Abstract: Disclosed is a method for producing a fusion protein of human serum albumin and human growth hormone. The method comprises (a) a step of culturing a mammalian cell capable of producing the protein in a serum-free medium and causing the protein to be secreted into the culture fluid, (b) a step of collecting a culture supernatant by removing the mammalian cell from the culture fluid, and (c) a step of purifying the protein from the culture supernatant by using column chromatography using a material to which an antibody having an affinity for the protein is bound as a stationary phase, column chromatography using a material having an affinity for phosphate group as a stationary phase, cation exchange column chromatography, and size exclusion column chromatography.

Abstract: Disclosed is a method for production of a fusion protein in which an antibody and a lysosomal enzyme are fused. The method comprises; (a) a step of culturing mammalian cells producing the fusion protein in a serum-free medium to let the mammalian cells secrete the fusion protein in the culture medium, (b) a step of collecting culture supernatant by removing the mammalian cells from the culture medium, and (c) a step of purifying the fusion protein from the culture supernatant by using a column chromatography employing as a solid phase a material to which a substance having affinity for the fusion protein has been bound, a column chromatography employing as a solid phase a material having affinity for the phosphate group, and a size exclusion column chromatography.

Abstract: The present invention provides a method for producing a ring-rolled material of an Fe—Ni based superalloy which inhibits AGG, has a fine-grained structure having an ASTM grain size number of at least 8, and has high circularity. A method for producing a ring-rolled material of an Fe—Ni based superalloy having a composition of an Alloy 718 comprises: heating a ring-shaped material for ring rolling having the composition, in a temperature range of 900° C. to 980° C., and performing finishing ring rolling, as a finishing ring rolling step; heating the ring-rolled material that has been subjected to the finishing ring rolling, in a temperature range of 980 to 1010° C.; and correcting ellipticalness while expanding a diameter of the ring-rolled material by using a ring expander.

Abstract: The present invention provides a method for producing a ring-rolled material of an Fe—Ni based superalloy which inhibits AGG, has a fine-grained structure having an ASTM grain size number of at least 8, and has high circularity. A method for producing a ring-rolled material of an Fe—Ni based superalloy having a composition of an Alloy 718 comprises: heating a ring-shaped material for ring rolling having the composition, in a temperature range of 900° C. to 980° C., and performing finishing ring rolling, as a finishing ring rolling step; heating the ring-rolled material that has been subjected to the finishing ring rolling, in a temperature range of 980 to 1010° C.; and correcting ellipticalness while expanding a diameter of the ring-rolled material by using a ring expander.

Abstract: A method for producing a ring-rolled material of an Fe—Ni based superalloy, which has a high circularity, can inhibit AGG, and can inhibit grain growth. A method for producing a ring-rolled material of an Fe—Ni based superalloy having a composition of an Alloy 718 comprises: a finishing ring rolling step of heating a ring-shaped material for ring rolling having the composition, in a temperature range of 900° C. to 980° C., and performing finishing ring rolling; and a circularity correcting step of correcting an ellipticalness of the ring-rolled material that has been rolled in the finishing ring rolling step, while expanding a diameter of the ring-rolled material by using a ring expander including a pipe-expanding cone and a pipe-expanding die, wherein the ring-rolled material that has been rolled in the finishing ring rolling step is subjected to circularity correction without being reheated or after having been heated to up to 960° C.

Abstract: Disclosed is a highly efficient method for production of highly pure mutant-type human erythropoietin. The method is for production of mutant-type human erythropoietin, in which a transformed mammalian cell is allowed to produce the mutant-type human erythropoietin, and the supernatant of the culture is subjected to hydrophobic column chromatography, multimodal anion exchange column chromatography, anion exchange column chromatography, phosphate group affinity column chromatography, and gel filtration column chromatography, in this order.

Abstract: A cycle is repeated a plurality of times, which includes a forging process for placing a material to be forged in a lower die and pressing the material to be forged in this state and then separating an upper die from the material to be forged; an elevation process for lifting the material to be forged by using an elevation device to separate the material to be forged from the lower die; a rotation process for rotating the material to be forged around its center by using a rotation device; and a lowering process for placing the material to be forged rotated by the elevation device in the lower die.

Abstract: Provided is a burner tile that has good fire resistance, good heat resistance, good erosion resistance, and good thermal shock resistance. A burner tile 1E includes a molded inorganic fiber product and has a burner main hole 3E. The burner tile 1E includes an inner layer 120 including a combination of inorganic fiber rods 121 and 122 such that the inner layer 120 defines an inner circumferential surface of the main hole 3E, and an outer layer 130 formed by surrounding an outer circumferential surface of the inner layer 120 with the molded inorganic fiber product. Part of the burner tile 1E extending along the inner circumferential surface of the main hole 3E has a high bulk density achieved by highly concentrating an inorganic binder in the inorganic fiber in part including the inner circumferential surface.

Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, (g) to dye-affinity column chromatography, and (h) to gel filtration column chromatography, in the order.

Abstract: Disclosed is a method for production of a fusion protein in which an antibody and a lysosomal enzyme are fused. The method comprises; (a) a step of culturing mammalian cells producing the fusion protein in a serum-free medium to let the mammalian cells secrete the fusion protein in the culture medium, (b) a step of collecting culture supernatant by removing the mammalian cells from the culture medium, and (c) a step of purifying the fusion protein from the culture supernatant by using a column chromatography employing as a solid phase a material to which a substance having affinity for the fusion protein has been bound, a column chromatography employing as a solid phase a material having affinity for the phosphate group, and a size exclusion column chromatography.

