Abstract: A fluorescent microarray analyzer includes a light source for emitting a light beam, a light processing unit for focusing the light beam on the biochip and exciting fluorescent targets on the biochip to produce fluorescence, a focusing lens for focusing the fluorescence on a spectrophotometer, a spectrophotometer for detecting signal of the fluorescence, and an output device for selectively outputting/displaying the signal detected by the spectrophotometer. The resulting signal of the output device does not need to be converted into image data for analysis. For acquiring a more accurate result of detection of signal of fluorescence from the fluorescent targets, the photomultiplier tube of the conventional biochip scanner device is replaced with the spectrophotometer of fluorescent microarray analyzer of the present invention and the filter is removed.

Abstract: A CCD-based biochip reader includes a light source for emitting light beams, a collimating lens for converting the light beams into wide parallel rays of light, passing said wide parallel rays of light through a biochip and exciting fluorescence from fluorescent targets on the biochip, a focusing lens for focusing the fluorescence, a filter for filtering out said parallel rays of light, and a charge-coupled device camera for generating images from said fluorescence. For recording intensity of the fluorescence from the fluorescent targets, images of the charge-coupled device camera is converted into digital data through an image converting device. Wide parallel laser beams are produced by the laser and through the collimating lens, and excite all samples on the biochip with high efficiency at the same time. In addition, time for analysis is saved when fluorescent images of a large area on biochips are collected and analyzed through the charge-coupled device camera.

Abstract: A biochip scanner device includes a light source for emitting a light beam; a light processing unit for focusing the light beam onto the biochip to excite fluorescence from a fluorescent target on the biochip; a filter for filtering off the light beam from the light source; a photomultiplier tube (PMT) for detecting and converting the fluorescence into an electrical signal; and an output device for outputting/displaying the electrical signal detected by the PMT. No conversion of the output signal of the output device into image data is needed. A real-time analysis proceeds while samples are being scanned on the biochip. The biochip scanner device of the present invention reads the electrical signal from PMT directly without processing it into image data and setting lens before the PMT is no longer needed. As a result, the structure of the device is simplified and the cost for production is reduced.

Abstract: This invention is an optical calibration method characterized in calibrating the position of a light-permeable article automatically that is applicable to a light-permeable article positioned on an optical calibrating machine base having a platform for calibration, a transmission end, a receiver end, an adjustable laser device, and a power meter or a spectrometer.