Patents by Inventor Tsuguki Komori
Tsuguki Komori has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8026078Abstract: The present invention provides a method of measuring a glycated protein in a sample using a redox reaction, by which the glycated protein can be measured accurately with high sensitivity. In order to remove a glycated amino acid present in the sample other than the glycated protein, the glycated amino acid is degraded in advance by causing a fructosyl amino acid oxidase to act thereon, and thereafter, a fructosyl amino acid oxidase is caused to act on the glycated protein in the presence of a tetrazolium compound and sodium azide to cause a redox reaction. The amount of the glycated protein is determined by measuring the redox reaction. As the glycated protein, glycated hemoglobin is preferable.Type: GrantFiled: January 29, 2003Date of Patent: September 27, 2011Assignee: ARKRAY Inc.Inventors: Satoshi Yonehara, Kaori Ishimaru, Tsuguki Komori
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Patent number: 7485436Abstract: A method of stabilizing an oxidation color former in a solution is provided. N-(carboxymethylaminocarbonyl)-4,4?-bis(dimethylamino) diphenylamine sodium salt as an oxidation color former is stabilized by providing it with at least one of a fructosyl amino acid oxidase (FAOD) and a peroxidase (POD) in the solution. The concentration of the FAOD is in the range from 0.01 to 1.0 g/l or 1 to 100 KU/l, and the concentration of the POD is in the range from 0.01 to 1.0 g/l or 1 to 100 KU/l.Type: GrantFiled: October 9, 2002Date of Patent: February 3, 2009Assignee: Arkray, Inc.Inventors: Yuji Yagi, Tsuguki Komori
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Patent number: 7449305Abstract: The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine.Type: GrantFiled: October 9, 2002Date of Patent: November 11, 2008Assignee: ARKRAY, Inc.Inventors: Satoshi Yonehara, Tsuguki Komori
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Patent number: 7432072Abstract: The present invention provides a method of preventing erroneous color development of N-(carboxymethylaminocarbonyl)-4,4?-bis(dimethylamino)diphenylamine sodium salt as a color-developing substrate, thereby improving the accuracy of measurement utilizing a redox reaction performed using the color-developing substrate. A tetrazolium compound, sodium azide, and the color-developing substrate are added to a sample in the presence of a surfactant. A reaction between an oxidizing substance derived from an analyte in the sample and the color-developing substrate, which develops color by oxidation, is caused by an oxidoreductase. By measuring the color developed, the amount of the oxidizing substance is determined. The concentrations of the respective components in the reaction solution are set so that 0.01 to 1 mmol of the tetrazolium compound, 0.003 to 0.5 mmol of the sodium azide, and 0.006 to 0.Type: GrantFiled: May 2, 2003Date of Patent: October 7, 2008Assignee: Arkray, Inc.Inventors: Satoshi Yonehara, Tsuguki Komori
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Publication number: 20050176086Abstract: The present invention provides a method of preventing erroneous color development of N-(carboxymethylaminocarbonyl)-4,4?-bis(dimethylamino)diphenylamine sodium salt as a color-developing substrate, thereby improving the accuracy of measurement utilizing a redox reaction performed using the color-developing substrate. A tetrazolium compound, sodium azide, and the color-developing substrate are added to a sample in the presence of a surfactant. A reaction between an oxidizing substance derived from an analyte in the sample and the color-developing substrate, which develops color by oxidation, is caused by an oxidoreductase. By measuring the color developed, the amount of the oxidizing substance is determined. The concentrations of the respective components in the reaction solution are set so that 0.01 to 1 mmol of the tetrazolium compound, 0.003 to 0.5 mmol of the sodium azide, and 0.006 to 0.Type: ApplicationFiled: May 2, 2003Publication date: August 11, 2005Applicant: ARKRAY, Inc.Inventors: Satoshi Yonehara, Tsuguki Komori
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Publication number: 20050042709Abstract: The present invention provides a method of measuring a glycated protein in a sample using a redox reaction, by which the glycated protein can be measured accurately with high sensitivity. In order to remove a glycated amino acid present in the sample other than the glycated protein, the glycated amino acid is degraded in advance by causing a fructosyl amino acid oxidase to act thereon, and thereafter, a fructosyl amino acid oxidase is caused to act on the glycated protein in the presence of a tetrazolium compound and sodium azide to cause a redox reaction. The amount of the glycated protein is determined by measuring the redox reaction. As the glycated protein, glycated hemoglobin is preferable.Type: ApplicationFiled: January 29, 2003Publication date: February 24, 2005Inventors: Satoshi Yonehara, Kaori Ishimaru, Tsuguki Komori
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Publication number: 20040248226Abstract: The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine.Type: ApplicationFiled: April 6, 2004Publication date: December 9, 2004Inventors: Satoshi Yonehara, Tsuguki Komori
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Publication number: 20040205900Abstract: A method of stabilizing an oxidation color former in a solution is provided. N-(carboxymethylaminocarbonyl)-4,4′-bis(dimethylamino) diphenylamine sodium salt as an oxidation color former is stabilized by providing it with at least one of a fructosyl amino acid oxidase (FAOD) and a peroxidase (POD) in the solution. The concentration of the FAOD is in the range from 0.01 to 1.0 g/l or 1 to 100 KU/l, and the concentration of the POD is in the range from 0.01 to 1.0 g/l or 1 to 100 KU/l.Type: ApplicationFiled: May 28, 2004Publication date: October 21, 2004Inventors: Yuji Yagi, Tsuguki Komori
