Abstract: The invention relates to a stationary phase medium for adsorption chromatography, which is in form of porous microspheres suitable for being packed into a chromatographic column. The porous microspheres are made of cross-linked polymeric material and formed with interconnected macropores to constitute a porous network. The invented porous microspheres have a characteristic size ratio of porous network diameter to microsphere particle size, and the porous network is in fluid communication with the ambient via multiple openings, so that molecules are convectively transported through the porous network. Accordingly, the invention shows low back pressure and high binding capacity to molecules at high mobile phase velocities.

Abstract: The invention relates to a stationary phase medium for adsorption chromatography, which is in form of porous particles suitable for being packed into a chromatographic column. The porous particles are made of cross-linked polymeric material and formed with interconnected macropores to constitute a porous network, through which a mobile phase fluid may flow in a convective manner. The porous particles are substantially free of diffusive pores and, thus, the mass transfer through the porous network is governed by convection alone. The porous particles are fabricated to have irregular granular configurations with rough outer surfaces, so that the convective flow between the porous particles will not be impeded during chromatography process.