Patents by Inventor Tozo Nishiyama
Tozo Nishiyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240318166Abstract: Plasmid DNA can be efficiently recovered from bacterial cells when producing plasmid DNA using E. coli. Provided is a method for producing plasmid DNA including the following steps (a) to (c): (a) a step of preparing E. coli, which has a mutation in a gene region associated with maintaining outer membrane properties and has a desired plasmid; (b) a step of culturing E. coli of (a); and (c) a step of recovering a desired plasmid from bacterial cells after culture.Type: ApplicationFiled: January 16, 2024Publication date: September 26, 2024Applicants: KANEKA CORPORATION, KANEKA EUROGENTEC S.A., Universite catholique de LouvainInventors: Tomohisa Tokunaga, Tozo Nishiyama, Kazunobu Minakuchi, Seung Hyun Cho, Jean-Francois Collet, Michael Deghelt
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Publication number: 20240052393Abstract: A method to efficiently extract and purify a target foreign protein expressed with the use of an E. coli strain from the periplasm is disclosed. The method for producing a protein comprising the steps (a) to (c) below: (a) a step of introducing a gene encoding a target foreign protein into E. coli having modification in a gene associated with maintenance of the outer membrane structure; (b) a step of culturing the E. coli of (a); and (c) a step of recovering a target foreign protein from the periplasmic fraction of the microbial cell after culture.Type: ApplicationFiled: September 14, 2023Publication date: February 15, 2024Applicant: KANEKA CORPORATIONInventors: Raika Yamagiwa, Tomohisa Tokunaga, Tozo Nishiyama, Kazunobu Minakuchi
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Patent number: 11214809Abstract: A vector is provided that includes a nucleotide sequence selected from any one of (a) to (d). The selection of (a) to (d) includes: (a) the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21, (b) the nucleotide sequence complementary to the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21, where (a) and (b) hybridize under stringent conditions, (c) the nucleotide sequence having 90% or more sequence identity with the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21, and (d) the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21 in which a total of 1 to 50 nucleotides are substituted, deleted, or inserted.Type: GrantFiled: June 1, 2017Date of Patent: January 4, 2022Assignee: KANEKA CORPORATIONInventors: Teruyuki Nishi, Tozo Nishiyama, Toru Watanabe, Yuji Okubo
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Patent number: 10655112Abstract: A polypeptide includes an amino acid sequence selected from the group consisting of: an amino acid sequence having at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 1; and an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by substitution, deletion, and/or addition of one or more amino acid residues. A yeast host expressing the polypeptide in a secretory production system does not add an N-linked sugar chain to the polypeptide, and the polypeptide has endonuclease activity.Type: GrantFiled: September 28, 2018Date of Patent: May 19, 2020Assignee: KANEKA CORPORATIONInventor: Tozo Nishiyama
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Patent number: 10570197Abstract: It is an object of the present invention to provide a method for producing a low-molecular-weight antibody such as a Fab-type antibody, using yeast as a host, wherein the method is able to produce the low-molecular-weight antibody with high productivity. According to the present invention, there is provided a gene comprising a nucleotide sequence encoding an amino acid or an amino acid sequence capable of increasing the secretion amount of a Fab-type antibody at the 3?-terminus of a nucleotide sequence encoding the amino acid sequence of the Fd chain or L chain of an antibody.Type: GrantFiled: October 23, 2015Date of Patent: February 25, 2020Assignee: KANEKA CORPORATIONInventor: Tozo Nishiyama
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Publication number: 20190017036Abstract: A polypeptide includes an amino acid sequence selected from the group consisting of: an amino acid sequence having at least 85% sequence identity to the amino acid sequence of SEQ ID NO: 1; and an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by substitution, deletion, and/or addition of one or more amino acid residues. A yeast host expressing the polypeptide in a secretory production system does not add an N-linked sugar chain to the polypeptide, and the polypeptide has endonuclease activity.Type: ApplicationFiled: September 28, 2018Publication date: January 17, 2019Applicant: KANEKA CORPORATIONInventor: Tozo Nishiyama