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Publication number: 20040063213Abstract: A method of determining an analyte in a sample utilizing a redox reaction is provided, by which a highly reliable determined value can be obtained. In this method, a tetrazolium compound is added to a sample in the presence of a surfactant prior to a redox reaction so as to eliminate an effect of hemoglobin and a hemoglobin degradation product as reducing substances in the sample. Thereafter, a reducing or oxidizing substance derived from an analyte is produced and the amount thereof is determined by the redox reaction. Then, the amount of the analyte in the sample is determined based on the amount of the reducing or oxidizing substance thus determined. According to this method, cloudiness due to the coexistence with the hemoglobin can be prevented by the surfactant and thus an increase in the absorbance due to the cloudiness can be inhibited as shown in FIG. 1. As the surfactant, polyoxyethylene ether and the like can be used.Type: ApplicationFiled: March 20, 2003Publication date: April 1, 2004Inventors: Kaoru Hirai, Tsuguki Komori
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Patent number: 6514720Abstract: A highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a tetrazolium compound is added to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance in the sample, then a reducing substance or an oxidizing substance derived from the analyte is formed, the quantity of the formed substance derived from the analyte is measured by the redox reaction, and the quantity of the analyte is determined from the quantity of the formed substance derived from the analyte. As the tetrazolium compound, for example, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt can be used.Type: GrantFiled: September 5, 2001Date of Patent: February 4, 2003Assignee: Kyoto Daiichi Kagaku Co., Ltd.Inventors: Tsuguki Komori, Satoshi Yonehara
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Patent number: 6352835Abstract: A highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a tetrazolium compound is added to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance in the sample, then a reducing substance or an oxidizing substance derived from the analyte is formed, the quantity of the formed substance derived from the analyte is measured by the redox reaction, and the quantity of the analyte is determined from the quantity of the formed substance derived from the analyte. As the tetrazolium compound, for example, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt can be used.Type: GrantFiled: November 15, 1999Date of Patent: March 5, 2002Assignee: Kyoto Daiichi Kagaku Co. Ltd.Inventors: Tsuguki Komori, Satoshi Yonehara
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Publication number: 20020025546Abstract: A highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a tetrazolium compound is added to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance in the sample, then a reducing substance or an oxidizing substance derived from the analyte is formed, the quantity of the formed substance derived from the analyte is measured by the redox reaction, and the quantity of the analyte is determined from the quantity of the formed substance derived from the analyte. As the tetrazolium compound, for example, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt can be used.Type: ApplicationFiled: September 5, 2001Publication date: February 28, 2002Applicant: Kyoto Daiichi Kagaku Co., Ltd.Inventors: Tsuguki Komori, Satoshi Yonehara
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Patent number: 5985591Abstract: A method for measuring the amount of glycosylated protein comprising the steps of decomposing glycosylated proteins with protease, causing a redox reaction between the decomposed products and an Amadori compound oxidoreductase, and determining the redox reaction so as to measure the amount of glycosylated proteins, wherein the decomposition with the protease is carried out in the presence of at least one substrate selected from the group consisting of metalloporphyrin, cytochrome and diaphorase.Type: GrantFiled: November 19, 1998Date of Patent: November 16, 1999Assignee: Kyoto Daiichi Kagaku Co., Ltd.Inventors: Satoshi Yonehara, Tsuguki Komori
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Patent number: RE43795Abstract: The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine.Type: GrantFiled: October 9, 2002Date of Patent: November 6, 2012Assignee: ARKRAY, Inc.Inventors: Satoshi Yonehara, Tsuguki Komori
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Patent number: RE45074Abstract: The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine.Type: GrantFiled: October 16, 2012Date of Patent: August 12, 2014Assignee: ARKRAY, Inc.Inventors: Satoshi Yonehara, Tsuguki Komori
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Patent number: RE45626Abstract: The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine.Type: GrantFiled: July 9, 2014Date of Patent: July 28, 2015Assignee: ARKRAY, Inc.Inventors: Satoshi Yonehara, Tsuguki Komori
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Patent number: RE46073Abstract: The present invention provides a method of pretreating a sample containing a glycated amine as an analyte, thereby enabling highly reliable measurement of a glycated amine. A glycated amino acid in the sample is degraded by causing a fructosyl amino acid oxidase (FAOD) to act thereon, and thereafter, a FAOD further is caused to act on the glycated amine as the analyte in the sample to cause a redox reaction. The amount of the glycated amine is determined by measuring the redox reaction. The substrate specificity of the FAOD caused to act on the glycated amino acid may be either the same as or different from that of the FAOD caused to act on the glycated amine. When using the same FAOD, a FAOD is caused to act on the glycated amino acid to degrade it, and thereafter, the sample is treated with a protease to inactivate the FAOD and also to degrade the glycated amine.Type: GrantFiled: June 26, 2015Date of Patent: July 19, 2016Assignee: ARKRAY, Inc.Inventors: Satoshi Yonehara, Tsuguki Komori