Abstract: Plants are created with desired traits by conveniently and rapidly inhibiting methylation of target DNA in plants, without using recombination technology. Scaffold RNA produced by transcription of target DNA is cleaved in an RNA-directed DNA methylation mechanism.

Abstract: According to the present invention, methylation of a target DNA in a plant is suppressed quickly and easily without using recombinant technology, and a plant having desired traits is thereby created. In an RNA-directed DNA methylation mechanism, binding between scaffold RNA produced by transcription of the target DNA and an siRNA-AGO4 complex is inhibited.

Abstract: Plants are created with desired traits by conveniently and rapidly inhibiting methylation of target DNA in plants, without using recombination technology. Scaffold RNA produced by transcription of target DNA is cleaved in an RNA-directed DNA methylation mechanism.

Abstract: A vacuum envelope with a built-in electron source capable of preventing peeling of gate electrodes, to thereby enhance reliability in operation over a long period of time and reducing a manufacturing cost thereof. The gate electrodes each are made of niobium oxide or niobium nitride. Nb for the gate electrode is previously oxidized or nitrided to prevent progress of oxidation of the gate electrode due to release of oxygen from lead oxide contained in a seal material during heating for sealing, resulting in expansion of the gate electrode. An insulating layer is formed on a cathode substrate and the gate electrodes are formed on the insulating layer. Then, the seal material is applied onto the insulating layer so as to cover a part of each of the gate electrodes, so that the cathode substrate may be sealedly joined to an anode substrate.