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Patent number: 9783796Abstract: The present invention has its object to provide a novel polypeptide having amidase activity to selectively hydrolyze S-enantiomer in racemic nipecotamide, a DNA encoding the polypeptide, a vector containing the DNA, a transformant transformed with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid in which a racemic carboxylic acid amide is hydrolyzed with the polypeptide or the transformant.Type: GrantFiled: March 14, 2008Date of Patent: October 10, 2017Assignee: KANEKA CORPORATIONInventors: Masutoshi Nojiri, Daisuke Moriyama, Tozo Nishiyama, Naoaki Taoka
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Publication number: 20170268013Abstract: A vector includes a nucleotide sequence according to any one of the following (a) to (d): (a) a nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21; (b) a nucleotide sequence of a nucleic acid that hybridizes with a nucleic acid consisting of a nucleotide sequence complementary to the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21, under stringent conditions; (c) a nucleotide sequence having 85% or more sequence identity with the nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21; (d) a nucleotide sequence set forth in SEQ ID NO: 12, 15, 18, or 21 in which one or more nucleotides are substituted, deleted, inserted, and/or added.Type: ApplicationFiled: June 1, 2017Publication date: September 21, 2017Applicant: Kaneka CorporationInventors: Teruyuki Nishi, Tozo Nishiyama, Toru Watanabe, Yuji Okubo
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Patent number: 9464306Abstract: The present invention relates to a method for producing an L-amino acid by reacting an enantiomeric mixture of an N-succinyl amino acid with L-succinylase in the presence of N-acylamino acid racemase to specifically hydrolyze the L-form. In particular, the present invention relates to a method for producing an L-amino acid in high yield by using an N-succinyl amino acid whose dissolved concentration is particularly low as a raw material to perform a reaction while precipitating the produced L-amino acid out of the reaction system. The present invention enables efficient production of an L-amino acid having high optical purity, particularly an L-amino acid useful as a raw material for products such as pharmaceutical products and agricultural chemicals.Type: GrantFiled: October 28, 2009Date of Patent: October 11, 2016Assignee: KANEKA CORPORATIONInventors: Masutoshi Nojiri, Tozo Nishiyama, Naoaki Taoka
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Patent number: 9376667Abstract: Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.Type: GrantFiled: February 1, 2016Date of Patent: June 28, 2016Assignee: Kaneka CorporationInventors: Shinichi Yoshida, Akira Iwasaki, Motohisa Washida, Tozo Nishiyama, Daisuke Moriyama, Naoaki Taoka
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Publication number: 20160145585Abstract: Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.Type: ApplicationFiled: February 1, 2016Publication date: May 26, 2016Inventors: Shinichi Yoshida, Akira Iwasaki, Motohisa Washida, Tozo Nishiyama, Daisuke Moriyama, Naoaki Taoka
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Publication number: 20160122428Abstract: It is an object of the present invention to provide a method for producing a low-molecular-weight antibody such as a Fab-type antibody, using yeast as a host, wherein the method is able to produce the low-molecular-weight antibody with high productivity. According to the present invention, there is provided a gene comprising a nucleotide sequence encoding an amino acid or an amino acid sequence capable of increasing the secretion amount of a Fab-type antibody at the 3?-terminus of a nucleotide sequence encoding the amino acid sequence of the Fd chain or L chain of an antibody.Type: ApplicationFiled: October 23, 2015Publication date: May 5, 2016Applicant: KANEKA CORPORATIONInventor: Tozo NISHIYAMA
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Patent number: 9315782Abstract: Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.Type: GrantFiled: January 19, 2011Date of Patent: April 19, 2016Assignee: Kaneka CorporationInventors: Shinichi Yoshida, Akira Iwasaki, Motohisa Washida, Tozo Nishiyama, Daisuke Moriyama, Naoaki Taoka
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Patent number: 9102969Abstract: An object of the present invention it to improve the secretion productivity of a heterologous protein in a methanol-assimilating yeast. The present invention provides a method for producing a heterologous protein, characterized by comprising using a methanol-assimilating yeast with disruption of the VPS gene as a host for secretory production of the heterologous protein.Type: GrantFiled: March 7, 2012Date of Patent: August 11, 2015Assignee: KANEKA CORPORATIONInventors: Tozo Nishiyama, Yasuyoshi Sakai
