Abstract: The particle separation and collection assembly uses cyclonic forces to separate and remove large particles from an airstream and concentrate small particles for sensor/detector technology. This assembly utilizes multiple mini cyclones operating in parallel to reduce the size and velocity of air through the cyclone inlets while maintaining the same fluid or flow rate as compared to one large cyclone. The multiple cyclone system can be arranged in a radial geometry or in a bipolar or uni-polar longitudinal design. The particle separator and collection assembly uses a blower or vacuum pump to draw outside gas into the cyclone particle separator assembly through radial inlets. Vacuum transfer channels, extending the entire length of the assembly, pull gas into the top of the cyclone chambers and out through the bottom apex of cyclone chamber and through the top vortex finder.