Abstract: A ring rolling mill includes: a rotary drive main roll and a mandrel roll, which are for reducing the thickness of and rolling a ring-shaped material from the radial direction; a pair of rotary drive axial rolls for reducing the thickness of and rolling the ring-shaped material from the axial direction; a measuring roll for measuring the diameter of the ring-shaped material during rolling; and a speed control unit for controlling the speed of the axial rolls. The speed control unit is configured to repeat measuring the diameter at predetermined time intervals ?t and comparing a measurement value Lt of the diameter at time t and a measurement value Lt+?t of the diameter at time t+?t, and the speed control unit is further configured to maintain the speed of the axial rolls unchanged upon the result of the comparison being Lt+?t<Lt.

Abstract: Disclosed is a highly efficient method for production of highly pure mutant-type human erythropoietin. The method is for production of mutant-type human erythropoietin, in which a transformed mammalian cell is allowed to produce the mutant-type human erythropoietin, and the supernatant of the culture is subjected to hydrophobic column chromatography, multimodal anion exchange column chromatography, anion exchange column chromatography, phosphate group affinity column chromatography, and gel filtration column chromatography, in this order.

Abstract: The present invention provides a high quality material for ring rolling. The material includes radially outer and inner peripheral surfaces. In the material, a center of gravity on a half section is located so as to be closer to the outer peripheral surface in contact with a main roll than a center of the half section in a thickness direction, a shape of the half section includes a height reducing portion having a height from a center line dividing the half section into halves in a height direction, this height is gradually reduced toward the inner peripheral surface in contact with a mandrel roll, and the shape is formed in a substantially linear symmetry such that the center line is a symmetric axis. A height of the inner peripheral surface is from 20% to 50% of a maximum height of the material.

Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, (g) to dye-affinity column chromatography, and (h) to gel filtration column chromatography, in the order.

Abstract: The inorganic fiber molded body of the present invention is characterized in that the molded body has an extremely light weight, and is free from problems such as scattering of fibers and particulate matters from a surface thereof and environmental pollution such as generation of harmful gases. In addition, the present invention provides an inorganic fiber molded body that is excellent in not only thermal shock resistance and mechanical shock resistance but also a high-speed wind erosion resistance, well-balanced in properties and can be used in the applications of various heat-insulating materials. The present invention relates to an inorganic fiber molded body comprising inorganic fibers and inorganic binder particles and having at least one set of a high-fiber density region and a low-fiber density region, in which a ratio of a content of the binder particles in the high-fiber density region to a content of the binder particles in the low-fiber density region as measured by a predetermined method is 0.

Abstract: A manufacturing method provides a high-quality material for ring rolling. The manufacturing method of the material for ring rolling includes a step of heating a disk-shaped material for hot forging to a hot working temperature, a step of arranging the material for hot forging onto a lower die having a convex portion with a truncated conical shape, a step of forming a thin portion by pressing a center portion of the material for hot forging by using an upper die having a convex portion with a truncated conical shape, and a step of manufacturing a material for ring rolling by removing the thin portion wherein a center of gravity on a half section of the material for ring rolling is located so as to be closer to an outer peripheral surface of the half section than a center of the half section in a thickness direction of the half section.

Abstract: A cycle is repeated a plurality of times, which includes a forging process for placing a material to be forged in a lower die and pressing the material to be forged in this state and then separating an upper die from the material to be forged; an elevation process for lifting the material to be forged by using an elevation device to separate the material to be forged from the lower die; a rotation process for rotating the material to be forged around its center by using a rotation device; and a lowering process for placing the material to be forged rotated by the elevation device in the lower die.

Abstract: Disclosed is a method for production of recombinant human alpha-galactosidase A (rh alpha-Gal A) in a large scale, with a high purity. The method comprises the steps of (a) culturing rh alpha-Gal A-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to anion-exchange column chromatography, (d) to hydrophobic column chromatography, (e) to a column chromatography employing as solid phase a material having affinity for phosphate group, (f) to cation-exchange column chromatography, and (g) to gel filtration column chromatography, in the order.

Abstract: Provided is a burner tile that has good fire resistance, good heat resistance, good erosion resistance, and good thermal shock resistance. A burner tile 1E includes a molded inorganic fiber product and has a burner main hole 3E. The burner tile 1E includes an inner layer 120 including a combination of inorganic fiber rods 121 and 122 such that the inner layer 120 defines an inner circumferential surface of the main hole 3E, and an outer layer 130 formed by surrounding an outer circumferential surface of the inner layer 120 with the molded inorganic fiber product. Part of the burner tile 1E extending along the inner circumferential surface of the main hole 3E has a high bulk density achieved by highly concentrating an inorganic binder in the inorganic fiber in part including the inner circumferential surface.

Abstract: A ring rolling mill includes: a rotary drive main roll and a mandrel roll, which are for reducing the thickness of and rolling a ring-shaped material from the radial direction; a pair of rotary drive axial rolls for reducing the thickness of and rolling the ring-shaped material from the axial direction; a measuring roll for measuring the diameter of the ring-shaped material during rolling; and a speed control unit for controlling the speed of the axial rolls. The speed control unit is configured to repeat measuring the diameter at predetermined time intervals ?t and comparing a measurement value Lt of the diameter at time t and a measurement value Lt+?t of the diameter at time t+?t, and the speed control unit is further configured to maintain the speed of the axial rolls unchanged upon the result of the comparison being Lt+?t<Lt.