Abstract: This invention describes a design for a multiwell plate that contains integrated pumps that are used to stir each well of the plate. The device employs microfluidic logic technology to drive each peristaltic pump. This enables the plates to run autonomously, requiring only a static vacuum supply for power. The devices are entirely constructed out of low-cost polymers, with no electronics, and yet contains simple digital logic circuits to control the pumps. A stack of these plates may be run continuously in a standard cell culture incubator, allowing high-throughput culture of organoids.

Abstract: A device for controlling apical flow to a cell culture includes an apical insert that defines at least one inlet channel extending from an inlet port to an apical feed port and at least one outlet channel extending from an apical effluent port to an outlet port. The apical insert includes a projecting portion configured to extend into a cell culture insert to a depth that is less than a depth of the cell culture insert, and a contact surface configured to maintain a spatial relationship between the projecting portion and the cell culture insert.

Abstract: This invention describes a design for a multiwell plate that contains integrated pumps that are used to stir each well of the plate. The device employs microfluidic logic technology to drive each peristaltic pump. This enables the plates to run autonomously, requiring only a static vacuum supply for power. The devices are entirely constructed out of low-cost polymers, with no electronics, and yet contains simple digital logic circuits to control the pumps. A stack of these plates may be run continuously in a standard cell culture incubator, allowing high-throughput culture of organoids.

Abstract: Microfluidic oscillator circuits and pumps for microfluidic devices are provided. The microfluidic pump may include a plurality of fluid valves and a microfluidic oscillator circuit having an oscillation frequency. The fluid valves may be configured to move fluids. Each fluid valve may be connected to a node of the microfluidic oscillator circuit. The pumps may be driven by the oscillator circuits such that fluid movement is accomplished entirely by circuits on a microfluidic chip, without the need for off-chip controls.

Abstract: A device for controlling apical flow to a cell culture includes an apical insert that defines at least one inlet channel extending from an inlet port to an apical feed port and at least one outlet channel extending from an apical effluent port to an outlet port. The apical insert includes a projecting portion configured to extend into a cell culture insert to a depth that is less than a depth of the cell culture insert, and a contact surface configured to maintain a spatial relationship between the projecting portion and the cell culture insert.

Abstract: Microfluidic oscillator circuits and pumps for microfluidic devices are provided. The microfluidic pump may include a plurality of fluid valves and a microfluidic oscillator circuit having an oscillation frequency. The fluid valves may be configured to move fluids. Each fluid valve may be connected to a node of the microfluidic oscillator circuit. The pumps may be driven by the oscillator circuits such that fluid movement is accomplished entirely by circuits on a microfluidic chip, without the need for off-chip controls.

Abstract: Microfluidic oscillator circuits and pumps for microfluidic devices are provided. The microfluidic pump may include a plurality of fluid valves and a microfluidic oscillator circuit having an oscillation frequency. The fluid valves may be configured to move fluids. Each fluid valve may be connected to a node of the microfluidic oscillator circuit. The pumps may be driven by the oscillator circuits such that fluid movement is accomplished entirely by circuits on a microfluidic chip, without the need for off-chip controls.