Patents by Inventor Toyokazu Yokomaku

Toyokazu Yokomaku has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
    Patent number: 7429655
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
    Type: Grant
    Filed: October 14, 2003
    Date of Patent: September 30, 2008
    Assignees: Kankyo Engineering Co., Ltd., National Institute of Advanced Industrial Science and Technology
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Methods for monitoring PCR amplification with fluorescently labeled probes or primers for which fluorescence changes upon hybridization to a target
    Patent number: 7419786
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
    Type: Grant
    Filed: January 27, 2006
    Date of Patent: September 2, 2008
    Assignees: Japan Bioindustry Association, Japan as represented by Secretary of Agency of Industrial Science and Technology, KANKYO ENGINEERING Co., Ltd.
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method
    Patent number: 7354707
    Abstract: Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.
    Type: Grant
    Filed: June 27, 2001
    Date of Patent: April 8, 2008
    Assignees: National Institute of Advanced Industrial Science and Technology, Kankyo Engineering Co., Ltd.
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku
  • Nucleic acid probe and novel method of assaying nucleic acid using the same
    Patent number: 7273700
    Abstract: A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.
    Type: Grant
    Filed: March 27, 2002
    Date of Patent: September 25, 2007
    Assignees: National Institute of Advanced Industrial Science and Technology, Kankyo Engineering Co., Ltd.
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku
  • Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
    Patent number: 7094540
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
    Type: Grant
    Filed: August 1, 2002
    Date of Patent: August 22, 2006
    Assignees: Japan Bioindustry Association, Agency of Industrial Science and Technology, Kankyo Engineering Co., Ltd.
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
    Publication number: 20060177856
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
    Type: Application
    Filed: January 27, 2006
    Publication date: August 10, 2006
    Applicants: Japan Bioindustry Association, Japan as represented by Secretary..., KANKYO ENGINEERING Co., Ltd.
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Novel nucleic acid probe and novel method of assaying nucleic acid using the same
    Publication number: 20050048485
    Abstract: A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.
    Type: Application
    Filed: March 27, 2002
    Publication date: March 3, 2005
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku
  • Method for determining a concentration of traget nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
    Publication number: 20040063137
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye.
    Type: Application
    Filed: October 14, 2003
    Publication date: April 1, 2004
    Applicants: KANKYO ENGINEERING Co., Ltd., NAT. INST. OF ADVANCED INDUSTRIAL SCI. AND TECH.
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
    Patent number: 6699661
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
    Type: Grant
    Filed: April 20, 2000
    Date of Patent: March 2, 2004
    Assignees: Kankyo Engineering Co., Ltd., National Institute of Advanced Industrial Science and Technology
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
    Publication number: 20030082592
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye.
    Type: Application
    Filed: August 1, 2002
    Publication date: May 1, 2003
    Applicant: Japan Bioindustry Association
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
    Patent number: 6495326
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
    Type: Grant
    Filed: November 29, 2000
    Date of Patent: December 17, 2002
    Assignees: Japan Bioindustry Association, Secretary of Agency of Industrial Science and Technology, Kankyo Engineering Co., Ltd.
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
    Patent number: 6492121
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye. The method comprises: providing, as the probe, a nucleic acid probe capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target nucleic acid; hybridizing the probe to the target nucleic acid; and measuring a decrease in fluorescence emission from the fluorescent dye after the hybridization relative to fluorescence emission from the fluorescent dye before the hybridization.
    Type: Grant
    Filed: November 29, 2000
    Date of Patent: December 10, 2002
    Assignees: Japan Bioindustry Association, Japan as represented by Secretary of Agency of Industrial Science and Technology, Kankyo Engineering Co., Ltd.
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Novel nucleic acid probes, method for determining concentrations of nucleic acid by using the probes, and method for analyzing data obtained by the method
    Publication number: 20020106653
    Abstract: Nucleic acid probes are provided, each of which is formed of a single-stranded oligonucleotide which can hybridize to a target nucleic acid and is labeled with a fluorescent dye or with a fluorescent dye and a quencher substance. The nucleic acid probes can be easily designed, permit determination, polymorphous analysis or real-time quantitative PCR of nucleic acids in short time, and are not dissociated during reactions. Nucleic acid determination methods, polymorphous analysis methods and real-time quantitative PCR methods, which make use of the nucleic acid probes, are also provided.
    Type: Application
    Filed: June 27, 2001
    Publication date: August 8, 2002
    Applicant: Nat' l Inst. of advan. Industrial Science and Tech
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Masaki Torimura, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku
  • Method for determining a concentration of target nuleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
    Publication number: 20010000148
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye.
    Type: Application
    Filed: November 29, 2000
    Publication date: April 5, 2001
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho
  • Method for determining a concentration of target nuleic acid molecules, nucleic acid probes for the method, and method for analyzing data obtained by the method
    Publication number: 20010000175
    Abstract: A method is provided for determining a concentration of a target nucleic acid by using a nucleic acid probe labeled with a fluorescent dye.
    Type: Application
    Filed: November 29, 2000
    Publication date: April 5, 2001
    Inventors: Ryuichiro Kurane, Takahiro Kanagawa, Yoichi Kamagata, Shinya Kurata, Kazutaka Yamada, Toyokazu Yokomaku, Osamu Koyama, Kenta Furusho