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Publication number: 20140106404Abstract: An object of the present invention it to improve the secretion productivity of a heterologous protein in a methanol-assimilating yeast. The present invention provides a method for producing a heterologous protein, characterized by comprising using a methanol-assimilating yeast with disruption of the VPS gene as a host for secretory production of the heterologous protein.Type: ApplicationFiled: March 7, 2012Publication date: April 17, 2014Applicant: KANEKA CORPORATIONInventors: Tozo Nishiyama, Yasuyoshi Sakai
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Publication number: 20130059348Abstract: The present invention has its object to provide a novel polypeptide having amidase activity to selectively hydrolyze S-enantiomer in racemic nipecotamide, a DNA encoding the polypeptide, a vector containing the DNA, a transformant transformed with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid in which a racemic carboxylic acid amide is hydrolyzed with the polypeptide or the transformant.Type: ApplicationFiled: March 14, 2008Publication date: March 7, 2013Applicant: Kaneka CorporationInventors: Masutoshi Nojiri, Daisuke Moriyama, Tozo Nishiyama, Naoaki Taoka
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Publication number: 20130030164Abstract: Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.Type: ApplicationFiled: January 19, 2011Publication date: January 31, 2013Applicant: Kaneka CorporationInventors: Shinichi Yoshida, Akira Iwasaki, Motohisa Washida, Tozo Nishiyama, Daisuke Moriyama, Naoaki Taoka
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Patent number: 8304216Abstract: The present invention has its object to provide a method of producing an erythro- or threo-2-amino-3-hydroxypropionic acid ester, and so forth. The present invention relates to: a method of asymmetrically reducing an N-2-amino-3-oxopropionic acid ester by allowing cells of a microorganism to act thereon; a polypeptide having an activity of asymmetrically reducing a carbonyl compound to give an optically active alcohol, which is isolated from a microorganism belonging to genus Brevundimonas; a DNA coding for the polypeptide; and a transformant producing the polypeptide. The invention also relates to a method of producing an optically active alcohol by reducing a carbonyl compound with the help of the polypeptide or the transformant.Type: GrantFiled: March 29, 2007Date of Patent: November 6, 2012Assignee: Kaneka CorporationInventors: Tozo Nishiyama, Yoshihiko Yasohara
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Patent number: 8129163Abstract: An object of the present invention is to provide a novel alcohol dehydrogenase, a gene for the alcohol dehydrogenase, a vector including the gene, a transformant transformed with the vector, and a method for producing an optically active alcohol by utilizing them. A feature of the present invention directs to a novel polypeptide isolated from Candida maltosa, a DNA coding for the polypeptide, and a transformant producing the polypeptide. Another feature of the present invention directs to a method for producing an optically-active alcohol by reducing a carbonyl compound with the polypeptide or the transformant.Type: GrantFiled: November 27, 2007Date of Patent: March 6, 2012Assignee: Kaneka CorporationInventors: Shigeru Kawano, Takeru Ishige, Keita Iguchi, Tozo Nishiyama, Yoshihiko Yasohara
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Publication number: 20110229940Abstract: The present invention relates to a method for producing an L-amino acid by reacting an enantiomeric mixture of an N-succinyl amino acid with L-succinylase in the presence of N-acylamino acid racemase to specifically hydrolyze the L-form. In particular, the present invention relates to a method for producing an L-amino acid in high yield by using an N-succinyl amino acid whose dissolved concentration is particularly low as a raw material to perform a reaction while precipitating the produced L-amino acid out of the reaction system. The present invention enables efficient production of an L-amino acid having high optical purity, particularly an L-amino acid useful as a raw material for products such as pharmaceutical products and agricultural chemicals.Type: ApplicationFiled: October 28, 2009Publication date: September 22, 2011Applicant: KANEKA CORPORATIONInventors: Masutoshi Nojiri, Tozo Nishiyama, Naoaki Taoka