Patents by Inventor Tsunehiro Aki

Tsunehiro Aki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7888123
    Abstract: An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a method for introducing a transgene into a cell of Labyrinthulomycota, which comprises introducing into a cell of Labyrinthulomycota a recombinant vector comprising a transgene and a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, and then inducing homologous recombination in this homologous nucleotide sequence.
    Type: Grant
    Filed: April 28, 2006
    Date of Patent: February 15, 2011
    Assignee: Fujifilm Corporation
    Inventors: Kazuhisa Ono, Tsunehiro Aki, Seiji Kawamoto
  • Patent number: 7863026
    Abstract: An object of the present invention is to provide a microorganism which has a high ability of producing docosahexaenoic acid. The present invention provides a Thraustochytrium strain which has an ability of producing docosahexaenoic acid, and use thereof.
    Type: Grant
    Filed: July 6, 2007
    Date of Patent: January 4, 2011
    Assignee: FUJIFILM Corporation
    Inventors: Hiroyuki Komazawa, Masayoshi Kojima, Tsunehiro Aki, Kazuhisa Ono, Masayuki Kawakami
  • Patent number: 7863024
    Abstract: The present invention provides a process for producing a highly unsaturated fatty acid-containing lipid which is less expensive than existing ones, which comprises culturing a microorganism belonging to the genus Mortierella with the use of, as a medium carbon source, a saccharified starch, which is less expensive than glucose, does not contribute to an increase in osmotic pressure of the culture medium, and can be utilized by the Mortierella microorganism, and collecting a highly unsaturated fatty acid-containing lipid from the culture.
    Type: Grant
    Filed: April 25, 2003
    Date of Patent: January 4, 2011
    Assignee: Suntory Holdings Limited
    Inventors: Kazuhisa Ono, Tsunehiro Aki, Kenichi Higashiyama
  • Patent number: 7759097
    Abstract: An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a vector for the transformation of Labyrinthulomycota with a transgene, which comprises at least (1) a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, (2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream, and (3) a cloning site for transgene insertion having a promoter sequence located upstream and a terminator sequence located downstream.
    Type: Grant
    Filed: April 28, 2006
    Date of Patent: July 20, 2010
    Assignees: Fujifilm Corporation, Hiroshima University
    Inventors: Kazuhisa Ono, Tsunehiro Aki, Seiji Kawamoto
  • Publication number: 20080009045
    Abstract: An object of the present invention is to provide a microorganism which has a high ability of producing docosahexaenoic acid. The present invention provides a Thraustochytrium strain which has an ability of producing docosahexaenoic acid, and use thereof.
    Type: Application
    Filed: July 6, 2007
    Publication date: January 10, 2008
    Inventors: Hiroyuki Komazawa, Masayoshi Kojima, Tsunehiro Aki, Kazuhisa Ono, Masayuki Kawakami
  • Patent number: 7259006
    Abstract: An object of the present invention is to provide a microorganism which has a high ability of producing docosahexaenoic acid. The present invention provides a Thraustochytrium strain which has an ability of producing docosahexaenoic acid, and use thereof.
    Type: Grant
    Filed: December 24, 2003
    Date of Patent: August 21, 2007
    Assignee: Fujifilm Corporation
    Inventors: Hiroyuki Komazawa, Masayoshi Kojima, Tsunehiro Aki, Kazuhisa Ono, Masayuki Kawakami
  • Publication number: 20060286650
    Abstract: An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a vector for the transformation of Labyrinthulomycota with a transgene, which comprises at least (1) a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, (2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream, and (3) a cloning site for transgene insertion having a promoter sequence located upstream and a terminator sequence located downstream.
    Type: Application
    Filed: April 28, 2006
    Publication date: December 21, 2006
    Applicants: FUJI PHOTO FILM CO., LTD., Hiroshima University
    Inventors: Kazuhisa Ono, Tsunehiro Aki, Seiji Kawamoto
  • Publication number: 20060275904
    Abstract: An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a method for introducing a transgene into a cell of Labyrinthulomycota, which comprises introducing into a cell of Labyrinthulomycota a recombinant vector comprising a transgene and a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, and then inducing homologous recombination in this homologous nucleotide sequence.
    Type: Application
    Filed: April 28, 2006
    Publication date: December 7, 2006
    Applicants: FUJI PHOTO FILM CO., LTD., HIROSHIMA UNIVERSITY
    Inventors: Kazuhisa Ono, Tsunehiro Aki, Seiji Kawamoto
  • Publication number: 20060014268
    Abstract: A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g/L of highly unsaturated fatty acids.
    Type: Application
    Filed: September 22, 2005
    Publication date: January 19, 2006
    Inventors: Osamu Suzuki, Kazuhisa Ono, Seiko Shigeta, Tsunehiro Aki, Kengo Akimoto
  • Patent number: 6958229
    Abstract: A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g/L of highly unsaturated fatty acids.
    Type: Grant
    Filed: September 3, 1999
    Date of Patent: October 25, 2005
    Assignee: Suntory Limited
    Inventors: Osamu Suzuki, Kazuhisa Ono, Seiko Shigeta, Tsunehiro Aki, Kengo Akimoto
  • Publication number: 20050191734
    Abstract: The present invention provides a process for producing a highly unsaturated fatty acid-containing lipid which is less expensive than existing ones, which comprises culturing a microorganism belonging to the genus Mortierella with the use of, as a medium carbon source, a saccharified starch, which is less expensive than glucose, does not contribute to an increase in osmotic pressure of the culture medium, and can be utilized by the Mortierella microorganism, and collecting a highly unsaturated fatty acid-containing lipid from the culture.
    Type: Application
    Filed: April 25, 2003
    Publication date: September 1, 2005
    Applicant: Suntory Limited
    Inventors: Kazuhisa Ono, Tsunehiro Aki, Kenichi Higashiyama
  • Publication number: 20040161831
    Abstract: An object of the present invention is to provide a microorganism which has a high ability of producing docosahexaenoic acid. The present invention provides a Thraustochytrium strain which has an ability of producing docosahexaenoic acid, and use thereof.
    Type: Application
    Filed: December 24, 2003
    Publication date: August 19, 2004
    Applicant: FUJI PHOTO FILM CO., LTD.
    Inventors: Hiroyuki Komazawa, Masayoshi Kojima, Tsunehiro Aki, Kazuhisa Ono, Masayuki Kawakami
  • Publication number: 20020146784
    Abstract: A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g/L of highly unsaturated fatty acids.
    Type: Application
    Filed: September 3, 1999
    Publication date: October 10, 2002
    Inventors: OSAMU SUZUKI, KAZUHISA ONO, SEIKO SHIGETA, TSUNEHIRO AKI, KENGO AKIMOTO