachings, for achieving the above object, protein or partial peptide thereof containing an amino acid sequence identical or substantially identical to the amino acid sequence represented by SEQ ID: 2 is utilized in the screening of a compound or a salt thereof that activates or inhibits any of cell motility, cell migration and angiogenesis.
    Type: Application
    Filed: November 10, 2006
    Publication date: February 25, 2010
    Applicant: NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
    Inventors: Masahide Takahashi, Atsushi Enomoto, Toyoaki Murohara
  • Publication number: 20050147597
    Abstract: This invention provides methods of forming new blood vessels in diseased or damaged tissue in a subject, methods of increasing blood flow to diseased or damaged tissue in a subject, and methods of increasing angiogenesis in diseased tissue in a subject, which methods comprise: a) isolating autologous bone marrow-mononuclear cells from the subject; and b) transplanting locally into the diseased or damaged tissue an effective amount of the autologous bone-marrow mononuclear cells, thereby forming new blood vessels in the diseased or damaged tissue. The new blood vessels may be capillaries or collateral vessels in ischemic tissue or any site of angiogenesis. Also provided are methods of treating tissue in disease or injury by local transplantation with an effective amount of the autologous bone marrow-mononuclear cells so as to induce vascularization in such diseased tissue.
    Type: Application
    Filed: February 28, 2005
    Publication date: July 7, 2005
    Inventors: Takafumi Ueno, Toyoaki Murohara, Keith Robinson, Nicolas Chronos, Sam Baldwin, Maria Palasis
  • Patent number: 6878371
    Abstract: This invention provides methods of forming new blood vessels in diseased or damaged tissue in a subject, methods of increasing blood flow to diseased or damaged tissue in a subject, and methods of increasing angiogenesis in diseased tissue in a subject, which methods comprise: a) isolating autologous bone marrow-mononuclear cells from the subject; and b) transplanting locally into the diseased or damaged tissue an effective amount of the autologous bone-marrow mononuclear cells, thereby forming new blood vessels in the diseased or damaged tissue. The new blood vessels may be capillaries or collateral vessels in ischemic tissue or any site of angiogenesis. Also provided are methods of treating tissue in disease or injury by local transplantation with an effective amount of the autologous bone marrow-mononuclear cells so as to induce vascularization in such diseased tissue.
    Type: Grant
    Filed: July 26, 2001
    Date of Patent: April 12, 2005
    Assignee: Boston Scientific Scimed, Inc.
    Inventors: Takafumi Ueno, Toyoaki Murohara, Keith Allen Robinson, Nicolas A. F. Chronos, Sam Baldwin, Maria Palasis
  • Publication number: 20030180705
    Abstract: A method for regenerating a blood vessel comprising introducing a cell-containing fluid containing vascular endothelial precursor cells and cells to be removed into a cell separator which allows at least the cells to be removed to substantially pass through but substantially captures the vascular endothelial precursor cells; recovering the vascular endothelial precursor cells once captured on said cell separator by introducing a fluid into the said cell separator;
    Type: Application
    Filed: February 27, 2003
    Publication date: September 25, 2003
    Applicant: ASAHI MEDICAL CO., LTD.
    Inventors: Toyoaki Murohara, Mika Aoki, Masaya Sumita, Shuji Terashima, Mikitomo Yasutake
  • Publication number: 20020037278
    Abstract: This invention provides methods of forming new blood vessels in diseased or damaged tissue in a subject, methods of increasing blood flow to diseased or damaged tissue in a subject, and methods of increasing angiogenesis in diseased tissue in a subject, which methods comprise: a) isolating autologous bone marrow-mononuclear cells from the subject; and b) transplanting locally into the diseased or damaged tissue an effective amount of the autologous bone-marrow mononuclear cells, thereby forming new blood vessels in the diseased or damaged tissue. The new blood vessels may be capillaries or collateral vessels in ischemic tissue or any site of angiogenesis. Also provided are methods of treating tissue in disease or injury by local transplantation with an effective amount of the autologous bone marrow-mononuclear cells so as to induce vascularization in such diseased tissue.
    Type: Application
    Filed: July 26, 2001
    Publication date: March 28, 2002
    Inventors: Takafumi Ueno, Toyoaki Murohara, Keith Allen Robinson, Nicolas A.F. Chronos, Sam Baldwin, Maria Palasis