Abstract: A microfilter, a manufacturing method thereof, and a microfiltration unit for holding the microfilter are provided. The microfilter has: a non-epoxy based microfilm; and a plurality of microholes provided on the surface of the non-epoxy based microfilm and penetrating therethrough via UV laser ablation, wherein the surface of the non-epoxy based microfilm is patterned into predetermined sections for locating isolated targets and quick enumeration.

Abstract: A method for simultaneously genotyping and quantifying Hepatitis B Virus. Also disclosed are (1) a pair of primers containing, respectively, the sequences of SEQ ID NOs: 13 and 14, SEQ ID NOs: 17 and 14, or SEQ ID NOs: 20 and 6, each primer being 8-50 nucleotides in length; (2) a pair of probes, containing, respectively, the sequences of SEQ ID NOs: 18 and 19, SEQ ID NOs: 15 and 16, or SEQ ID NOs: 21 and 22, each probe being 9-50 nucleotides in length; (3) a nucleic acid obtained from amplification of a Hepatitis B Virus nucleic acid template, containing the sequence selected from SEQ ID NOs: 15, 19, or 22, or its complementary sequence, the nucleic acid being 100-1,000 nucleotides in length.

Abstract: A method for simultaneously identifying a single nucleotide polymorphism in a target nucleic acid from a microorganism and quantifying the target nucleic acid.