Abstract: A bicomponent tissue and seedling culture bottle includes an upper cover portion having an opening facing downward, and a lower container portion having a closed base and suitable for fitly but detachably connecting to the downward opening of the upper cover portion, characterized in that, a T-shaped air vent consisting of a vertical air passage centered at a top portion of the upper cover and two horizontal air passages symmetrically located at and extending from two opposite sides of the vertical air passage to be communicable with the outside air, so that air or gas produced in the culture bottle during the culture process can escape from the air vent and germs or viruses can be prevented from entering into the culture bottle when the seedlings are matured and/or are transplanted and the survival percent of the bottle cultured seedlings can be raised.